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1. 发明授权

US5972663A Microorganisms and processes for the fermentative preparation of L-cysteine, L-cystine, N-acetylserine or thiazolidine derivatives 失效
标题翻译： L-半胱氨酸，L-胱氨酸，N-乙酰丝氨酸或噻唑烷衍生物的发酵制剂的微生物和方法
公开(公告)号：US5972663A
公开(公告)日：1999-10-26
申请号：US97759
申请日：1998-06-16
申请人： Christoph Winterhalter , Walfred Leinfelder
发明人： Christoph Winterhalter , Walfred Leinfelder
IPC分类号： C12N15/09 , C07K14/245 , C12N1/21 , C12N15/31 , C12P13/12 , C12R1/19 , C12N1/20 , C12N15/67
CPC分类号： C12P13/12 , C07K14/245
摘要： Microorganisms and processes for the fermentative preparation of L-cysteine, L-cystine, N-acetylserine or thiazolidine derivatives. The microorganism strain which is suitable for the fermentative preparation of L-cysteine, L-cystine, N-acetylserine and/or thiazolidine derivatives, overexpresses at least one gene which encodes a protein which is directly suitable for secreting antibiotics, or other substances which are toxic for the microorganism, out of the cell.
摘要翻译： L-半胱氨酸，L-胱氨酸，N-乙酰丝氨酸或噻唑烷衍生物的发酵制剂的微生物和方法。适用于L-半胱氨酸，L-胱氨酸，N-乙酰丝氨酸和/或噻唑烷衍生物的发酵制剂的微生物菌株过表达至少一种编码直接适于分泌抗生素的蛋白质的基因或其它对微生物有毒，离开细胞。

2. 发明授权

US06218168B1 Process for preparing O-acetylserine, L-cysteine and L-cysteine-related products 失效
标题翻译： O-乙酰丝氨酸，L-半胱氨酸和L-半胱氨酸相关产物的制备方法
公开(公告)号：US06218168B1
公开(公告)日：2001-04-17
申请号：US09065104
申请日：1998-05-13
申请人： Walfred Leinfelder , Peter Heinrich
发明人： Walfred Leinfelder , Peter Heinrich
IPC分类号： C12N120
CPC分类号： C12N9/1029 , C12P13/12
摘要： A process is for preparing O-acetylserine, L-cysteine and sulphurous compounds derived therefrom using feedback-resistant serine acetyl transferases. In comparison with the wild-type enzyme, these serine acetyl transferases have reduced sensitivity to the inhibitor L-cysteine and a protein sequence which, in comparison with the wild-type enzyme, displays at least one mutation or deletion. The process has a mutation which lies in the sequence region of the amino acid in position 97 up to and including the amino acid in position 273 or the deletion lies in the carboxy terminal sequence region as from the amino acid in position 227, position 1 being the starter methionine of FIG. 5 (SEQ ID NO:1) and the mutation from Met to Ile in position 256 being excluded.
摘要翻译：一种方法是使用反馈抗性丝氨酸乙酰转移酶制备O-乙酰丝氨酸，L-半胱氨酸和由其衍生的含硫化合物。与野生型酶相比，这些丝氨酸乙酰转移酶对抑制剂L-半胱氨酸和蛋白质序列的敏感性降低，与野生型酶相比，显示至少一种突变或缺失。该方法具有位于第97位的氨基酸的序列区，直至包括位置273的氨基酸的氨基酸的序列区域或缺失位于羧基末端序列区域，如位置227的氨基酸位置1为图1的起始蛋氨酸 5（SEQ ID NO：1）和从位置256处的Met到Ile的突变被排除。

3. 发明授权

US06180373B2 Microorganisms for the production of tryptophan and process for the preparation thereof 失效
标题翻译：用于生产色氨酸的微生物及其制备方法
公开(公告)号：US06180373B2
公开(公告)日：2001-01-30
申请号：US08411760
申请日：1995-04-12
申请人： G{umlaut over (u)}nter Wich , Walfred Leinfelder , Keith Backman
发明人： G{umlaut over (u)}nter Wich , Walfred Leinfelder , Keith Backman
IPC分类号： C12P1322
CPC分类号： C12R1/15 , C12P13/22 , Y10S435/843 , Y10S435/849
摘要： A tryptophan producing strain of microorganism is selected from E. coli and Corynebacteria and is tryptophan feedback resistant and serine feedback resistant. The serine feedback resistance is by a mutation in a serA allele, where the mutated serA allele codes for a protein which has a Ki value for serine between 0.1 mM and 50 mM. The tryptophan feedback resistance is by a trpE allele which codes for a protein which has a Ki value for tryptophan between 0.1 mM and 20 mM. A process for preparing this microorganism and a process for using this microorganism are disclosed.
摘要翻译：微生物的色氨酸生产菌株选自大肠杆菌和棒杆菌，是色氨酸反馈抗性和丝氨酸反馈抗性。丝氨酸反馈电阻是通过serA等位基因中的突变，其中突变的serA等位基因编码对于丝氨酸在0.1mM和50mM之间的Ki值的蛋白质。色氨酸反馈电阻是由trpE等位基因编码的蛋白质，其色氨酸的Ki值为0.1mM至20mM。公开了一种制备该微生物的方法和使用该微生物的方法。

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