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1. 发明授权

US08415619B2 Methods and systems for mass spectrometry 有权
标题翻译：质谱方法和系统
公开(公告)号：US08415619B2
公开(公告)日：2013-04-09
申请号：US13296793
申请日：2011-11-15
申请人： David R. Goodlett , Scott R. Heron , Jonathan Cooper
发明人： David R. Goodlett , Scott R. Heron , Jonathan Cooper
IPC分类号： H01J49/26 , G01N23/00
CPC分类号： H01J49/0454 , H01J49/0031
摘要： The present invention relates generally to mass spectrometry. The present invention relates more particularly to methods and systems for use in mass spectrometric identification of a variety of analytes, including high molecular weight species such as proteins. One embodiment of the invention is a method for analyzing an analyte. The method includes nebulizing a suspension of the analyte in a solvent with a surface acoustic wave transducer; and performing mass spectrometry on the nebulized suspension. The surface acoustic wave transducer can be used, for example, to transfer non-volatile peptides and proteins (as well as other analyztes, such as oligonucleotides and polymers) to the gas phase at atmospheric pressure. Nebulization using surface acoustic waves can be conducted in a discontinuous or pulsed mode, similar to that used in MALDI, or in a continuous mode, as in ESI.
摘要翻译：本发明一般涉及质谱法。本发明更具体地涉及用于各种分析物（包括高分子量物质如蛋白质）的质谱鉴定中的方法和系统。本发明的一个实施方案是分析分析物的方法。该方法包括将分析物在溶剂中的悬浮液与表面声波换能器雾化; 并对雾化悬浮液进行质谱分析。例如，表面声波换能器可以用于将大气压下的非挥发性肽和蛋白质（以及其它分析物，例如寡核苷酸和聚合物）转移到气相中。使用表面声波的雾化可以以不连续或脉冲模式进行，类似于在MALDI中使用的模式，或者以连续模式进行，如在ESI中。

2. 发明申请

US20120145890A1 Methods And Systems For Mass Spectrometry 有权
标题翻译：质谱法和系统
公开(公告)号：US20120145890A1
公开(公告)日：2012-06-14
申请号：US13296793
申请日：2011-11-15
申请人： David R. Goodlett , Scott R. Heron , Jonathan Cooper
发明人： David R. Goodlett , Scott R. Heron , Jonathan Cooper
IPC分类号： H01J49/26
CPC分类号： H01J49/0454 , H01J49/0031
摘要： The present invention relates generally to mass spectrometry. The present invention relates more particularly to methods and systems for use in mass spectrometric identification of a variety of analytes, including high molecular weight species such as proteins. One embodiment of the invention is a method for analyzing an analyte. The method includes nebulizing a suspension of the analyte in a solvent with a surface acoustic wave transducer; and performing mass spectrometry on the nebulized suspension. The surface acoustic wave transducer can be used, for example, to transfer non-volatile peptides and proteins (as well as other analyztes, such as oligonucleotides and polymers) to the gas phase at atmospheric pressure. Nebulization using surface acoustic waves can be conducted in a discontinuous or pulsed mode, similar to that used in MALDI, or in a continuous mode, as in ESI.
摘要翻译：本发明一般涉及质谱法。本发明更具体地涉及用于各种分析物（包括高分子量物质如蛋白质）的质谱鉴定中的方法和系统。本发明的一个实施方案是分析分析物的方法。该方法包括将分析物在溶剂中的悬浮液与表面声波换能器雾化; 并对雾化悬浮液进行质谱分析。例如，表面声波换能器可以用于将大气压下的非挥发性肽和蛋白质（以及其它分析物，例如寡核苷酸和聚合物）转移到气相中。使用表面声波的雾化可以以不连续或脉冲模式进行，类似于在MALDI中使用的模式，或者以连续模式进行，如在ESI中。

