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    • 2. 发明申请
    • FUNCTIONAL GENOMICS ASSAY FOR CHARACTERIZING PLURIPOTENT STEM CELL UTILITY AND SAFETY
    • 功能性基因测定用于表征多发性骨髓瘤细胞应用和安全性
    • US20130296183A1
    • 2013-11-07
    • US13822336
    • 2011-09-16
    • Kevin C. EgganAlexander MeissnerChristoph BockEvangelos KiskinisGriet Annie Frans Verstappen
    • Kevin C. EgganAlexander MeissnerChristoph BockEvangelos KiskinisGriet Annie Frans Verstappen
    • C12N15/10
    • C12N15/1072C12Q1/6881C12Q2600/154C12Q2600/158
    • The present invention generally relates set of reference data or “scorecard” for a pluripotent stem cell, and methods, systems and kits to generate a scorecard for predicting the functionality and suitability of a pluripotent stem cell line for a desired use. In some aspects, a method for generating a scorecard comprises using at least 2 stem cell assays selected from: epigenetic profiling, differentiation assay and gene expression assay to predict the functionality and suitability of a pluripotent stem cell line for a desired use. In some embodiments, the scorecard reference data can be compared with the pluripotent stem cells data to effectively and accurately predict the utility of the pluripotent stem cell for a given application, as well as any to identify specific characteristics of the pluripotent stem cell line to determine their suitability for downstream applications, such as for example, their suitability for therapeutic use, drug screening and toxicity assays, differentiation into a desired cell lineage, and the like.
    • 本发明通常涉及多能干细胞的参考数据或“记分卡”,以及生成用于预测多能干细胞系用于期望用途的功能和适用性的记分卡的方法,系统和试剂盒。 在一些方面,用于产生记分卡的方法包括使用选自以下的至少2种干细胞测定:表观遗传学分析,分化测定和基因表达测定来预测多能干细胞系用于期望用途的功能性和适用性。 在一些实施方案中,记分卡参考数据可以与多能干细胞数据进行比较,以有效和准确地预测给定应用的多能干细胞的效用,以及任何鉴定多能干细胞系的特定特征以确定 它们对下游应用的适用性,例如其对治疗用途的适用性,药物筛选和毒性测定,分化成所需细胞谱系等。