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1. 发明申请

US20090234113A1 MICROBIAL CONSORTIA AND METHODS FOR THEIR USE 有权
标题翻译： MICROBIAL CONSORTIA及其使用方法
公开(公告)号：US20090234113A1
公开(公告)日：2009-09-17
申请号：US12366115
申请日：2009-02-05
申请人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
发明人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
IPC分类号： C13K13/00 , C12N1/20 , A62D3/02
CPC分类号： C12P39/00 , A62D3/02 , A62D2101/22 , C02F3/341 , C02F2101/36 , C12N1/20
摘要： Isolated microbial consortia capable of degrading chlorinated carbohydrates and a method to acclimatize microbes to degrade chlorinated carbohydrates under specific conditions of temperature and salt are described. Also described is a method for using microbial consortia to degrade chlorinated carbohydrates in a waste stream.
摘要翻译：描述了能够降解氯化碳水化合物的分离的微生物联合体和在温度和盐的特定条件下使微生物驯化以降解氯化碳水化合物的方法。还描述了使用微生物菌落来降解废物流中的氯化碳水化合物的方法。

2. 发明授权

US08557976B2 Microbial consortia and methods for their use 有权
标题翻译：微生物联盟及其使用方法
公开(公告)号：US08557976B2
公开(公告)日：2013-10-15
申请号：US12366115
申请日：2009-02-05
申请人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
发明人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
IPC分类号： C13K13/00
CPC分类号： C12P39/00 , A62D3/02 , A62D2101/22 , C02F3/341 , C02F2101/36 , C12N1/20
摘要： Isolated microbial consortia capable of degrading chlorinated carbohydrates and a method to acclimatize microbes to degrade chlorinated carbohydrates under specific conditions of temperature and salt are described. Also described is a method for using microbial consortia to degrade chlorinated carbohydrates in a waste stream.
摘要翻译：描述了能够降解氯化碳水化合物的分离的微生物联合体和在温度和盐的特定条件下使微生物驯化以降解氯化碳水化合物的方法。还描述了使用微生物菌落来降解废物流中的氯化碳水化合物的方法。

3. 发明申请

US20060251761A1 Grain wet milling process for producing dextrose 审中-公开
标题翻译：用于生产葡萄糖的谷物湿磨工艺
公开(公告)号：US20060251761A1
公开(公告)日：2006-11-09
申请号：US11121170
申请日：2005-05-03
申请人： Robert Jansen , John Kerr , Edward Farley , Gordon Walker , Sebastien Camborieux
发明人： Robert Jansen , John Kerr , Edward Farley , Gordon Walker , Sebastien Camborieux
IPC分类号： A23L1/10
CPC分类号： C12Y302/00 , A23J1/125 , A23J3/18 , A23K10/30 , A23K20/179 , A23K50/80 , A23L7/104 , A23L29/35 , C13B20/142 , C13K1/08 , Y02A40/818
摘要： Whole grain, such as wheat, barley, rye, and/or rice, can be processed by (a) steeping the grain or at least partially dehulled grain in an aqueous liquid to produce softened grain, (b) milling the softened grain to produce milled grain, (c) liquefying the milled grain by contacting it with amylase and heating it to a temperature of at least about 50° C., producing a liquefied material, (d) at least partially saccharifying the liquefied material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a first saccharified material, and (e) separating fiber and germ from the first saccharified material, producing a screened material that is substantially free of fiber and wheat germ. The process also includes the steps of (f) further saccharifying the screened material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a second saccharified material, (g) membrane filtering the second saccharified material, producing a permeate that comprises primarily dextrose and other soluble components and a retentate that comprises insoluble protein, and (h) purifying the permeate by chromatographic separation, producing a purified dextrose stream. The chromatographic separation can also produce a raffinate, and the process can further include the steps of (i) combining the retentate from the membrane filtration and the raffinate from the chromatographic separation to form a fermentation medium, (j) fermenting the fermentation medium aerobically with a microorganism, (k) separating a protein product that comprises insoluble protein and microorganism from the medium, and (l) drying the protein product.
摘要翻译：可以通过（a）在水性液体中浸渍颗粒或至少部分去壳的颗粒以产生软化的颗粒，（b）研磨软化的颗粒以产生（例如，麦粒，大麦，黑麦和/或米）（c）通过使其与淀粉酶接触并将其加热到至少约50℃的温度来液化所研磨的颗粒，产生液化材料，（d）通过使液化物质与淀粉葡糖苷酶接触而至少部分地糖化在至少约50℃的温度下，产生第一糖化材料，和（e）从第一糖化物质分离纤维和胚芽，产生基本上不含纤维和小麦胚芽的筛选材料。该方法还包括以下步骤：（f）通过使筛选的材料与淀粉葡糖苷酶在至少约50℃的温度下接触进一步糖化，产生第二糖化物质，（g）膜过滤第二糖化物质，产生主要包含葡萄糖和其他可溶性组分的渗透物和包含不溶性蛋白质的渗余物，和（h）通过色谱分离纯化渗透物，产生纯化的葡萄糖流。色谱分离还可以产生萃余液，并且该方法还可以包括以下步骤：（i）将来自膜过滤的残留物和来自色谱分离的萃余液组合以形成发酵培养基，（j）有氧地发酵发酵培养基，微生物，（k）从培养基中分离含有不溶性蛋白质和微生物的蛋白质产物，和（l）干燥蛋白质产物。