3. 发明授权

US09273339B2 Methods for identifying bacteria 有权
标题翻译：鉴定细菌的方法
公开(公告)号：US09273339B2
公开(公告)日：2016-03-01
申请号：US13342694
申请日：2012-01-03
申请人： David R. Goodlett , Robert K. Ernst , Ying Sonia Ting , Yu Chieh “Philip” Kao
发明人： David R. Goodlett , Robert K. Ernst , Ying Sonia Ting , Yu Chieh “Philip” Kao
IPC分类号： G01N33/48 , C12Q1/04 , G06F19/14 , G06F19/28 , G06G7/58 , H01J49/00
CPC分类号： C12Q1/04 , G06F19/14 , G06F19/28 , H01J49/004
摘要： The present invention provides methods for identifying bacteria by analysis of lipid A and/or lipoteichoic acid structure and/or mass spectrometry ionization patterns.
摘要翻译：本发明提供了通过分析脂质A和/或脂磷壁酸结构和/或质谱电离模式来鉴定细菌的方法。

4. 发明授权

US06629040B1 Isotope distribution encoded tags for protein identification 失效
标题翻译：用于蛋白质鉴定的同位素分布编码标签
公开(公告)号：US06629040B1
公开(公告)日：2003-09-30
申请号：US09528603
申请日：2000-03-20
申请人： David R. Goodlett , James E. Bruce , Beate Rist , Richard D. Smith , Ruedi Aebersold
发明人： David R. Goodlett , James E. Bruce , Beate Rist , Richard D. Smith , Ruedi Aebersold
IPC分类号： G01N3100
CPC分类号： G01N33/6848 , C12Q1/37 , G01N33/534 , G01N33/6815 , G01N33/6818 , G01N33/6842 , G01N2458/15
摘要： The present invention provides methods and reagents for identifying specific masses of compounds in a complex mixture. In particular, the present invention describes an isotope distribution encoded tag (IDEnT) concept wherein the ability to distinguish an analyte encoded with a non-native isotope from those not encoded with the isotope or from those encoded with a different isotope is used for protein identification, enzyme active site identification, peptide sequencing, and the like. Reagents used in the IDEnT strategy consist of a chemical element with a unique isotope distribution not normally found in the analyte class being studied, chemically bonded to, or an integral part of a chemical reagent with a high selectivity of affinity for specific functional group in the analyte.
摘要翻译：本发明提供用于鉴定复合混合物中化合物的特定质量的方法和试剂。特别地，本发明描述了同位素分布编码标签（IDEnT）概念，其中将用非天然同位素编码的分析物与未用同位素编码的分析物或由不同于同位素编码的分析物区分开的能力用于蛋白质鉴定酶活性位点鉴定，肽测序等。在IDEnT策略中使用的试剂由具有独特同位素分布的化学元素组成，该化学元素通常不被发现在被研究的分析物类别中，化学键合到化学试剂或其化学试剂的组成部分，对于特定官能团具有高亲和力选择性分析物。

5. 发明授权

US06829539B2 Methods for quantification and de novo polypeptide sequencing by mass spectrometry 有权
标题翻译：通过质谱法进行定量和从头多肽测序的方法
公开(公告)号：US06829539B2
公开(公告)日：2004-12-07
申请号：US09835072
申请日：2001-04-13
申请人： David R. Goodlett , Andrew Keller
发明人： David R. Goodlett , Andrew Keller
IPC分类号： G01N3348
CPC分类号： G01N33/6848
摘要： The invention provides a method of determining an amino acid sequence of a parent polypeptide. The method consists of: (a) obtaining mass spectra of two or more differentially labeled polypeptide fragments of a parent polypeptide; (b) assigning a mass and a weighting characteristic to two or more paired signals having a difference in mass corresponding to an integer value of said differential label, the weighting characteristic combining properties of each signal within said paired signals; (c) selecting from the mass spectra a paired signal having the assigned mass and a weighting characteristic distinguishable from non-peptide signals, the assigned mass indicating the mass of a polypeptide fragment within the spectra; (d) determining the difference in mass of the polypeptide fragments; (e) assigning the mass differences a satisfying amino acid name, and (f) orienting the assigned amino acid names. Also provided is a method of determining the amino acid sequence of a polypeptide. The method consists of: (a) constructing a graph from mass spectra of two or more differentially labeled polypeptides, the graph comprising a node with mass m, number of labels n, intensity i, and mass differential of labels &dgr;; (b) creating a node corresponding to a paired signal having masses of about m and about m+n&dgr;, and (c) adding a labeled weighted directed edge to the graph between any two nodes corresponding to a mass of an amino acid, the labeled weighted directed edge combining properties of the paired signals.
摘要翻译：本发明提供了确定亲本多肽的氨基酸序列的方法。该方法包括：（a）获得母体多肽的两个或更多个差异标记的多肽片段的质谱; （b）将质量和加权特性分配给具有与所述差分标签的整数值相对应的质量差异的两个或多个配对信号，所述加权特征组合所述成对信号内的每个信号的特性; （c）从质谱中选择具有分配质量的配对信号和与非肽信号可区分的加权特征，指定的质量指示光谱内的多肽片段的质量; （d）确定多肽片段的质量差异; （e）将质量差异指定为令人满意的氨基酸名称，和（f）定向所分配的氨基酸名称。还提供了确定多肽的氨基酸序列的方法。该方法包括：（a）从两个或更多个差异标记的多肽的质谱图构建图，该图包括质量m，标记数n，强度i和标记δ的质量差的节点; （b）创建对应于具有大约m和大约m + ndelta的质量的成对信号的节点，以及（c）在对应于氨基酸质量的任何两个节点之间的图表中添加标记的加权有向边缘，配对信号的加权有向边组合属性。

