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1. 发明授权

US07076092B2 High-throughput, dual probe biological assays based on single molecule detection 失效
标题翻译：基于单分子检测的高通量双探针生物测定
公开(公告)号：US07076092B2
公开(公告)日：2006-07-11
申请号：US10170876
申请日：2002-06-12
申请人： Christopher W. Hollars , Thomas R. Huser , Stephen M. Lane , Rodney L. Balhorn , Olgica Bakajin , Christopher Darrow , Joe H. Satcher, Jr.
发明人： Christopher W. Hollars , Thomas R. Huser , Stephen M. Lane , Rodney L. Balhorn , Olgica Bakajin , Christopher Darrow , Joe H. Satcher, Jr.
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , B01L3/5027 , G01N21/6428 , G01N21/6456 , G01N33/54353 , G01N33/582 , G01N2021/058 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441
摘要： A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 μm×100 μm×100 μm. A device using these methods would have 103–104 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μm3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (˜10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.
摘要翻译：通过双重荧光标记的探针分子的定位，灵敏度检测和识别单个靶分子的方法和装置。这可以通过将标签特定附着到具有约0.5mum×100mum×100mum的大小的表面或二维（2D）流动流体片材中来实现。使用这些方法的装置将具有比具有1mum 3以上的一维（1D）微流装置更高的产量10％以上。 >询问体积，并将首次允许在短时间（〜10分钟）内进行超低（飞摩尔）浓度的免疫和DNA测定。可以引入新的标记（例如金属或半导体纳米颗粒）的使用以进一步将灵敏度扩展到极限范围内。

2. 发明授权

US5461907A Imaging, cutting, and collecting instrument and method 失效
标题翻译：成像，切割和收集仪器和方法
公开(公告)号：US5461907A
公开(公告)日：1995-10-31
申请号：US35741
申请日：1993-03-23
申请人： Robert J. Tench , Wigbert J. Siekhaus , Mehdi Balooch , Rodney L. Balhorn , Michael J. Allen
发明人： Robert J. Tench , Wigbert J. Siekhaus , Mehdi Balooch , Rodney L. Balhorn , Michael J. Allen
IPC分类号： G01Q60/24 , G01Q60/38 , H01J3/14
CPC分类号： G01Q60/38 , B82Y35/00 , G01Q70/10 , Y10S977/856
摘要： Instrumentation and techniques to image small objects, such as but not limited to individual human chromosomes, with nanometer resolution, to cut-off identified parts of such objects, to move around and manipulate such cut-off parts on the substrate on which they are being imaged to predetermined locations on the substrate, and to remove the cut-off parts from the substrate. This is accomplished using an atomic force microscope (AFM) and by modification of the conventional cantilever stylus assembly of an AFM, such that plural cantilevers are used with either sharp-tips or knife-edges thereon. In addition, the invention can be utilized for measuring hardness of materials.
摘要翻译：用于将小物体（例如但不限于单个人类染色体）以纳米分辨率成像的仪器和技术，以截断所述物体的识别部分，以移动并操纵其正在其上的基底上的这种截止部分成像到基板上的预定位置，并从基板去除截止部分。这是使用原子力显微镜（AFM）和通过修改AFM的常规悬臂测针组件来实现的，使得在其上使用尖锐尖端或刀刃的多个悬臂。此外，本发明可用于测量材料的硬度。

3. 发明授权

US4331660A Fish egg fungicide 失效
标题翻译：鱼蛋杀菌剂
公开(公告)号：US4331660A
公开(公告)日：1982-05-25
申请号：US239395
申请日：1981-03-02
申请人： Rodney L. Balhorn
发明人： Rodney L. Balhorn
IPC分类号： A01N59/16
CPC分类号： A01N59/16
摘要： The control of fungus is a serious problem in the hatching of fish. Sodium dichromate, potassium dichromate and chromium trioxide are effective fungicides for the inhibition of fungal growth on fish eggs and young fry during hatching.
摘要翻译：真菌的控制是鱼类孵化中的一个严重问题。重铬酸钠，重铬酸钾和三氧化铬是有效的杀真菌剂，用于在孵化期间抑制鱼卵和幼苗的真菌生长。

