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1. 发明授权

US09302194B2 Detachable hula hoop having waterproof reservoirs 有权
标题翻译：可分离的呼啦圈具有防水水库
公开(公告)号：US09302194B2
公开(公告)日：2016-04-05
申请号：US14738826
申请日：2015-06-12
申请人： Cheng-Hsiung Hsu , Su-Chen Li
发明人： Cheng-Hsiung Hsu , Su-Chen Li
IPC分类号： A63H33/02 , A63H33/00 , A63B19/00
CPC分类号： A63H33/00 , A63B19/00 , A63B2210/50
摘要： A detachable hula hoop having waterproof reservoirs is provided with hollow arc sections each including a neck; a first connection end at a first end and including an opening, a channel, and a first recess on a surface; a plug including a cylindrical member and a head; a second connection end at a second end and including a closed member and a second recess on a surface; and a reservoir. The first recess is complimentarily disposed in the second recess to fasten the first and second connection ends together. The cylindrical member is disposed in the neck, the head urges against an annular shoulder to block the neck, and the head is urged against by the closed member.
摘要翻译：具有防水容器的可分离的呼啦圈设置有各自包括颈部的中空弧形部分; 在第一端处的第一连接端，并且包括开口，通道和表面上的第一凹部; 包括圆柱形构件和头部的塞子; 在第二端处的第二连接端，并且包括封闭构件和表面上的第二凹部; 和一个水库。第一凹部被补充地设置在第二凹部中，以将第一和第二连接端紧固在一起。圆柱形构件设置在颈部中，头部抵靠环形肩部阻止颈部，并且头部被封闭构件推靠。

2. 发明授权

US5047337A Ceramide-glycanase 失效
标题翻译：神经酰胺 - 聚糖酶
公开(公告)号：US5047337A
公开(公告)日：1991-09-10
申请号：US113024
申请日：1987-10-23
申请人： Su-Chen Li , Yu-Teh Li
发明人： Su-Chen Li , Yu-Teh Li
IPC分类号： C12N9/24 , C12P19/04
CPC分类号： C12Y302/01123 , C12N9/2402 , C12P19/04
摘要： Ceramide-glycanase which cleaves the entire oligosaccharides intact from glycosphingolipids and alkylglycolipids. The enzyme does not hydrolyze monosaccharides from these glycoconjugates. The enzyme is obtained from the muscle tissue of annelids such as earthworms and leeches by homogenization in water, ammonium sulfate fractionation, gel permeation chromatography, and hydrophobic interaction chromatography, and is essentially free from exo-glycosidase activity. Also disclosed is a method for obtaining oligosaccharides by hydrolysis of glycolipids, alkylglycolipids, and glycosphingolipids by the ceramide-glycanase.
摘要翻译：从糖鞘脂和烷基糖脂中切割整个寡糖的神经酰胺 - 聚糖酶。该酶不会从这些糖缀合物中水解单糖。该酶通过在水中均质化，硫酸铵分级分离，凝胶渗透色谱法和疏水相互作用色谱法从天ids素如蚯蚓和水蛭的肌肉组织获得，并且基本上不含外切糖苷酶活性。还公开了通过神经酰胺 - 聚糖酶水解糖脂，烷基糖脂和糖鞘脂来获得寡糖的方法。

