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1. 发明申请

US20120028257A1 Modified luciola cruciata luciferase gene and protein 有权
标题翻译：修饰的luciola cruciata萤光素酶基因和蛋白质
公开(公告)号：US20120028257A1
公开(公告)日：2012-02-02
申请号：US12928337
申请日：2010-12-09
申请人： Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12Q1/66 , C12N15/12 , C12Q1/68
CPC分类号： C12Y113/12007 , C12N9/0069 , C12Q1/008 , C12Q1/66
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。描述了使用这种新酶测量各种生物代谢功能的测定法。

2. 发明申请

US20120034634A1 MODIFIED LUCIOLA CRUCIATA LUCIFERASE PROTEIN 有权
标题翻译：改良的LUCIOLA CRUCIATA LUCIFERASE蛋白
公开(公告)号：US20120034634A1
公开(公告)日：2012-02-09
申请号：US12800830
申请日：2010-05-24
申请人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12N9/02 , C07H21/04 , C12Q1/66
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。

3. 发明授权

US07723502B2 Modified Luciola cruciata luciferase gene and protein 有权
标题翻译：修饰的Luciola cruciata萤光素酶基因和蛋白质
公开(公告)号：US07723502B2
公开(公告)日：2010-05-25
申请号：US12287561
申请日：2008-10-10
申请人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C07H21/02 , C07H21/04 , C12P21/06 , C12P21/04 , C12N15/00
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。

4. 发明授权

US08450054B2 Modified Luciola cruciata luciferase gene and protein 有权
标题翻译：修饰的Luciola cruciata萤光素酶基因和蛋白质
公开(公告)号：US08450054B2
公开(公告)日：2013-05-28
申请号：US12928337
申请日：2010-12-09
申请人： Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12Q1/00 , C12Q1/66
CPC分类号： C12Y113/12007 , C12N9/0069 , C12Q1/008 , C12Q1/66
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。描述了使用这种新酶测量各种生物代谢功能的测定法。

5. 发明申请

US20100093029A1 MODIFIED LUCIOLA CRUCIATA LUCIFERASE GENE AND PROTEIN 有权
标题翻译：改良的LUCIOLA CRUCIATA LUCIFERASE基因和蛋白质
公开(公告)号：US20100093029A1
公开(公告)日：2010-04-15
申请号：US12287561
申请日：2008-10-10
申请人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12P21/02 , C12N15/12 , C12N15/85 , C12N15/82 , C12N15/74 , C07K14/435
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。

6. 发明授权

US08206961B2 Modified Luciola cruciata luciferase protein 有权
标题翻译：修饰的Luciola cruciata萤光素酶蛋白
公开(公告)号：US08206961B2
公开(公告)日：2012-06-26
申请号：US12800830
申请日：2010-05-24
申请人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12N9/02 , C07K1/00 , C12P21/06
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。

7. 发明申请

US20130252268A1 Enzyme substrates for visualizing acidic organelles 审中-公开
标题翻译：用于显示酸性细胞器的酶底物
公开(公告)号：US20130252268A1
公开(公告)日：2013-09-26
申请号：US13986535
申请日：2013-05-14
申请人： John J. Naleway , Daniel J. Coleman
发明人： John J. Naleway , Daniel J. Coleman
IPC分类号： C12Q1/44 , C12Q1/34
CPC分类号： C12Q1/44 , C12Q1/34 , G01N33/5076 , G01N33/531 , G01N33/573 , G01N33/582
摘要： The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.
摘要翻译：本发明涉及基于细胞器活性的酸性细胞器的可视化。本发明的细胞器底物对于细胞器的酶活性是特异性的并且标记这些细胞器，例如溶酶体，使得它们可见并容易观察。本发明的基板包括产生荧光信号的基板。本发明的荧光酸性细胞器酶底物被设计为在低pH值下提供高荧光，并且被衍生化以允许通过完整细胞的外部和细胞器膜的膜渗透，并且可以用于以非常低的浓度染色细胞。它们可用于以非常低的浓度监测细胞中的酶活性，对活细胞或组织无毒性。