6. 发明授权

US08909481B2 Method of mass spectrometry for identifying polypeptides 有权
标题翻译：用于鉴定多肽的质谱法
公开(公告)号：US08909481B2
公开(公告)日：2014-12-09
申请号：US11225265
申请日：2005-09-12
申请人： David R. Goodlett
发明人： David R. Goodlett
IPC分类号： G01N33/48 , G01N33/68
CPC分类号： G01N33/6848 , G01N33/6803 , G01N2570/00
摘要： The invention provides methods for identifying polypeptides. The method can include the steps of simultaneously determining the mass of a subset of parent polypeptides from a population of polypeptides and the mass of fragments of the subset of parent polypeptides; comparing the determined masses to an annotated polypeptide index; and identifying one or more polypeptides of the annotated polypeptide index having the determined masses. The method can further include the steps of determining one or more additional characteristics associated with one or more of the parent polypeptides; comparing the determined characteristics to the annotated polypeptide index; and optionally repeating the steps one or more times, wherein a set of characteristics is determined that identifies a parent polypeptide as a single polypeptide in the annotated polypeptide index. The method can additionally include the step of quantitating the amount of the identified polypeptide in a sample containing the polypeptide.
摘要翻译：本发明提供了鉴定多肽的方法。该方法可以包括以下步骤：同时测定来自多肽群体的母体多肽亚群的质量和母体多肽子集的片段的质量; 将确定的质量与注释的多肽指数进行比较; 以及鉴定具有所确定质量的所述注释多肽指数的一个或多个多肽。该方法还可以包括确定与一个或多个亲本多肽相关联的一个或多个附加特征的步骤; 将确定的特征与注释的多肽指数进行比较; 并且任选地重复所述步骤一次或多次，其中确定在所注释的多肽索引中将母体多肽识别为单个多肽的一组特征。该方法可另外包括定量含有多肽的样品中鉴定的多肽的量的步骤。

7. 发明申请

US20120197535A1 Methods for identifying bacteria 有权
标题翻译：鉴定细菌的方法
公开(公告)号：US20120197535A1
公开(公告)日：2012-08-02
申请号：US13342694
申请日：2012-01-03
申请人： David R. Goodlett , Robert K. Ernst , Ying Sonia Ting , Yu Chieh Philip Kao
发明人： David R. Goodlett , Robert K. Ernst , Ying Sonia Ting , Yu Chieh Philip Kao
IPC分类号： G06F19/00 , H01J49/26
CPC分类号： C12Q1/04 , G06F19/14 , G06F19/28 , H01J49/004
摘要： The present invention provides methods for identifying bacteria by analysis of lipid A and/or lipoteichoic acid structure and/or mass spectrometry ionization patterns.
摘要翻译：本发明提供了通过分析脂质A和/或脂磷壁酸结构和/或质谱电离模式来鉴定细菌的方法。

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