3. 发明授权

US5449615A KDN-cleaving sialidase isolated from hepatopancreas of mollusks 失效
标题翻译：从软体动物肝胰腺分离的KDN切割唾液酸酶
公开(公告)号：US5449615A
公开(公告)日：1995-09-12
申请号：US311957
申请日：1994-09-26
申请人： Yuh-Teh Li , Su-Chen Li
发明人： Yuh-Teh Li , Su-Chen Li
IPC分类号： C12N9/24 , C12N9/36
CPC分类号： C12Y302/01129 , C12N9/2402 , C12Y302/01018
摘要： A sialidase capable of cleaving a glycoconjugate to yield 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) is isolated from hepatopancreas of mollusks. Preferably, the sialidase is isolated by processing crude oyster hepatopancreas to form a crude extract of the sialidase enzyme. The crude enzyme is further concentrated and refined utilizing sequential column chromatography fractogel EMD DEAE-650 (M), sephacryl S-200, and fractogel EMD SP 650 (M) chromatography, respectively. Using this procedure, the sialidase is purified over 155 fold with a 17% recovery. Ferric ion exerts a three fold stimulation in the activity of the sialidase, at a concentration of 3 mM. The sialidase cleaves 4-methylumbelliferyl-KDN, KDN.alpha.2.fwdarw.3Gal.beta.1.fwdarw.4GlcCer, KDN.alpha.2.fwdarw.6Gal.beta.1.fwdarw.4GlcCer, or KDN.alpha.2.fwdarw.6GalNAc-o1 to yield KDN.
摘要翻译：能够切割糖缀合物以产生3-脱氧-D-甘油-D-半乳-2-异壬酸（KDN）的唾液酸酶从软体动物的肝胰腺中分离。优选地，通过加工粗牡蛎肝胰腺来分离唾液酸酶以形成唾液酸酶的粗提取物。使用顺序柱色谱分离凝胶EMD DEAE-650（M），sephacryl S-200和分子凝胶EMD SP 650（M）色谱法分离粗酶。使用该方法，将唾液酸酶以17％的回收率纯化155倍。铁离子以3mM的浓度对唾液酸酶的活性施加三倍的刺激。唾液酸酶切割4-甲基伞形酮基KDN，KDNα2->3Galβ1-> 4GlcCer，KDNα2->6Galβ1-> 4GlcCer或KDNα2-> 6GalNAc-o1以产生KDN。

4. 发明授权

US5312747A Isolation, purification and utilization of sialidase-L in the synthesis of 2,7-anhydro-N-acetylneuraminic acid and for the selective cleavage of sialyl.alpha.2-3 D-galactose linkage 失效
标题翻译：唾液酸酶-L在2,7-脱水N-乙酰神经氨酸的合成中的分离，纯化和利用以及唾液酸α2-3 D-半乳糖键的选择性切割
公开(公告)号：US5312747A
公开(公告)日：1994-05-17
申请号：US015413
申请日：1993-02-08
申请人： Yu-Teh Li , Su-Chen Li
发明人： Yu-Teh Li , Su-Chen Li
IPC分类号： C07K14/705 , C12N9/24 , C12P19/26 , C12N9/20 , C12N9/26
CPC分类号： C12Y402/02015 , C07K14/70564 , C12N9/88 , C12P19/26 , Y10S435/815
摘要： The instant invention relates to the isolation, purification and utilization of sialidase-L, a novel enzyme capable of producing 2,7-anhydro N-acetylneuraminic acid by the selective cleavage of sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic bonds. The instant invention also relates to a method for the preparation of 2,7-anhydro-N-acetylneuraminic acid using sialidase-L. The instant invention also relates to methods for selectively cleaving sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic linkages and providing a means to selectively destroy the selectin ligands for the treatment of selectin-mediated inflammation. The instant invention also relates to methods for using 2,7-anhydro-N-acetylneuraminic acid and sialidase-L for the selective sialylation of acceptor glycosides to form sialic acid-.alpha.2.fwdarw.3D-galactose glycosidic linkages.
摘要翻译：本发明涉及唾液酸酶-L的分离，纯化和利用，唾液酸酶-L是一种能够通过唾液酸α2-> 3D-半乳糖糖苷键的选择性裂解产生2,7-脱水N-乙酰神经氨酸的新型酶。本发明还涉及使用唾液酸酶-L制备2,7-脱水-N-乙酰神经氨酸的方法。本发明还涉及用于选择性切割唾液酸-α2-> 3D-半乳糖糖苷键的方法，并提供选择性地破坏选择素配体以治疗选择素介导的炎症的方法。本发明还涉及使用2,7-脱水-N-乙酰神经氨酸和唾液酸酶-L用于选择性唾液酸化受体糖苷以形成唾液酸-α2-> 3D-半乳糖糖苷键的方法。

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