专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050272041A1 Purified plant expansin proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US20050272041A1
公开(公告)日：2005-12-08
申请号：US10670009
申请日：2003-09-24
申请人： Daniel Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C12N9/42 , C07H21/04 , C07K14/415 , C07K14/435 , C12N9/00 , C12N9/64 , C12N15/82 , C12P21/06 , C12Q1/68 , D21C1/00 , D21C5/02 , D21H17/22
CPC分类号： C07K14/415 , C12N9/00 , C12N15/8242 , C12N15/8261 , D21C5/027 , D21H17/22 , Y02A40/146 , Y02W30/648
摘要： This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.
摘要翻译：本发明涉及一类称为膨胀素的新型蛋白质，并提出了与之相关的方法。这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质，称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选用于鉴定全长克隆的cDNA文库。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。

2. 发明授权

US06255466B1 Purified plant expansion proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US06255466B1
公开(公告)日：2001-07-03
申请号：US09092160
申请日：1998-06-05
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07H2102
CPC分类号： C12N9/00 , C07K14/415 , C12N9/2437 , C12N15/8241 , C12N15/8242 , C12N15/8261 , D21H17/22 , Y02A40/146
摘要： The sequence is disclosed both as the nucleic acid sequence SEQ ID NO: 1 and as the corresponding amino acid sequence SEQ ID NO: 7. Five other amino acid sequences are also disclosed, two from rice and three from Arabidopsis, SEQ ID NO: 2 through SEQ ID NO:6, respectively.
摘要翻译：该序列同时被公开为核酸序列SEQ ID NO：1，并作为相应的氨基酸序列SEQ ID NO：7。另外还公开了另外5个氨基酸序列，两个来自水稻，三个来自拟南芥，SEQ ID NO：2 通过SEQ ID NO：6。

3. 发明授权

US5959082A Proteins catalyzing the extension of plant cell walls 失效
标题翻译：催化植物细胞壁延伸的蛋白质
公开(公告)号：US5959082A
公开(公告)日：1999-09-28
申请号：US440517
申请日：1995-05-12
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07K14/415 , C12N9/00 , C12N9/42 , C12N15/82 , D21H17/22 , A61K35/78 , A23J1/14 , C07K17/00
CPC分类号： C12N9/00 , C07K14/415 , C12N15/8241 , C12N15/8242 , C12N15/8261 , C12N9/2437 , D21H17/22
摘要： A new class of proteins and methods related thereto are presented. The proteins, which can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose, are referred to as expansins. Two proteins have been isolated by fractionation techniques from washed wall fragments of cucumber hypocotyls, referred to as "cucumber expansin-29" and "cucumber expansin-30" (abbreviated cEx-29 and cEx-30, with respect to their apparent relative masses as determined by SDS-PAGE). Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice. Expansins appear to be broadly distributed throughout the plant kingdom and can be identified in stem and leaf vegetables (i.e., broccoli, cabbage), fruit and seed vegetables (i.e., tomato), fiber crops and cereals (i.e., corn), and forest and ornamental crops (i.e., cotton). An expansin, generally, is a protein which has at least about 60% sequence similarity with the amino acid sequence shown in SEQ. ID. NO:1, and preferably has at least about 70% sequence similarity with SEQ. ID. NO:1.
摘要翻译：提出了一类新的相关蛋白质和方法。可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化的蛋白质被称为膨胀物。通过分离技术从黄瓜下胚轴的洗涤壁片段（称为“黄瓜扩展蛋白-29”和“黄瓜扩展子30”）（缩写为cEx-29和cEx-30），通过分离技术分离了两种蛋白质，其相对于其表观相对质量为通过SDS-PAGE测定）。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选一个cDNA文库，用于鉴定全长克隆。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。扩展体似乎广泛分布在整个植物界，可以在茎叶蔬菜（即西兰花，卷心菜），水果和种子蔬菜（即番茄），纤维作物和谷类（即玉米），以及森林和观赏作物（即棉花）。通常，扩展蛋白是与SEQ ID NO：1所示氨基酸序列具有至少约60％序列相似性的蛋白质。 ID。 NO：1，优选与SEQ ID NO：1至少约70％的序列相似性。 ID。 NO：1。

