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    • 10. 发明授权
    • Reverse progeny mapping
    • 反向后代映射
    • US07860658B2
    • 2010-12-28
    • US11848800
    • 2007-08-31
    • Robert Helene Ghislain DirksJohannes Wilhelmus Schut
    • Robert Helene Ghislain DirksJohannes Wilhelmus Schut
    • G06F7/00
    • A01H1/04A01H1/00
    • Provided is a method for mapping traits in organisms, in particular in plants. The method comprises a) providing a population of SDR-0 organisms, in particular plants, that each arise from one member of a population of unreduced cells resulting from second division restitution, in particular a population of unreduced spores; b) producing SDR-1 progeny populations of each of these SDR-0 organisms; c) phenotyping the SDR-1 progeny populations to identify segregating traits within each SDR-1 progeny population; d) if segregating progeny are present in a SDR-1 progeny population, genotyping the corresponding SDR-0 organism and comparing the genotype thereof with the genotype of the other SDR-0 organisms to identify heterozygous chromosomal regions associated with the occurrence of the segregating trait identified in the SDR-1 progeny population.
    • 提供了一种用于在生物体,特别是植物中测定性状的方法。 该方法包括:a)提供一群SDR-0生物体,特别是植物,它们分别由二分之一归还的未减少细胞群体的一个成员,特别是未减少的孢子群体产生; b)生产这些SDR-0生物体的SDR-1后代群体; c)对SDR-1后代群体进行表型鉴定,以确定每个SDR-1后代群体内的分离性状; d)如果分离后代存在于SDR-1后代群体中,对相应的SDR-0生物进行基因分型,并将其基因型与其他SDR-0生物体的基因型进行比较,以鉴定与分离性状发生相关的杂合染色体区 在SDR-1后代人群中确定。