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1. 发明授权

US5128450A Nonglycosylated human interleukin-3 analog proteins 失效
标题翻译：非人类人类白细胞介素-3模拟蛋白
公开(公告)号：US5128450A
公开(公告)日：1992-07-07
申请号：US374667
申请日：1989-06-30
申请人： David L. Urdal , Helmut M. Sassenfeld
发明人： David L. Urdal , Helmut M. Sassenfeld
IPC分类号： A61K38/00 , A61P7/00 , A61P7/06 , C07K1/20 , C07K14/435 , C07K14/52 , C07K14/54 , C07K19/00 , C12N15/09 , C12N15/27 , C12N15/81 , C12P21/02 , C12R1/865
CPC分类号： C07K14/5403 , C12N15/81 , A61K38/00 , C07K2319/02 , Y10S530/824 , Y10S930/141
摘要： The present invention provides a pharmaceutical compositions comprising as active ingredients certain truncated purified human IL-3(Pro.sup.8 Asp.sup.15 Asp.sup.70) analog proteins expressed by transformed yeast of the species Saccharomyces cerevisiae, which when administered to a primate do not result in detectable urticaria.
摘要翻译：本发明提供一种药物组合物，其包含作为活性成分的某些截短的纯化人IL-3（Pro8Asp15Asp70）类似蛋白，其通过酿酒酵母属物种的转化酵母表达，其在施用于灵长类动物时不会导致可检测的荨麻疹。

2. 发明授权

US4532207A Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase 失效
标题翻译：利用带电荷的氨基酸聚合物和外肽酶制备多肽的方法
公开(公告)号：US4532207A
公开(公告)日：1985-07-30
申请号：US471917
申请日：1983-03-03
申请人： Stephen J. Brewer , Helmut M. Sassenfeld
发明人： Stephen J. Brewer , Helmut M. Sassenfeld
IPC分类号： C12N15/09 , C07K14/485 , C12P21/00 , C12P21/02 , C12Q1/37 , C12N15/00 , C12P21/04
CPC分类号： C12P21/02 , C07K14/485 , C12Q1/37 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , Y10S435/803 , Y10S930/12
摘要： A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminants by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

3. 发明授权

US07544784B2 Increased recovery of active proteins 有权
公开(公告)号：US07544784B2
公开(公告)日：2009-06-09
申请号：US11603544
申请日：2006-11-22
申请人： Helmut M. Sassenfeld , Richard L. Remmele, Jr. , Rebecca E. McCoy
发明人： Helmut M. Sassenfeld , Richard L. Remmele, Jr. , Rebecca E. McCoy
IPC分类号： C07K1/14 , C07K1/16 , C07K1/30
CPC分类号： C07K1/1133 , C07K1/1136 , C07K14/70575
摘要： The invention provides methods of increasing yields of desired conformation of proteins. In particular embodiments, the invention includes contacting preparations of a recombinant protein with a reduction/oxidation coupling reagent for a time sufficient to increase the relative proportion of a desired configurational isomer.

4. 发明授权

US07157557B2 Increased recovery of active proteins 有权
标题翻译：增加活性蛋白的回收率
公开(公告)号：US07157557B2
公开(公告)日：2007-01-02
申请号：US10080471
申请日：2002-02-22
申请人： Helmut M. Sassenfeld , Richard L. Remmele, Jr. , Rebecca E. McCoy
发明人： Helmut M. Sassenfeld , Richard L. Remmele, Jr. , Rebecca E. McCoy
IPC分类号： C07K14/715
CPC分类号： C07K1/1133 , C07K1/1136 , C07K14/70575
摘要： The invention provides methods of increasing yields of desired conformation of proteins. In particular embodiments, the invention includes contacting preparations of a recombinant protein with a reduction/oxidation coupling reagent for a time sufficient to increase the relative proportion of a desired configurational isomer.
摘要翻译：本发明提供了提高蛋白质所需构象产量的方法。在具体实施方案中，本发明包括使重组蛋白质的制备物与还原/氧化偶联剂接触足够的时间以增加所需构型异构体的相对比例。

