专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08679829B2 Archaeon expression system 失效
标题翻译：古生物表达系统
公开(公告)号：US08679829B2
公开(公告)日：2014-03-25
申请号：US10559583
申请日：2004-06-02
申请人： Christa Schleper , Melanie Jonuscheit , Jürgen Eck , Frank Niehaus , Sonja-Verena Albers , Sabrina Froels
发明人： Christa Schleper , Melanie Jonuscheit , Jürgen Eck , Frank Niehaus , Sonja-Verena Albers , Sabrina Froels
IPC分类号： C12N15/00 , C12N15/866 , C12N15/70 , C12N15/11
CPC分类号： C12N15/74
摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.
摘要翻译：本发明涉及一种磺叶病毒表达载体，其包含：（a）磺基梭菌复制起点; （b）编码与表达控制序列和包装信号有效连接的结构蛋白和SSV1，SSV2或pSSVx的位点特异性整合酶的基因; （c）一个或多个可选择的标记基因，其可操作地连接到子宫颈表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'端的磺基叶绿体启动子。此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中扩增和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制的大肠杆菌; 和（b）用于在大肠杆菌中选择的标记物。此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。最后，本申请还涉及产生感染性亚病毒颗粒的方法。

IPRDB

热门服务

关于我们

友情链接

联系方式