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1. 发明授权

US4293221A Multidimensional slit-scan flow system 失效
标题翻译：多维狭缝扫描流程系统
公开(公告)号：US4293221A
公开(公告)日：1981-10-06
申请号：US30880
申请日：1979-04-17
申请人： David B. Kay , Leon L. Wheeless, Jr. , James L. Cambier
发明人： David B. Kay , Leon L. Wheeless, Jr. , James L. Cambier
IPC分类号： G01N15/14
CPC分类号： G01N15/1475 , G01N15/147 , G01N2015/1447
摘要： Flow systems for photometric analysis of particles, such as biological cells, particularly for obtaining multidmensional slit-scan type contours, as well as other images. In one broad approach, fluorochrome-stained cells in suspension flow through a focused slit of laser excitation light defining a planar excitation region in the X-Y plane. As each fluorochrome-stained cell flows through the excitation region, fluorescence emissions are generated at the intersection of the excitation region and the cell. To generate a slit-scan type contour along the Z axis, fluorescence emissions are monitored as the cell flows through the excitation region and a plurality of substantially planar parallel cross-sections of the cell along the Z axis are excited to fluorescence. To generate slit-scan type contrours along the cellular X and Y axes, various optical system embodiments define various combinations of cellular linear portions within the Z axis cross-section. The fluorescence or other emissions from these cellular linear portions are then combined by integrating to generate the desired contours. In another broad approach described, a three-dimensional volume of a cell in flow is illuminated with exciting radiation which is not a narrow beam. Three optical systems view a central point in the flow system. The optical systems are slit-imaging and have three respective axes orthogonal to each other and symmetrically located about the stream such that the axis of flow forms equal angles with each optical axis. A photomultiplier tube for each optical system detects the imaged cell fluorescence.
摘要翻译：用于光度分析颗粒的流动系统，例如生物细胞，特别是用于获得多维狭缝扫描型轮廓，以及其它图像。在一个广泛的方法中，悬浮液中的荧光染料染色的细胞通过在X-Y平面中限定平面激发区域的激光激发光的聚焦狭缝流动。当每个荧光染料染色的细胞流过激发区域时，在激发区域和细胞的交叉处产生荧光发射。为了沿Z轴产生狭缝扫描型轮廓，当细胞流过激发区域时，荧光发射被监测，并且沿着Z轴的单元的多个基本上平面的平行横截面被激发成荧光。为了沿着蜂窝X和Y轴产生狭缝扫描类型的控制，各种光学系统实施例限定了在Z轴横截面内的多个线性部分的各种组合。然后通过积分来组合来自这些细胞线性部分的荧光或其它发射物以产生期望的轮廓。在所描述的另一种广泛的方法中，流动中的细胞的三维体积被不是窄波束的激发辐射照射。三个光学系统查看流量系统中的中心点。光学系统是狭缝成像的，并且具有彼此正交的三个相应的轴并且围绕流对称地定位，使得流动轴线与每个光轴形成相等的角度。用于每个光学系统的光电倍增管检测成像的细胞荧光。

2. 发明授权

US4350892A X'-, Y'-, Z'- axis multidimensional slit-scan flow system 失效
标题翻译： X'-，Y'-，Z'-轴多维狭缝扫描流系统
公开(公告)号：US4350892A
公开(公告)日：1982-09-21
申请号：US174280
申请日：1980-07-31
申请人： David B. Kay , Leon L. Wheeless, Jr.
发明人： David B. Kay , Leon L. Wheeless, Jr.
IPC分类号： G01N15/14 , G01N21/64
CPC分类号： G01N15/1434 , G01N15/1459 , G01N2015/1413
摘要： A cytofluorometer for simultaneously obtaining multidimensional slit-scan type fluorescence contours of particles, particularly biological cells, in flow. It is recognized that a cell may be effectively partitioned into orthogonal substantially planar cross sections by means of optical imaging through three slit-imaging optical systems, with each of the optical imaging systems viewing the illuminated central region of the cell. Significantly, all optical axes lie substantially in a plane perpendicular to the flow axis. As a result, the depth of focus required is only that necessary to image directly across the flow stream, rather than obliquely as in previous apparatus. This narrower depth of focus can be directly traded fCONTRACT CLAUSESupport for this invention was received through National Cancer Institute Contract No. N01-CB-33862.
摘要翻译：一种用于同时获得流体中颗粒，特别是生物细胞的多维狭缝扫描型荧光轮廓的细胞荧光计。应当认识到，可以通过三个狭缝成像光学系统的光学成像将单元有效地分割成正交的基本上平面的横截面，其中每个光学成像系统观察单元的照射中心区域。重要的是，所有光轴基本上在垂直于流动轴线的平面中。结果，所需的焦点深度仅仅是在流动流中直接成像，而不是像以前的装置那样倾斜地进行成像。这种较窄的焦点深度可以直接交易，以提高系统分辨率。此外，用于顺序获得一维狭缝扫描型荧光轮廓和多维狭缝扫描型荧光轮廓的两级流式细胞荧光计包括其中共享显微镜物镜的光学系统，另外还产生一维通过从狭缝照射区域收集荧光，沿着Z轴或流动轴的狭缝扫描型荧光轮廓。

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