专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06344316B1 Nucleic acid analysis techniques 失效
标题翻译：核酸分析技术
公开(公告)号：US06344316B1
公开(公告)日：2002-02-05
申请号：US08882649
申请日：1997-06-25
申请人： David J. Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen T. Cronin , Danny Lee , Huu M. Tran , Hajime Matsuzaki
发明人： David J. Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen T. Cronin , Danny Lee , Huu M. Tran , Hajime Matsuzaki
IPC分类号： C12Q168
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G06F19/20 , G06F19/22 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译：本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。本发明还提供了一种终止标记核酸的方法。在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。

2. 发明授权

US06333155B1 Exploiting genomics in the search for new drugs 有权
标题翻译：利用基因组学寻找新药
公开(公告)号：US06333155B1
公开(公告)日：2001-12-25
申请号：US09215207
申请日：1998-12-18
申请人： David J. Lockhart , Lisa Wodicka , Ming Hsiu Ho
发明人： David J. Lockhart , Lisa Wodicka , Ming Hsiu Ho
IPC分类号： C12Q168
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C12Q2600/158
摘要： The cellular effects of potentially therapeutic compounds are characterized in mammalian cells and yeast. In the latter case the effects can be characterized on a genome-wide scale by monitoring changes in messenger RNA levels in treated cells with high-density oligonucleotide probe arrays.
摘要翻译：潜在治疗性化合物的细胞效应在哺乳动物细胞和酵母中表征。在后一种情况下，通过用高密度寡核苷酸探针阵列监测处理的细胞中的信使RNA水平的变化，可以在全基因组范围内表征效应。

3. 发明授权

US06573044B1 Methods of using chemical libraries to search for new kinase inhibitors 失效
标题翻译：使用化学文库搜索新的激酶抑制剂的方法
公开(公告)号：US06573044B1
公开(公告)日：2003-06-03
申请号：US09221406
申请日：1998-12-22
申请人： Nathanael S. Gray , Peter Schultz , Lisa Wodicka , Laurent Meijer , David J. Lockhart
发明人： Nathanael S. Gray , Peter Schultz , Lisa Wodicka , Laurent Meijer , David J. Lockhart
IPC分类号： C12Q168
CPC分类号： A61K31/52 , C07D473/16
摘要： The generation of selective inhibitors for specific protein kinases would provide new tools for analyzing signal transduction pathways and possibly new therapeutic agents. We have invented an approach to the development of selective protein kinase inhibitors based on the unexpected binding mode of 2,6,9-trisubstituted purines to the ATP binding site of human CDK2. The most potent inhibitor, purvalanol B (IC50=6 nM), binds with a 30-fold greater affinity than the known CDK2 inhibitor, flavopiridol. The cellular effects of this class of compounds were examined and compared to those of flavopiridol by monitoring changes in mRNA expression levels for all genes in treated cells of Saccharomyces cerevisiae using high-density oligonucleotide probe arrays.
摘要翻译：产生特异性蛋白激酶的选择性抑制剂将为分析信号转导途径和可能的新治疗剂提供新的工具。我们已经发明了一种基于26,9-三取代嘌呤到人CDK2的ATP结合位点的意想不到的结合模式来开发选择性蛋白激酶抑制剂的方法。最有效的抑制剂，纯丙醇B（IC50 = 6nM）以比已知的CDK2抑制剂flavopiridol高30倍的亲和力结合。通过使用高密度寡核苷酸探针阵列监测酿酒酵母的处理细胞中所有基因的mRNA表达水平的变化来检查这类化合物的细胞效应，并与黄曲妥替尔的细胞效应进行比较。

4. 发明授权

US06524800B2 Exploiting genomics in the search for new drugs 有权
公开(公告)号：US06524800B2
公开(公告)日：2003-02-25
申请号：US09900845
申请日：2001-07-06
申请人： David J. Lockhart , Lisa Wodicka , Ming Hsiu Ho
发明人： David J. Lockhart , Lisa Wodicka , Ming Hsiu Ho
IPC分类号： C12Q168
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C12Q2600/158
摘要： The cellular effects of potentially therapeutic compounds are characterized in mammalian cells and yeast. In the latter case the effects can be characterized on a genome-wide scale by monitoring changes in messenger RNA levels in treated cells with high-density oligonucleotide probe arrays.

