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1. 发明授权

US08956840B2 Engineered ketoreductase polypeptides 有权
标题翻译：工程化酮还原酶多肽
公开(公告)号：US08956840B2
公开(公告)日：2015-02-17
申请号：US13764596
申请日：2013-02-11
申请人： Codexis, Inc.
发明人： Emily Mundorff , Erik De Vries
IPC分类号： C12N9/00 , C12P7/02 , C12N9/04 , C12P17/14 , C12P41/00
CPC分类号： C12N9/0006 , C12N15/00 , C12N15/75 , C12P7/02 , C12P17/10 , C12P17/14 , C12P41/002 , C12Y101/01184 , Y02P20/52
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
摘要翻译：本发明提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶，其包括还原5 - （（4S）-2-氧代-4-苯基（1,3-恶唑烷-3-基））） - 1-（4-氟苯基）戊烷-1,5-二酮转化为（4S）-3 - [（5S）-5-（4-氟苯基）-5-羟基戊酰基] -4-苯基-1,3- 恶唑烷-2-酮。还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞和使用工程化酮还原酶合成中间体（4S）-3 - [（5S）-5-（4-氟苯基） -5-羟基戊酰基] -4-苯基-1,3-恶唑烷-2-酮在制备依泽替米贝的方法中。

2. 发明申请

US20130210098A1 ENGINEERED KETOREDUCTASE POLYPEPTIDES 有权
标题翻译：工程糖酸盐多糖
公开(公告)号：US20130210098A1
公开(公告)日：2013-08-15
申请号：US13764596
申请日：2013-02-11
申请人： CODEXIS, INC.
发明人： Emily Mundorff , Erik De Vries
IPC分类号： C12N9/00 , C12P7/02
CPC分类号： C12N9/0006 , C12N15/00 , C12N15/75 , C12P7/02 , C12P17/10 , C12P17/14 , C12P41/002 , C12Y101/01184 , Y02P20/52
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
摘要翻译：本发明提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶，其包括还原5 - （（4S）-2-氧代-4-苯基（1,3-恶唑烷-3-基））） - 1-（4-氟苯基）戊烷-1,5-二酮转化为（4S）-3 - [（5S）-5-（4-氟苯基）-5-羟基戊酰基] -4-苯基-1,3- 恶唑烷-2-酮。还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞和使用工程化酮还原酶合成中间体（4S）-3 - [（5S）-5-（4-氟苯基） -5-羟基戊酰基] -4-苯基-1,3-恶唑烷-2-酮在制备依泽替米贝的方法中。

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