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1. 发明授权

US10982191B2 Engineered mammalian cells for production of recombinant proteins 有权
公开(公告)号：US10982191B2
公开(公告)日：2021-04-20
申请号：US16095975
申请日：2017-04-25
申请人： DANMARKS TEKNISKE UNIVERSITET , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： Bjørn Gunnar Voldborg , Lasse Ebdrup Pedersen , Bernhard Palsson
IPC分类号： C12N5/071 , C12N9/64 , C12N15/113 , C12N15/62 , C12N15/79
摘要： The present invention relates to mammalian cells modified to provide for improved expression of a recombinant protein of interest. In particular, the invention relates to CHO cells and other host cells in which the expression of one or more endogenous secreted proteins has been disrupted, as well as to the preparation, identification and use of such cells in the production of recombinant proteins.

2. 发明申请

US20150011400A1 Bacterial Metastructure and Methods of Use 审中-公开
标题翻译：细菌结构和使用方法
公开(公告)号：US20150011400A1
公开(公告)日：2015-01-08
申请号：US14356588
申请日：2012-11-09
申请人： The Regents of the University of California
发明人： Bernhard Palsson , Byung-kwan Cho
IPC分类号： G06F19/12 , C12N1/20 , G06F19/22 , G06F19/18
CPC分类号： G16B5/00 , C12N1/20 , G16B20/00 , G16B30/00
摘要： Although metabolic networks have been reconstructed on a genome-scale, the corresponding reconstruction and integration of governing transcriptional regulatory networks has not been fully achieved. Here such an integrated network was constructed for amino acid metabolism in Escherichia coli. Analysis of ChlP-chip and gene expression data for the transcription factors ArgR, Lrp, and TrpR showed that 19/20 amino acid biosynthetic pathways are either directly or indirectly controlled by these regulators. Classifying the regulated genes into three functional categories of transport, biosynthesis, and metabolism leads to elucidation of regulatory motifs constituting the integrated network's basic building blocks. The regulatory logic of these motifs was determined based on the relationships between transcription factor binding and changes in transcript levels in response to exogenous amino acids. Remarkably, the resulting logic shows how amino acids are differentiated as signaling and nutrient molecules. This reveals the overarching regulatory principles of the amino acid stimulon.
摘要翻译：虽然代谢网络已经在基因组规模上重建，但相应的重建和整合调控转录调控网络尚未完全实现。在这里，构建了大肠杆菌中氨基酸代谢的综合网络。 ChlP芯片和转录因子ArgR，Lrp和TrpR的基因表达数据的分析显示，19/20个氨基酸生物合成途径直接或间接受这些调节因子控制。将调控的基因分为运输，生物合成和代谢三个功能类别，导致构成整合网络基本构件的调节基序的阐明。这些基序的调控逻辑是基于转录因子结合与转录因子对外源氨基酸的响应变化之间的关系而确定的。值得注意的是，所得到的逻辑表明氨基酸如何被分化为信号传导和营养分子。这揭示了氨基酸刺激素的总体监管原则。

3. 发明申请

US20150376676A1 METHOD TO GENERATE NOVEL BIOACTIVE MOLECULES 审中-公开
标题翻译：生成新生物活性分子的方法
公开(公告)号：US20150376676A1
公开(公告)日：2015-12-31
申请号：US14789871
申请日：2015-07-01
申请人： The Regents of the University of California
发明人： Bernhard Palsson , Pep Charusanti
IPC分类号： C12Q1/18
CPC分类号： C12Q1/18 , C12N1/20 , C12P1/06 , C12P39/00 , C12R1/465
摘要： The present invention describes a method to generate new chemical entities (NCEs) that have well-defined activities such as, but not limited to, anti-bacterial, antifungal and anthelmintic effects. The NCEs are generated through adaptive evolution of one microbe (the producer) against another organism or cell type (the target). The producer is made to compete against the target over time by co-culturing the two together and serially passing the producer organism until the producer adaptively evolves by synthesizing an NCE(s) that inhibits growth of or kills the target. The molecular structure of the chemical entity (or entities) is then elucidated using tools from genomics, molecular biology, computational biology, analytical chemistry, organic chemistry and related fields.
摘要翻译：本发明描述了一种产生新的化学实体（NCE）的方法，其具有明确的活性，例如但不限于抗细菌，抗真菌和驱虫效应。 NCEs通过一个微生物（生产者）对另一个生物或细胞类型（目标）的适应性演化产生。生产者通过共同培养两者并使生产者有机体连续通过生产者直到生产者通过合成抑制生长或杀死目标的NCE来自适应地演变，从而与目标进行竞争。然后使用基因组学，分子生物学，计算生物学，分析化学，有机化学和相关领域的工具阐明化学实体（或实体）的分子结构。

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