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1. 发明申请

US20090298085A1 Detection of Extracellular Tumor-Associated Nucleic Acid in Blood Plasma or Serum Using Nucleic Acid Amplification Assays 有权
标题翻译：使用核酸扩增测定检测血浆或血清中细胞外肿瘤相关核酸
公开(公告)号：US20090298085A1
公开(公告)日：2009-12-03
申请号：US12534578
申请日：2009-08-03
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

2. 发明授权

US06939675B2 Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays 失效
标题翻译：使用核酸扩增测定法检测血浆或血清中的细胞外肿瘤相关核酸
公开(公告)号：US06939675B2
公开(公告)日：2005-09-06
申请号：US10298816
申请日：2002-11-18
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C07K14/47 , C07K14/82 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

3. 发明授权

US06521409B1 Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays 有权
标题翻译：使用核酸扩增测定法检测血浆或血清中的细胞外肿瘤相关核酸
公开(公告)号：US06521409B1
公开(公告)日：2003-02-18
申请号：US09642952
申请日：2000-08-21
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q168
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

4. 发明申请

US20080233569A1 Detection of Extracellular Tumor-Associated Nucleic Acid in Blood Plasma or Serum Using Nucleic Acid Amplification Assays 失效
标题翻译：使用核酸扩增测定检测血浆或血清中细胞外肿瘤相关核酸
公开(公告)号：US20080233569A1
公开(公告)日：2008-09-25
申请号：US11739494
申请日：2007-04-24
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

5. 发明授权

US6156504A Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays 失效
标题翻译：使用核酸扩增测定法检测血浆或血清中的细胞外肿瘤相关核酸
公开(公告)号：US6156504A
公开(公告)日：2000-12-05
申请号：US818058
申请日：1997-03-14
申请人： Christopher D. Gocke , Floyd A. Benko , Michael S. Kopreski
发明人： Christopher D. Gocke , Floyd A. Benko , Michael S. Kopreski
IPC分类号： C07K14/47 , C07K14/82 , C12Q1/68 , C12Q1/70 , C07H21/04 , C12P19/34
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C07K2319/00 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood, using DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or animals that contain a mutation associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及从突变致癌基因或其他肿瘤相关DNA衍生的核酸的检测，以及使用DNA提取后检测和监测血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法通过用于突变体DNA的富集或不富集的核酸扩增。特别地，本发明涉及通过检测，检测，鉴定或监测包含与赘生物相关的突变的人或动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清部分的肿瘤。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

6. 发明授权

US07569350B2 Methods for selecting cancer vaccine therapies using extracellular DNA 失效
标题翻译：使用细胞外DNA选择癌症疫苗疗法的方法
公开(公告)号：US07569350B2
公开(公告)日：2009-08-04
申请号：US11739494
申请日：2007-04-24
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

7. 发明申请

US20080233568A1 Detection of Extracellular Tumor-Associated Nucleic Acid in Blood Plasma or Serum Using Nucleic Acid Amplification Assays 失效
公开(公告)号：US20080233568A1
公开(公告)日：2008-09-25
申请号：US11739477
申请日：2007-04-24
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.

8. 发明授权

US07399592B2 Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays 有权
标题翻译：使用核酸扩增测定法检测血浆或血清中的细胞外肿瘤相关核酸
公开(公告)号：US07399592B2
公开(公告)日：2008-07-15
申请号：US11421492
申请日：2006-06-01
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or maligant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变的，检测，鉴定或监测包含与肿瘤相关的突变的人或其他动物中的良性，恶变或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

9. 发明授权

US07569349B2 Methods for selecting monoclonal antibody therapies using extracellular DNA 失效
标题翻译：使用细胞外DNA选择单克隆抗体疗法的方法
公开(公告)号：US07569349B2
公开(公告)日：2009-08-04
申请号：US11739477
申请日：2007-04-24
申请人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
发明人： Christopher D. Gocke , Michael S. Kopreski , Floyd A. Benko
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07K14/82 , C07K14/47 , C07K14/4746 , C07K2319/00 , C12Q1/683 , C12Q1/686 , C12Q1/6865 , C12Q1/6886 , C12Q1/708 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2600/16 , C12Q2535/125
摘要： This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.
摘要翻译：本发明涉及在与肿瘤或增生性疾病相关的人或动物血液的血浆或血清部分中检测特异性细胞外核酸。具体地，本发明涉及来自突变致癌基因或其他肿瘤相关DNA的核酸的检测，以及通过使用快速DNA检测和监测在血浆或血清部分中发现的细胞外突变致癌基因或肿瘤相关DNA的方法提取后进行核酸扩增，扩增或不扩增突变DNA。特别地，本发明涉及通过检测突变体的检测，鉴定或监测包含与赘生物相关的突变的人或其他动物中的良性，恶化前或恶性肿瘤的存在，进展或临床状态血浆或血清级分中的肿瘤核酸。本发明允许检测被认为具有肿瘤或增殖性疾病的人或其他动物的血清或血浆中的细胞外，肿瘤相关核酸，或者在没有任何先前的肿瘤或增殖性疾病的历史或诊断的个体中。本发明提供了检测源自已知与肿瘤形成相关的遗传序列的细胞外核酸的能力，例如癌基因，以及先前未被认识为与肿瘤或增殖性疾病相关的遗传序列。因此，本发明提供了用于早期鉴定结直肠，胰腺，肺，乳腺，膀胱，卵巢，淋巴瘤和携带DNA的肿瘤相关突变的所有其他恶性肿瘤的方法以及用于监测人和其他动物中的癌症和其他肿瘤性疾病的方法。

10. 发明授权

US07968317B2 Detection of 5T4 RNA in plasma and serum 有权
标题翻译：血浆和血清中5T4 RNA的检测
公开(公告)号：US07968317B2
公开(公告)日：2011-06-28
申请号：US11415968
申请日：2006-05-02
申请人： Michael S. Kopreski , Christopher D. Gocke
发明人： Michael S. Kopreski , Christopher D. Gocke
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/158
摘要： This invention provides methods for detecting the presence of malignant or premalignant cells, or trophoblastic cells in a human wherein the malignant, premalignant or trophoblastic cells express 5T4. The methods of the invention detect 5T4 RNA in blood, blood plasma, serum, and other bodily fluids. The inventive methods are useful for detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease, and for the detection and evaluation of placental tissue in pregnant women.
摘要翻译：本发明提供了用于检测人类恶性或癌前细胞或滋养细胞的存在的方法，其中恶性，恶化前或滋养细胞表达5T4。本发明的方法检测血液，血浆，血清和其他体液中的5T4 RNA。本发明的方法可用于检测，诊断，监测，治疗或评估肿瘤疾病，以及孕妇胎盘组织的检测和评价。

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