5. 发明授权

US06638719B1 Genotyping biallelic markers 有权
标题翻译：基因分型双标记
公开(公告)号：US06638719B1
公开(公告)日：2003-10-28
申请号：US09603999
申请日：2000-06-26
申请人： Kevin Gunderson , David J. Lockhart
发明人： Kevin Gunderson , David J. Lockhart
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6858 , C12Q2565/501 , C12Q2535/131 , C12Q2535/125 , C12Q2563/107
摘要： A method is described for determining the genotype of one or more individuals at a biallelic marker. The method employs amplification of a region of genomic DNA using color tagged, allele-specific primers and hybridization of the products to an array of allele-specific probes. The genotype is identified from the pattern of hybridization.
摘要翻译：描述了一种用于确定双重标记物中一个或多个个体的基因型的方法。该方法使用彩色标记的等位基因特异性引物扩增基因组DNA区域，并将产物与等位基因特异性探针阵列进行杂交。从杂交模式中鉴定出基因型。

6. 发明授权

US07060443B2 Methods for testing oligonucleotide arrays 失效
公开(公告)号：US07060443B2
公开(公告)日：2006-06-13
申请号：US10457994
申请日：2003-06-10
申请人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
发明人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
IPC分类号： C12Q1/68 , G01N33/553 , G01N33/552 , G01N33/544 , G01N33/53
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10S435/807
摘要： Methods for testing oligonucleotide arrays are disclosed including methods for testing the efficiency of nucleotide coupling; methods for testing amounts of deprotected oligonucleotides; methods for determining amounts of depurinated oligonucleotides; and methods of detecting the presence of cleavable structural features, such as double-stranded nucleic acids.

7. 发明授权

US06927032B2 Expression monitoring by hybridization to high density oligonucleotide arrays 失效
标题翻译：通过与高密度寡核苷酸阵列杂交进行表达监测
公开(公告)号：US06927032B2
公开(公告)日：2005-08-09
申请号：US10353792
申请日：2003-01-28
申请人： David J. Lockhart , Eugene L. Brown , Gordon G. Wong , Mark Chee , Thomas R. Gingeras
发明人： David J. Lockhart , Eugene L. Brown , Gordon G. Wong , Mark Chee , Thomas R. Gingeras
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N15/14 , G01N37/00 , C07H21/02 , C07H21/04 , C12M1/34 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6837 , G01N15/1475 , C12Q2565/507
摘要： This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.
摘要翻译：本发明提供监测多种基因的表达水平的方法。所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸被定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸探针与衍生自RNA转录物的RNA转录物或核酸互补; 并定量阵列中的杂交核酸。

8. 发明授权

US06576425B2 Methods for testing oligonucleotide arrays 有权
公开(公告)号：US06576425B2
公开(公告)日：2003-06-10
申请号：US09781537
申请日：2001-02-08
申请人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
发明人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10S435/807
摘要： Methods for testing oligonucleotide arrays are disclosed including methods for testing the efficiency of nucleotide coupling; methods for testing amounts of deprotected oligonucleotides; methods for determining amounts of depurinated oligonucleotides; and methods of detecting the presence of cleavable structural features, such as double-stranded nucleic acids.

9. 发明授权

US06238862B1 Methods for testing oligonucleotide arrays 失效
标题翻译：测试寡核苷酸阵列的方法
公开(公告)号：US06238862B1
公开(公告)日：2001-05-29
申请号：US08995265
申请日：1997-12-19
申请人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
发明人： Glenn McGall , Anthony D. Barone , Martin Diggelmann , David J. Lockhart , Ann Maria Caviani Pease , Mark Chee
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10S435/807
摘要： Methods for testing oligonucleotide arrays are disclosed including methods for testing the efficiency of nucleotide coupling; methods for testing amounts of deprotected oligonucleotides; methods for determining amounts of depurinated oligonucleotides; and methods of detecting the presence of cleavable structural features, such as double-stranded nucleic acids.
摘要翻译：公开了测试寡核苷酸阵列的方法，包括测试核苷酸偶联效率的方法; 检测去保护的寡核苷酸的量的方法; 用于测定去酰基化寡核苷酸量的方法; 以及检测可切割结构特征如双链核酸的存在的方法。

10. 发明授权

US06548257B2 Methods of identifying nucleic acid probes to quantify the expression of a target nucleic acid 有权
标题翻译：鉴定核酸探针以量化靶核酸的表达的方法
公开(公告)号：US06548257B2
公开(公告)日：2003-04-15
申请号：US09935365
申请日：2001-08-22
申请人： David J. Lockhart , Eugene L. Brown , Gordon G. Wong , Mark Chee , Thomas R. Gingeras
发明人： David J. Lockhart , Eugene L. Brown , Gordon G. Wong , Mark Chee , Thomas R. Gingeras
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12Q1/6837 , G01N15/1475 , C12Q2565/507
摘要： This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.
摘要翻译：本发明提供监测多种基因的表达水平的方法。所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸被定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且寡核苷酸探针与RNA转录物互补衍生自RNA转录物的核酸; 并定量阵列中的杂交核酸。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式