4. 发明申请

US20130306549A1 NANOPOROUS MEMBRANE 审中-公开
标题翻译：纳米膜
公开(公告)号：US20130306549A1
公开(公告)日：2013-11-21
申请号：US13949082
申请日：2013-07-23
申请人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
发明人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
IPC分类号： B01D71/02 , B01D67/00
CPC分类号： B01D71/027 , B01D67/0058 , B01D67/0062 , B01D69/02 , B01D71/02 , B01D2323/24 , B01D2325/021 , B82Y30/00 , B82Y40/00
摘要： A nano porous membrane having a uniform array of nanopores etch-formed in a thin film structure (e.g. (100)-oriented single crystal silicon) having a predetermined thickness, by (a) using interferometric lithography to create an etch pattern comprising a plurality array of unit patterns having a predetermined width/diameter, (b) using the etch pattern to etch frustum-shaped cavities or pits in the thin film structure such that the dimension of the frustum floors of the cavities are substantially equal to a desired pore size based on the predetermined thickness of the thin film structure and the predetermined width/diameter of the unit patterns, and (c) removing the frustum floors at a boundary plane of the thin film structure to expose, open, and thereby create the nanopores substantially having the desired pore size.
摘要翻译：一种具有预定厚度的薄膜结构（例如（100）取向的单晶硅）中蚀刻形成的纳米孔的均匀阵列的纳米多孔膜，通过（a）使用干涉光刻技术产生包括多个阵列的蚀刻图案具有预定宽度/直径的单元图案，（b）使用蚀刻图案来蚀刻薄膜结构中的截头锥形空腔或凹坑，使得空腔的截头锥形地板的尺寸基本上等于基于期望的孔径尺寸在薄膜结构的预定厚度和单位图案的预定宽度/直径上，以及（c）在薄膜结构的边界平面处去除截头锥形地板以暴露，打开，从而产生基本上具有所需的孔径。

5. 发明申请

US20120037591A1 METHOD OF FABRICATING A SCALABLE NANOPOROUS MEMBRANE FILTER 有权
标题翻译：制备可扩展的纳米膜过滤器的方法
公开(公告)号：US20120037591A1
公开(公告)日：2012-02-16
申请号：US12856527
申请日：2010-08-13
申请人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
发明人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
IPC分类号： C23F1/00
CPC分类号： B01D71/027 , B01D67/0058 , B01D67/0062 , B01D69/02 , B01D71/02 , B01D2323/24 , B01D2325/021 , B82Y30/00 , B82Y40/00
摘要： A method of fabricating a nanoporous membrane filter having a uniform array of nanopores etch-formed in a thin film structure (e.g. (100)-oriented single crystal silicon) having a predetermined thickness, by (a) using interferometric lithography to create an etch pattern comprising a plurality array of unit patterns having a predetermined width/diameter, (b) using the etch pattern to etch frustum-shaped cavities or pits in the thin film structure such that the dimension of the frustum floors of the cavities are substantially equal to a desired pore size based on the predetermined thickness of the thin film structure and the predetermined width/diameter of the unit patterns, and (c) removing the frustum floors at a boundary plane of the thin film structure to expose, open, and thereby create the nanopores substantially having the desired pore size.
摘要翻译：一种通过（a）使用干涉光刻技术制造具有预定厚度的薄膜结构（例如（100）取向的单晶硅）中蚀刻形成的纳米孔的均匀阵列的纳米多孔膜滤光片的方法，以产生蚀刻图案包括具有预定宽度/直径的多个单元图案阵列，（b）使用蚀刻图案蚀刻薄膜结构中的截头锥形腔或凹坑，使得空腔的截头锥形地板的尺寸基本上等于基于薄膜结构的预定厚度和单位图案的预定宽度/直径所需的孔径，以及（c）在薄膜结构的边界平面处去除截头锥形地板以暴露，打开，从而形成基本上具有所需孔径的纳米孔。

6. 发明授权

US09884070B2 Selective high-affinity polydentate ligands and methods of making such 有权
公开(公告)号：US09884070B2
公开(公告)日：2018-02-06
申请号：US12988801
申请日：2009-04-21
申请人： Sally J. Denardo , Gerald L. Denardo , Rodney L. Balhorn
发明人： Sally J. Denardo , Gerald L. Denardo , Rodney L. Balhorn
IPC分类号： A61K31/675 , A61K47/48
CPC分类号： A61K31/675 , A61K47/545 , A61K47/55 , A61K47/60 , A61K47/64 , A61K47/645
摘要： This invention provides novel polydentate selective high affinity ligands (SHALs) that can be used in a variety of applications in a manner analogous to the use of antibodies. SHALs typically comprise a multiplicity of ligands that each bind different region son the target molecule. The ligands are joined directly or through a linker thereby forming a polydentate moiety that typically binds the target molecule with high selectivity and avidity.