4. 发明申请

US20060251762A1 Grain wet milling process for producing ethanol 审中-公开
标题翻译：谷物湿磨生产乙醇的方法
公开(公告)号：US20060251762A1
公开(公告)日：2006-11-09
申请号：US11121295
申请日：2005-05-03
申请人： Robert Jansen , John Kerr , Edward Farley , Gordon Walker , Sebastien Camborieux
发明人： Robert Jansen , John Kerr , Edward Farley , Gordon Walker , Sebastien Camborieux
IPC分类号： A23L1/10
CPC分类号： C12P7/06 , Y02E50/16 , Y02E50/17
摘要： Whole grain, such as wheat, barley, rye, and/or rice, can be processed by (a) steeping the grain in an aqueous liquid to produce softened grain, (b) milling the softened grain to produce milled grain, (c) liquefying the milled grain by contacting it with amylase and heating it to a temperature of at least about 50° C., producing a liquefied material, (d) at least partially saccharifying the liquefied material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a first saccharified material, and (e) separating fiber and germ from the first saccharified material, producing a screened material that is substantially free of fiber and germ. The process also includes the steps of (f) further saccharifying and fermenting the screened material with a microorganism that produces ethanol, thereby producing a broth that comprises ethanol, soluble protein, and insoluble protein, and (g) separating ethanol from the broth. A protein-rich product can be recovered from the broth that comprises both gluten from the grain and microorganism from the fermenting step.
摘要翻译：（a）在水溶液中浸渍谷物以产生软化谷粒，（b）研磨软化谷物以产生碾磨的谷物，（c）可以加工全谷物如小麦，大麦，黑麦和/或米，（c）通过使其与淀粉酶接触并将其加热到至少约50℃的温度来液化碾磨的颗粒，产生液化材料，（d）至少部分地使液化物料与淀粉葡糖苷酶接触至少部分糖化，约50℃，产生第一糖化物质，和（e）从第一糖化物质分离纤维和胚芽，产生基本上不含纤维和细菌的筛选材料。该方法还包括以下步骤：（f）用生产乙醇的微生物进一步对筛选的材料进行糖化和发酵，从而产生包含乙醇，可溶性蛋白质和不溶性蛋白质的肉汤，和（g）从肉汤中分离乙醇。富含蛋白质的产物可以从含有谷物的谷蛋白和发酵步骤的微生物的肉汤中回收。

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