8. 发明申请

US20100233744A1 Enzyme substrates for visualizing acidic organelles 有权
标题翻译：用于显示酸性细胞器的酶底物
公开(公告)号：US20100233744A1
公开(公告)日：2010-09-16
申请号：US12381560
申请日：2009-03-11
申请人： Daniel J. Coleman , John J. Naleway
发明人： Daniel J. Coleman , John J. Naleway
IPC分类号： C12Q1/34
CPC分类号： C12Q1/44 , C12Q1/34 , G01N33/5076 , G01N33/531 , G01N33/573 , G01N33/582
摘要： The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.
摘要翻译：本发明涉及基于细胞器活性的酸性细胞器的可视化。本发明的细胞器底物对于细胞器的酶活性是特异性的并且标记这些细胞器，例如溶酶体，使得它们可见并容易观察。本发明的基板包括产生荧光信号的基板。本发明的荧光酸性细胞器酶底物被设计为在低pH值下提供高荧光，并且被衍生化以允许通过完整细胞的外部和细胞器膜的膜渗透，并且可以用于以非常低的浓度染色细胞。它们可用于以非常低的浓度监测细胞中的酶活性，对活细胞或组织无毒性。

9. 发明授权

US08460862B2 Enzyme substrates for visualizing acidic organelles 有权
公开(公告)号：US08460862B2
公开(公告)日：2013-06-11
申请号：US12381560
申请日：2009-03-11
申请人： Daniel J. Coleman , John J. Naleway
发明人： Daniel J. Coleman , John J. Naleway
IPC分类号： C12Q1/00
CPC分类号： C12Q1/44 , C12Q1/34 , G01N33/5076 , G01N33/531 , G01N33/573 , G01N33/582
摘要： The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.

10. 发明授权

US5624909A Derivatives of triterpenoid acids as inhibitors of cell-adhesion molecules ELAM-1 (e-selectin) and LECAM-1 (l-selectin) 失效
标题翻译：三萜酸的衍生物作为细胞粘附分子的抑制剂ELAM-1（e-选择蛋白）和LECAM-1（l-选择素）
公开(公告)号：US5624909A
公开(公告)日：1997-04-29
申请号：US468888
申请日：1995-06-06
申请人： Narasinga Rao , Mark B. Anderson , John J. Naleway , John H. Musser
发明人： Narasinga Rao , Mark B. Anderson , John J. Naleway , John H. Musser
IPC分类号： G01N33/53 , A61K31/704 , A61K49/00 , A61K51/00 , A61K51/04 , A61P29/00 , C07H15/18 , C07H15/203 , C07H15/207 , C07H15/24 , C07H15/256 , C07H17/04 , C07J63/00 , A01N37/00 , A01N39/00 , A61K31/00 , A61K31/56
CPC分类号： C07J63/008 , A61K51/04 , C07H15/18 , C07H15/203 , C07H15/24 , C07H15/256 , C07H17/04 , A61K2121/00
摘要： Triterpenoid acid derivatives have been found to have structures similar to natural ligands to the extent that these derivatives bind to natural selectin receptors including endothelial leukocyte adhesion molecule-1 (ELAM-1) and leukocyte/endothelial cell adhesion molecule-1 (LECAM-1). The molecules can be administered to the patients by themselves or in pharmaceutical formulations in order to alleviate inflammation and/or treat other abnormalities associated with the excessive binding of leukocytes to endothelial receptors.
摘要翻译：已经发现三萜酸衍生物具有与天然配体相似的结构，使得这些衍生物与天然选择素受体结合，包括内皮白细胞粘附分子-1（ELAM-1）和白细胞/内皮细胞粘附分子-1（LECAM-1）。分子可以自己或以药物制剂施用于患者，以减轻炎症和/或治疗与白细胞与内皮受体的过度结合相关的其它异常。

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