7. 发明授权

US08512588B2 Method of fabricating a scalable nanoporous membrane filter 有权
标题翻译：制造可扩展纳米多孔膜过滤器的方法
公开(公告)号：US08512588B2
公开(公告)日：2013-08-20
申请号：US12856527
申请日：2010-08-13
申请人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
发明人： Joseph W. Tringe , Rodney L. Balhorn , Saleem Zaidi
IPC分类号： B44C1/22
CPC分类号： B01D71/027 , B01D67/0058 , B01D67/0062 , B01D69/02 , B01D71/02 , B01D2323/24 , B01D2325/021 , B82Y30/00 , B82Y40/00
摘要： A method of fabricating a nanoporous membrane filter having a uniform array of nanopores etch-formed in a thin film structure (e.g. (100)-oriented single crystal silicon) having a predetermined thickness, by (a) using interferometric lithography to create an etch pattern comprising a plurality array of unit patterns having a predetermined width/diameter, (b) using the etch pattern to etch frustum-shaped cavities or pits in the thin film structure such that the dimension of the frustum floors of the cavities are substantially equal to a desired pore size based on the predetermined thickness of the thin film structure and the predetermined width/diameter of the unit patterns, and (c) removing the frustum floors at a boundary plane of the thin film structure to expose, open, and thereby create the nanopores substantially having the desired pore size.
摘要翻译：一种通过（a）使用干涉光刻技术制造具有预定厚度的薄膜结构（例如（100）取向的单晶硅）中蚀刻形成的纳米孔的均匀阵列的纳米多孔膜滤光片的方法，以产生蚀刻图案包括具有预定宽度/直径的多个单元图案阵列，（b）使用蚀刻图案蚀刻薄膜结构中的截头锥形腔或凹坑，使得空腔的截头锥形地板的尺寸基本上等于基于薄膜结构的预定厚度和单位图案的预定宽度/直径所需的孔径，以及（c）在薄膜结构的边界平面处去除截头锥形地板以暴露，打开，从而形成基本上具有所需孔径的纳米孔。

8. 发明申请

US20110144065A1 SELECTIVE HIGH-AFFINITY POLYDENTATE LIGANDS AND METHODS OF MAKING SUCH 有权
标题翻译：选择性高亲和力配体及其制备方法
公开(公告)号：US20110144065A1
公开(公告)日：2011-06-16
申请号：US12988801
申请日：2009-04-21
申请人： Sally J. Denardo , Gerald L. Denardo , Rodney L. Balhorn
发明人： Sally J. Denardo , Gerald L. Denardo , Rodney L. Balhorn
IPC分类号： A61K31/655 , C07C245/08 , C12Q1/02 , A61P35/00
CPC分类号： A61K31/675 , A61K47/545 , A61K47/55 , A61K47/60 , A61K47/64 , A61K47/645
摘要： This invention provides novel polydentate selective high affinity ligands (SHALs) that can be used in a variety of applications in a manner analogous to the use of antibodies. SHALs typically comprise a multiplicity of ligands that each bind different region son the target molecule. The ligands are joined directly or through a linker thereby forming a polydentate moiety that typically binds the target molecule with high selectivity and avidity.
摘要翻译：本发明提供了新颖的多齿选择性高亲和力配体（SHAL），其可以以与使用抗体类似的方式用于多种应用。 SHAL通常包含多个配体，其各自结合不同区域的靶分子。配体直接或通过接头连接，从而形成通常以高选择性和亲合力结合目标分子的多齿部分。

9. 发明授权

US06420112B2 DNA attachment to support structures 失效
标题翻译： DNA附着到支持结构
公开(公告)号：US06420112B2
公开(公告)日：2002-07-16
申请号：US09359147
申请日：1999-07-21
申请人： Rodney L. Balhorn , Christopher H. Barry
发明人： Rodney L. Balhorn , Christopher H. Barry
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6834 , Y10S977/924 , Y10T436/143333 , C12Q2565/102 , C12Q2563/131 , C12Q2521/131
摘要： Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).
摘要翻译：显微镜珠或其他结构使用末端转移酶与核酸（DNA）连接。转移酶将标记的双脱氧核苷酸碱基添加到DNA的线性链的末端。标记，例如抗原地高辛和生物素结合抗体化合物或其它合适的互补配体，其结合到微珠或其它支持结构上。该方法不需要合成寡核苷酸探针。即使DNA具有未知序列，具有平末端，或者是非常大的片段（例如> 500千碱基对），该方法可用于标记或标记DNA。

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