4. 发明授权

US07226756B2 Purified plant expansin proteins and DNA encoding same 失效
标题翻译：纯化植物扩增蛋白和编码相同的DNA
公开(公告)号：US07226756B2
公开(公告)日：2007-06-05
申请号：US10670009
申请日：2003-09-24
申请人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark J. Guiltinan , Tatyana Shcherban , Jun Shi
发明人： Daniel J. Cosgrove , Simon McQueen-Mason , Mark J. Guiltinan , Tatyana Shcherban , Jun Shi
IPC分类号： C07K14/415 , C12N15/82 , C12N9/42 , C12N9/00
CPC分类号： C07K14/415 , C12N9/00 , C12N15/8242 , C12N15/8261 , D21C5/027 , D21H17/22 , Y02A40/146 , Y02W30/648
摘要： This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.
摘要翻译：本发明涉及一类称为膨胀素的新型蛋白质，并提出了与之相关的方法。这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质，称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。此外，对来自纯化的cEx-29蛋白的三个肽片段进行测序，然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA，然后使用PCR片段筛选用于鉴定全长克隆的cDNA文库。另外一种扩增蛋白已经从燕麦胚芽鞘（oat expansin oEx-29）中分离出来，而在拟南芥中已经鉴定了三种扩增蛋白序列，另外两种在水稻中被鉴定出来。

5. 发明授权

US07002059B1 Modification of plant fibers 失效
标题翻译：植物纤维的修饰
公开(公告)号：US07002059B1
公开(公告)日：2006-02-21
申请号：US09383579
申请日：1999-08-25
申请人： Michael Meyrick Burrell , Amanda Pat Cambridge , Martin Jack Maunders , Simon McQueen-Mason
发明人： Michael Meyrick Burrell , Amanda Pat Cambridge , Martin Jack Maunders , Simon McQueen-Mason
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8242 , Y02A40/146
摘要： The invention relates to the isolation and characterisation of novel expansin gene sequences from heterologous and homologous tree species and re-introducing such novel genes into trees so as to alter expansin levels. Six novel genes have been identified. Eucalyptus has also been transformed using the cucumber EX29 sequence (GenBank, Accession No. U30382-known as Cs-EXP1) (SEQ. ID. NO: 9). A change in the plant height and internode length was observed compared with control plants.
摘要翻译：本发明涉及从异源和同源树种分离和表征新型扩展蛋白基因序列，并将这些新基因重新引入到树中以改变扩展蛋白水平。已经鉴定了六种新型基因。使用黄瓜EX29序列（GenBank，登录号U30382，称为Cs-EXP1）（SEQ ID NO：9）也已经转化了桉树。与对照植物相比，观察到植物高度和节间长度的变化。

6. 发明授权

US5990283A Proteins catalyzing the extension of plant cell walls 失效
标题翻译：催化植物细胞壁延伸的蛋白质
公开(公告)号：US5990283A
公开(公告)日：1999-11-23
申请号：US834327
申请日：1997-04-15
申请人： Daniel J. Cosgrove , Simon McQueen-Mason
发明人： Daniel J. Cosgrove , Simon McQueen-Mason
IPC分类号： C07K14/415 , C12N9/42 , C12N15/82 , C07K15/00
CPC分类号： C12N9/2437 , C07K14/415 , C12N15/8261
摘要： Plant cell expansion is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wall loosening" enzymes. By employing a reconstitution approach, we initially found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicots and monocots, but was less effective on grass coleoptile walls. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD, as measured by SDS-PAGE, associated with such activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall We proposed the name "expansins" for this class of proteins. Expansins have been isolated from various plant sources including oat, cucumber, broccoli, celery, tomato, cotton, cabbage, and corn, and also from snail and its feces. These proteins weaken the intermolecular bonds between plant wall polysaccharides. They decrease the mechanical strength of commercial products made from polysaccharides, such as paper, and therefore present a novel approach in developing new technologies in industries which make use of such polysaccharides, such as in the paper industry, in the applications of polysaccharide gums and related products. These proteins moreover present a novel approach in the control of plant growth.
摘要翻译：植物细胞膨胀受壁松弛和产量的调节，这被认为是由难以捉摸的“壁松动”酶催化的。通过采用重组方法，我们最初发现，生长黄瓜幼苗细胞壁的粗蛋白提取物具有诱导分离的细胞壁延伸的能力。该活动仅限于茎的生长区域，并且可以诱导来自各种双子叶植物和单子叶植物的分离的细胞壁的延伸，但是对于草胚芽鞘壁效果较差。活性壁提取物的顺序HPLC分级分离显示分子量为29和30kD的蛋白质，通过SDS-PAGE测量，与这种活性相关。每种蛋白质本身可以诱导壁延伸，而不会有可检测到的壁的水解破坏。我们提出了这类蛋白质的名称“膨胀物”。膨胀体已经从各种植物来源分离，包括燕麦，黄瓜，西兰花，芹菜，番茄，棉花，卷心菜和玉米，以及蜗牛及其粪便。这些蛋白质削弱植物壁多糖之间的分子间键。它们降低了由多糖制成的商业产品的机械强度，如纸张，因此在开发利用这种多糖的行业中开发新技术的新方法，如造纸工业，多糖胶和相关的应用产品。这些蛋白质还提供了一种控制植物生长的新方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式