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1. 发明申请

US20120276578A1 Phasor Method to Fluorescence Lifetime Microscopy to Discriminate Metabolic State of Cells in Living Tissue 审中-公开
标题翻译：荧光终生显微镜的相量方法来鉴别生活组织中细胞的代谢状态
公开(公告)号：US20120276578A1
公开(公告)日：2012-11-01
申请号：US13283356
申请日：2011-10-27
申请人： Chiara Stringari , Enrico Gratton , Michelle Digman , Peter Donovan
发明人： Chiara Stringari , Enrico Gratton , Michelle Digman , Peter Donovan
IPC分类号： G01N21/64 , C12M1/34
CPC分类号： G01N21/6486 , G01N21/6408 , G01N21/6458
摘要： “A label-free imaging method to monitor stem cell metabolism discriminates different states of stem cell as they differentiate in a living tissues. We use intrinsic fluorescence biomarkers and the phasor approach to Fluorescence Lifetime Imaging Microscopy (FLIM). We identify and map intrinsic fluorophores such as collagen, retinol, retinoic acid, flavins, nicotinamide adenine dinucleotide (NADH) and porphyrin. We measure the phasor values of germ cells in C. Elegans germ line. Their metabolic fingerprint cluster according to their differentiation state, reflecting changes in FAD concentration and NADH binding during the differentiation pathway. The phasor approach to lifetime imaging provides a label-free, fit-free and sensitive method to identify different metabolic state of cells during differentiation, to sense small changes in the redox state of cells and may identify symmetric and asymmetric divisions and predict cell fate.”
摘要翻译：监测干细胞代谢的无标记成像方法会区分干细胞在活组织中分化的不同状态。我们使用内在荧光生物标志物和荧光寿命成像显微镜（FLIM）的相量方法。我们识别和映射内在的荧光团，如胶原，视黄醇，视黄酸，黄素，烟酰胺腺嘌呤二核苷酸（NADH）和卟啉。我们测量C. Elegans种系中生殖细胞的相量值。他们的代谢指纹簇根据其分化状态，反映分化途径中FAD浓度和NADH结合的变化。终生成像的相量方法提供了无标记，无配对和敏感的方法，以鉴别细胞在分化期间的不同代谢状态，以感测细胞氧化还原状态的小变化，并且可以鉴定对称和不对称分裂并预测细胞命运。

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