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1. 发明授权

US5789174A Detection of periodontal pathogens including bacteroides forsythus, porphyromonas gingivalis, prevotilla intermedia and prevotella nigrescens 失效
标题翻译：检测牙周病原体，包括孢霉菌，牙龈卟啉单胞菌，中间早熟和黑曲霉
公开(公告)号：US5789174A
公开(公告)日：1998-08-04
申请号：US729447
申请日：1996-10-11
申请人： Christian Mouton , Emmanuelle Guillot , Christian Menard
发明人： Christian Mouton , Emmanuelle Guillot , Christian Menard
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/689
摘要： The present invention relates to probes and primers for the detection of periodontal pathogens such as Bacteroides forsythus, Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens. As well, the invention relates to rapid and sensitive methods for the detection and identification of periodontal pathogens from an oral sample taken from a patient. Diagnostic kits for the detection and identification of periodontal pathogens in an oral sample from a patient, are also disclosed.
摘要翻译：本发明涉及用于检测牙周病原体的探针和引物，例如拟杆菌属（Bacteroides forsythus），牙龈卟啉单胞菌（Porphyromonas gingivalis），中间型Prevlotella和Prevotella nigrescens。此外，本发明还涉及从患者口服样品中检测和鉴定牙周病原体的快速和灵敏的方法。还公开了用于在患者的口腔样品中检测和鉴定牙周病原体的诊断试剂盒。

2. 发明授权

US06808879B1 Means for qualitative and quantitative analysis of microbial populations potentially present in a sample 失效
标题翻译：用于定性和定量分析样品中潜在存在的微生物群体的方法
公开(公告)号：US06808879B1
公开(公告)日：2004-10-26
申请号：US09529217
申请日：2000-06-05
申请人： Emmanuelle Guillot , Vincent Urbain , Jacques Manem , Bruce E. Rittmann , David A. Stahl , Jodi Flax , Michaël Wagner
发明人： Emmanuelle Guillot , Vincent Urbain , Jacques Manem , Bruce E. Rittmann , David A. Stahl , Jodi Flax , Michaël Wagner
IPC分类号： C12P168
CPC分类号： C12Q1/689
摘要： This invention relates to means of qualitative and quantitative analysis of microbial populations potentially present in a sample. These means notably comprise the use of at least one RNA-targeted oligonucleotide probe for in situ hybridization in whole cells; followed by the extraction of those probes which have become hybridized by separation from their target and elution from the microbial cells; as well as the detection and measurement of said extracted probes.
摘要翻译：本发明涉及定性和定量分析样品中潜在存在的微生物种群的方法。这些意味着特别包括使用至少一种RNA靶向寡核苷酸探针在全细胞中进行原位杂交; 然后提取通过从其靶分离而从微生物细胞中分离而杂交的那些探针; 以及所述提取的探针的检测和测量。

3. 发明申请

US20080171314A1 Method for controlling the microbiological quality of an aqueous medium and kit therefor 审中-公开
标题翻译：用于控制水性介质的微生物质量的方法及其试剂盒
公开(公告)号：US20080171314A1
公开(公告)日：2008-07-17
申请号：US12010716
申请日：2008-01-29
申请人： Patricia Renaud , Emmanuelle Guillot , Claude Mabilat , Carole Vachon , Bruno Lacroix , Guy Vernet , Marie-Astrid Armand , Philippe Laffaire
发明人： Patricia Renaud , Emmanuelle Guillot , Claude Mabilat , Carole Vachon , Bruno Lacroix , Guy Vernet , Marie-Astrid Armand , Philippe Laffaire
IPC分类号： C12Q1/68 , C07H21/04 , C12Q1/70
CPC分类号： C12Q1/701 , C12Q1/689 , C12Q1/6893
摘要： Disclosed is a method for controlling the microbiological quality of an environmental aqueous medium, suspected of containing various micro-organisms, having the following steps: selecting a reference set, having at least three micro-organisms, representing jointly or separately a microbiological quality level; providing a microbiological detection kit, having at least three probes specifically and respectively identifying said three micro-organisms; after treating the medium to be analyzed, contacting said micro-organisms, or any fraction thereof derived from the medium to be analyzed therefrom, with said detection kit, whereby a multiple determination of the micro-organisms is carried out, the determination representing the microbiological quality level of the medium.
摘要翻译：公开了一种用于控制怀疑含有各种微生物的环境水性介质的微生物质量的方法，具有以下步骤：选择具有至少三种微生物的参考文献，共同或分别表示微生物质量水平; 提供微生物检测试剂盒，具有特异性和分别鉴定所述三种微生物的至少三种探针; 在处理要分析的培养基之后，将所述微生物或其衍生自待分析培养基的任何部分与所述检测试剂盒接触，由此进行微生物的多重测定，所述微生物质量水平的介质。

4. 发明授权

US07179636B2 Preparing a sample to be analyzed from a sample with very large volume 有权
标题翻译：准备要从具有非常大体积的样品分析样品
公开(公告)号：US07179636B2
公开(公告)日：2007-02-20
申请号：US10297769
申请日：2001-06-08
申请人： Emmanuelle Guillot , Laurence Durand-Bourlier , Stephane Bulteau
发明人： Emmanuelle Guillot , Laurence Durand-Bourlier , Stephane Bulteau
IPC分类号： C12N1/02
CPC分类号： B01D61/145 , B01D61/18 , B01D63/02 , C12M25/10 , C12M29/16 , G01N1/4077 , G01N2001/4016 , G01N2001/4088
摘要： The invention concerns the use of filtering means comprising a housing (2) wherein are arranged parallel to one another a plurality of hollow fibers (3), whereof the porous wall (3a) of each defines an aperture (4), so that said means comprises a inner path and an outer path (B) corresponding to the volume available between the fibers and the disc separator for treating an initial sample with large volume comprising in diluted state a plurality of micro-organisms of different sizes. The invention is characterised in that during at least a concentration step, one single means of filtering is available, the atomic mass unit threshold of the porous wall (3a) of the fibers is determined so as to retain the smallest micro-organism of the initial sample, said means being used in frontal mode and discontinuously, thereby providing the output sample liquid, that is the concentrate.
摘要翻译：本发明涉及使用包括壳体（2）的过滤装置，其中彼此平行地布置有多个空心纤维（3），其中多孔壁（3a）限定孔（4），使得所述装置包括对应于纤维和盘分离器之间可用的体积的内部路径和外部路径（B），用于处理具有大体积的初始样品，其中稀释状态包含多种不同尺寸的微生物。本发明的特征在于，在至少一个浓缩步骤期间，可以使用一种单一的过滤方式，确定纤维的多孔壁（3a）的原子质量单位阈值，以便保留纤维的最小微生物初始样品，所述装置在正面模式下不连续地使用，从而提供输出样品液体，即浓缩液。

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