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    • 1. 发明授权
    • Method for quantifying oxidative stress caused by different biological pathways
    • 用于量化不同生物学途径引起的氧化应激的方法
    • US07993859B2
    • 2011-08-09
    • US12832176
    • 2010-07-08
    • Christine Des RosiersJean-Francois LesgardsJean-Claude Tardif
    • Christine Des RosiersJean-Francois LesgardsJean-Claude Tardif
    • G01N1/00G01N33/53
    • G01N33/5091
    • The present invention relates to methods for detecting a oxidative stress in a biological sample, methods of determining a cumulative record of oxidative injury, and methods of diagnosing diseases of aging, such as cardiovascular diseases, based on the presence or absence of a biomarker or a component thereof. The present invention also relates to a kit for detecting oxidative stress in a biological sample comprising a stabilizing reactant and an antibody. In some embodiments of the invention, the biomarker of oxidative stress is selected from an aldehyde-protein adduct and an aldehyde metabolite-protein adduct, and the proposed method further comprises measuring separately for the same protein biomarkers of oxydative stress formed with different amino acids of the protein.
    • 本发明涉及用于检测生物样品中的氧化应激的方法,确定氧化损伤的累积记录的方法,以及基于生物标志物的存在或不存在的,诸如心血管疾病的老化疾病的诊断方法 其组分。 本发明还涉及用于检测包含稳定反应物和抗体的生物样品中的氧化应激的试剂盒。 在本发明的一些实施方案中,氧化应激的生物标志物选自醛 - 蛋白质加合物和醛代谢物 - 蛋​​白质加合物,并且所提出的方法还包括分别测定与不同氨基酸形成的氧化应激相同的蛋白质生物标志物 蛋白质。