专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

US20080318222A1 Cell Based Methods And Systems For The Identification Of Rna Regulatory Sequences And Compounds That Modulate Their Functions 有权
标题翻译：基于细胞的方法和系统用于鉴定调节其功能的Rna调节序列和化合物
公开(公告)号：US20080318222A1
公开(公告)日：2008-12-25
申请号：US11813027
申请日：2005-12-27
申请人： Matthew C. Pelligrini , Christopher R. Trotta
发明人： Matthew C. Pelligrini , Christopher R. Trotta
IPC分类号： C12Q1/68 , C12N5/10
CPC分类号： G01N33/5008 , C12N15/1086 , C12Q1/68
摘要： The present invention is directed to an in vivo method for screening for and/or identifying RNA regulatory sequences involved in translational control. The method includes: culturing cells including: (i) a translational system; (ii) a reporter mRNA; and (iii) an RNA test sequence under suitable conditions for translation of the reporter mRNA, wherein the RNA test sequence is not attached to the reporter mRNA. The method also includes measuring the effect of the test sequence on the translation of the reporter mRNA, wherein a test sequence that modifies the translation of the reporter mRNA includes an RNA regulatory element. The invention also provides methods and systems for screening for and/or identifying test compounds that modulate the regulatory activity of an RNA regulatory sequence. This method includes culturing cells including (i) a translation system; (ii) a reporter mRNA; and (iii) an RNA regulatory sequence in the presence of at least one test compound, wherein the RNA regulatory sequence is not attached to the reporter mRNA. The ability of the test compound to affect the interaction between the RNA regulatory sequence and at least one component of the translation system is sought to be determined. The method further includes monitoring for the effect of the test compound on the this interaction, wherein a compound that modifies this interaction is a drug candidate.
摘要翻译：本发明涉及用于筛选和/或鉴定涉及翻译控制的RNA调节序列的体内方法。该方法包括：培养细胞，包括：（i）翻译系统; （ii）报告mRNA; 和（iii）在用于翻译报告mRNA的合适条件下的RNA测试序列，其中RNA测试序列不与报道mRNA连接。该方法还包括测量测试序列对报告mRNA的翻译的影响，其中修饰报道mRNA的翻译的测试序列包括RNA调节元件。本发明还提供用于筛选和/或鉴定调节RNA调节序列的调节活性的测试化合物的方法和系统。该方法包括培养细胞，包括（i）翻译系统; （ii）报告mRNA; 和（iii）在至少一种测试化合物存在下的RNA调节序列，其中所述RNA调节序列不与报道mRNA连接。试图确定测试化合物影响RNA调节序列与翻译系统的至少一个组分之间的相互作用的能力。该方法还包括监测测试化合物对该相互作用的影响，其中修饰该相互作用的化合物是候选药物。

IPRDB

热门服务

关于我们

友情链接

联系方式