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    • 5. 发明授权
    • Two-phase optical assay
    • 两相光学测定
    • US5674699A
    • 1997-10-07
    • US361832
    • 1994-12-22
    • Alexander SaundersMichael Allan Zarowitz
    • Alexander SaundersMichael Allan Zarowitz
    • G01N33/543G01N33/58G01N33/72G01N33/548G01N33/573
    • G01N33/582G01N33/54313G01N33/721G01N33/723
    • A method is provided for measuring an analyte in a sample comprising adding substantially transparent particles to a sample in solution or suspension, said particles having an affinity for said analyte; fractionating the particles from the solution or suspension to form a particle-rich fraction and a substantially particle-free fraction; optically reading the particle-rich fraction at a first and a second wavelength; optically reading the substantially particle-free fraction at at least the first wavelength; and correlating the readings through the particle-rich fraction and the substantially particle-free fraction of the sample, with similar measurements in a particle-containing "blank" to obtain a quantitative determination of the analyte originally present in the sample.
    • 提供了一种用于测量样品中的分析物的方法,包括在溶液或悬浮液中向样品中加入基本上透明的颗粒,所述颗粒对所述分析物具有亲和力; 从溶液或悬浮液中分离颗粒以形成富含颗粒的级分和基本上无颗粒的级分; 在第一和第二波长下光学地读取富含粒子的级分; 在至少第一波长下光学地读取基本上无颗粒的级分; 并通过富含颗粒的部分和基本上无颗粒的样品部分将读数与含有颗粒的“空白”中的相似测量值相关联,以获得样品中最初存在的分析物的定量测定。