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1. 发明申请

US20130302867A1 METHOD FOR PRODUCING BIODEGRADABLE POLYMER AND BIOMASS FUEL CONVERTED FROM CARBON SOURCE BY RECOMBINANT MICROORGANISMS 审中-公开
标题翻译：通过重组微生物生产碳源可生物降解聚合物和生物质燃料的方法
公开(公告)号：US20130302867A1
公开(公告)日：2013-11-14
申请号：US13469945
申请日：2012-05-11
申请人： Chi-Wei Lan , Ho-Shing Wu , Feng-Shen Chiu
发明人： Chi-Wei Lan , Ho-Shing Wu , Feng-Shen Chiu
IPC分类号： C12P7/42
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12P7/06 , C12P7/08 , C12Y101/01021 , C12Y101/01036 , C12Y102/01003 , C12Y203/01 , C12Y203/01009 , Y02E50/17
摘要： Disclosed is a method for producing biodegradable polymer and biomass fuel converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w).
摘要翻译：本发明公开了一种通过使用重组微生物从碳源转化生物可降解聚合物和生物质燃料的方法，包括以下步骤：（A）提供用至少含有编码甘油的基因的质粒转化的重组微生物，并使用编码聚羟基链烷酸酯的基因合酶（B）在含有甘油的培养基中培养重组微生物; （C）诱导步骤（A）的基因的表达，从而获得聚羟基链烷酸酯和乙醇; 和（D）回收聚羟基链烷酸酯和乙醇; 其中所述重组微生物的甘油利用率大于90％（w / w），并且其中积聚有聚羟基链烷酸酯的生物质含量至少为30％（w / w）。

2. 发明申请

US20130122559A1 METHOD FOR SYNTHESIZING POLYHYDROXYALKANOATE USING A MICROORGANISM 审中-公开
标题翻译：使用微生物合成聚羟基烷基酯的方法
公开(公告)号：US20130122559A1
公开(公告)日：2013-05-16
申请号：US13296588
申请日：2011-11-15
申请人： Chi-Wei Lan , Cheng-Yung Chang
发明人： Chi-Wei Lan , Cheng-Yung Chang
IPC分类号： C12P7/62
CPC分类号： C12P7/625 , Y02E50/13
摘要： A method for synthesizing polyhydroxyalkanoate using a microorganism includes providing a Ralstonia eutropha strain; providing a fermentation tank and adding crude glycerol into the fermentation tank; adding a nitrogen source into the fermentation tank to adjust a carbon to nitrogen (C/N) ratio to 20:1 to 150:1; controlling temperature of the fermentation tank to a temperature ranging from 20 to 45° C.; adding 5 to 20% by volume of the Ralstonia eutropha strain into the fermentation tank using a mixing speed of from 100 to 500 rpm, an aeration quantity of 0.5-2 vvm and a concentration of dissolved oxygen of 30 to 80%; fermenting contents of the fermentation tank for 48 to 96 hours to allow the Ralstonia eutropha strain to proliferate and to allow polyhydroxyalkanoate to be synthesized from the crude glycerol within cells of the Ralstonia eutropha strain; and disrupting the cells of the Ralstonia eutropha strain and extracting polyhydroxyalkanoate.
摘要翻译：使用微生物合成聚羟基链烷酸酯的方法包括提供罗耳氏菌eutropha菌株; 提供发酵罐并将粗甘油加入到发酵罐中; 向发酵罐中加入氮源以将碳与氮（C / N）的比例调节为20：1至150：1; 将发酵罐的温度控制在20〜45℃的温度范围内。使用100〜500rpm的混合速度，通气量为0.5〜2vvm，溶解氧浓度为30〜80％，将5〜20体积％的Ralstonia eutropha菌株加入到发酵罐中。将发酵罐的发酵内容物发酵48〜96小时，以使雷氏菌属菌素菌株增殖，并且可以从拉尔斯通氏菌属细菌菌株的细胞内的粗甘油合成聚羟基链烷酸酯; 并破坏罗氏扁豆菌的细胞并提取聚羟基链烷酸酯。

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