5. 发明授权

US07083948B1 Polypeptide purification reagents and methods for their use 有权
标题翻译：多肽纯化试剂及其使用方法
公开(公告)号：US07083948B1
公开(公告)日：2006-08-01
申请号：US10740880
申请日：2003-12-19
申请人： Helmut M. Sassenfeld , Rebecca E. McCoy
发明人： Helmut M. Sassenfeld , Rebecca E. McCoy
IPC分类号： C12P21/06
CPC分类号： C12P21/06
摘要： The present invention provides polypeptide purification reagents that are, themselves, proteins and methods for using these to purify almost any protein of interest. A polypeptide purification reagent comprises a polypeptide domain that can bind to a protein of interest and a polypeptide domain that confers a propensity to precipitate under conditions where the vast majority of proteins will not precipitate. In addition or as an alternative, a polypeptide purification reagent can comprise amino acid sequences that confer an ability to partition into a phase in a liquid—liquid phase system that the majority of proteins do not partition into. Methods for purifying a protein of interest using such polypeptide purification reagents are also provided. The methods include forming a precipitate comprising the polypeptide purification reagent and the protein of interest under conditions in which the vast majority of proteins do not precipitate and in which the biological activities of both proteins are substantially preserved. The methods further include an optional repurification by one or more reprecipitation steps or by affinity chromatography. In addition or as an alternative, the methods include recovering the protein of interest bound to the polypeptide purification reagent in a phase of a liquid—liquid phase system. The methods further include separating the protein of interest from the polypeptide purification reagent.
摘要翻译：本发明提供多肽纯化试剂，其本身是蛋白质和使用它们纯化几乎任何目的蛋白质的方法。多肽纯化试剂包含能够结合目标蛋白质的多肽结构域和赋予绝大多数蛋白质不沉淀的条件下沉淀倾向的多肽结构域。另外或作为替代方案，多肽纯化试剂可以包含赋予在液 - 液相体系中相分配能力的氨基酸序列，大部分蛋白质不分配到其中。还提供了使用这种多肽纯化试剂纯化目标蛋白质的方法。所述方法包括在绝大多数蛋白质不沉淀的条件下形成包含多肽纯化试剂和目的蛋白质的沉淀物，并且其中两种蛋白质的生物活性基本上被保留。所述方法还包括通过一个或多个再沉淀步骤或通过亲和层析的任选的再纯化。另外或作为替代方案，所述方法包括在液相系统的相中回收与多肽纯化试剂结合的感兴趣的蛋白质。所述方法还包括从多肽纯化试剂中分离目的蛋白质。

6. 发明授权

US4880911A Fused polypeptides and methods for their detection 失效
标题翻译：融合多肽及其检测方法
公开(公告)号：US4880911A
公开(公告)日：1989-11-14
申请号：US732106
申请日：1985-05-09
申请人： Stephen J. Brewer , Helmut M. Sassenfeld
发明人： Stephen J. Brewer , Helmut M. Sassenfeld
IPC分类号： C07K14/485 , C12N15/62 , C12P21/02 , C12Q1/34 , C12Q1/37 , G01N33/68
CPC分类号： C12N15/62 , C07K14/485 , C12P21/02 , C12Q1/34 , C12Q1/37 , G01N33/68 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75
摘要： Fused-Polypeptides comprising a polypeptide product and a covalently bonded charged amino acid polymer are disclosed. Methods of detecting the presence of the charged amino acid polymer are used to quantitate the presence of the fused-polypeptides either in crude or purified solutions. The charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide product. Examples using polyarginine and carboxypeptidase B to produce urogastrone and gamma interferon are included.
摘要翻译：公开了包含多肽产物和共价键合的带电荷的氨基酸聚合物的融合 - 多肽。检测电荷氨基酸聚合物的存在的方法用于定量粗制或纯化溶液中融合多肽的存在。带电荷的氨基酸聚合物使用离开多肽产物的外肽酶水解。包括使用聚精氨酸和羧肽酶B来制备尿胃泌素和γ干扰素的实例。

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