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1. 发明授权

US5958739A Mutant .alpha.-amylase 失效
标题翻译：突变型α-淀粉酶
公开(公告)号：US5958739A
公开(公告)日：1999-09-28
申请号：US704706
申请日：1997-02-20
申请人： Colin Mitchinson , Carol Requadt , Traci Ropp , Leif P. Solheim , Christopher Ringer , Anthony Day
发明人： Colin Mitchinson , Carol Requadt , Traci Ropp , Leif P. Solheim , Christopher Ringer , Anthony Day
IPC分类号： C12N9/28 , C12P19/14 , C07H21/04 , C12N9/26
CPC分类号： C12N9/2417 , C12Y302/01001
摘要： Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.
摘要翻译： PCT No.PCT / US96 / 09089第 371日期1997年2月20日 102（e）1997年2月20日PCT PCT 1996年6月6日PCT公布。公开号WO96 / 39528 日期：1996年12月19日公开了一种或多种天冬酰胺残基被不同氨基酸取代或缺失的新型α-淀粉酶。公开的α-淀粉酶显示改变或改进的低pH淀粉水解性能，稳定性和活性谱。

2. 发明授权

US5849549A Oxidatively stable alpha-amylase 失效
标题翻译：氧化稳定的α-淀粉酶
公开(公告)号：US5849549A
公开(公告)日：1998-12-15
申请号：US468698
申请日：1995-06-06
申请人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
发明人： Christopher C. Barnett , Leif P. Solheim , Colin Mitchinson , Scott D. Power , Carol A. Requadt
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N5/10 , C12N9/26 , C12N9/28 , C12N15/56 , C12N15/75 , C12P19/14
CPC分类号： C12N9/2417 , C11D3/386 , C12N15/75 , C12P19/14
摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
摘要翻译：公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶前体α-淀粉酶的序列。与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

3. 发明授权

US5652127A Method for liquefying starch 失效
标题翻译：液化淀粉的方法
公开(公告)号：US5652127A
公开(公告)日：1997-07-29
申请号：US459984
申请日：1995-06-02
申请人： Colin Mitchinson , Leif P. Solheim
发明人： Colin Mitchinson , Leif P. Solheim
IPC分类号： C08B30/12 , C12P19/14 , C12M9/24 , C12M9/26
CPC分类号： C12P19/14 , C08B30/12
摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.
摘要翻译：根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠，磷酸盐或其混合物。

4. 发明授权

US5756714A Method for liquefying starch 失效
标题翻译：液化淀粉的方法
公开(公告)号：US5756714A
公开(公告)日：1998-05-26
申请号：US411038
申请日：1995-03-27
申请人： Richard L. Antrim , Colin Mitchinson , Leif P. Solheim
发明人： Richard L. Antrim , Colin Mitchinson , Leif P. Solheim
IPC分类号： C08B30/12 , C12P19/14 , A23J1/14 , A23L1/211
CPC分类号： C08B30/12 , C12P19/14
摘要： According to the invention a method is provided for liquefying starch comprising the steps of treating the starch prior to or simultaneously with liquefying the starch to inactivate and/or remove the enzyme inhibiting composition present in the starch and form treated starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Effective means to treat the starch include the addition of a phytate degrading enzyme and heat treatment, optionally followed by filtration or centrifugation, of granular starch or a starch solution.
摘要翻译：根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时处理淀粉以灭活和/或除去淀粉中存在的酶抑制组合物并形成经处理的淀粉; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。处理淀粉的有效方法包括加入植酸盐降解酶和热处理，任选地随后过滤或离心淀粉或淀粉溶液。

5. 发明授权

US06184002B2 Method for liquefying starch 失效
标题翻译：液化淀粉的方法
公开(公告)号：US06184002B2
公开(公告)日：2001-02-06
申请号：US08952225
申请日：1998-01-27
申请人： Colin Mitchinson , Leif P. Solheim
发明人： Colin Mitchinson , Leif P. Solheim
IPC分类号： C12P1914
CPC分类号： C12P19/14 , C08B30/12
摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding &agr;-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.
摘要翻译：根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠，磷酸盐或其混合物。

6. 发明授权

US5736499A Mutant A-amylase 失效
标题翻译：突变型α-淀粉酶
公开(公告)号：US5736499A
公开(公告)日：1998-04-07
申请号：US468700
申请日：1995-06-06
申请人： Colin Mitchinson , Carol Ann Requadt , Traci Helen Ropp , Leif P. Solheim
发明人： Colin Mitchinson , Carol Ann Requadt , Traci Helen Ropp , Leif P. Solheim
IPC分类号： C12N15/00 , C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56
CPC分类号： C12N9/2417 , C11D3/386
摘要： Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.
摘要翻译：公开了一种或多种天冬酰胺残基被不同氨基酸取代或缺失的新型α-淀粉酶。公开的α-淀粉酶显示改变或改进的低pH淀粉水解性能，稳定性和活性谱。

7. 发明授权

US07972832B2 Variant Hypocrea jecorina CBH1 cellulases 有权
标题翻译：变异型Hecocina jecorina CBH1纤维素酶
公开(公告)号：US07972832B2
公开(公告)日：2011-07-05
申请号：US10641678
申请日：2003-08-15
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
IPC分类号： C12N9/42 , C12N1/20 , C12P19/02 , C12P19/00 , C12P21/06 , C07K14/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译：本文描述的是H.jecorina CBH I，Cel7酶的变体。本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。

8. 发明申请

US20070173431A1 Novel variant hypocrea jercorina CBH1 cellulases 失效
标题翻译：新型变异细菌CBH1纤维素酶
公开(公告)号：US20070173431A1
公开(公告)日：2007-07-26
申请号：US11728219
申请日：2007-03-22
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12P19/02 , C12N9/42 , C12N1/21 , C12N15/74
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译：本文描述的是H.jecorina CBH I，Cel7酶的变体。本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。

9. 发明申请

US20050277172A1 Novel variant hypocrea jecorina CBH1cellulases 有权
标题翻译：新型变种hypocrea jecorina CBH1纤维素酶
公开(公告)号：US20050277172A1
公开(公告)日：2005-12-15
申请号：US10641678
申请日：2003-08-15
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
IPC分类号： C07H21/04 , C12N9/42 , C12N15/74
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译：本文描述的是H.jecorina CBH I，Cel7酶的变体。本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。

10. 发明授权

US08232080B2 Variant Hyprocrea jecorina CBH1 cellulases 有权
标题翻译：变异Hyprocrea jecorina CBH1纤维素酶
公开(公告)号：US08232080B2
公开(公告)日：2012-07-31
申请号：US13107702
申请日：2011-05-13
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
IPC分类号： C12P19/02 , C12P19/00 , C12P21/06 , C12N9/42 , C12N1/20 , G01N33/53 , C07H21/04
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译：本文描述的是H.jecorina CBH I，Cel7酶的变体。本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。

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