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    • 3. 发明授权
    • Methods for detecting and quantifying occult blood
    • 隐匿性血液检测和定量方法
    • US4277250A
    • 1981-07-07
    • US101722
    • 1979-12-10
    • Joseph L. MelnickCraig Wallis
    • Joseph L. MelnickCraig Wallis
    • G01N33/50C12Q1/28G01N33/72
    • G01N33/725C12Q1/28C12Q2326/00Y10T436/25Y10T436/25375
    • A method for detecting occult blood in aqueous samples of human specimens, particularly aqueous suspensions of fecal matter is disclosed. The method comprises acidifying an aqueous test sample, which in the case of fecal matter has been treated with lysozyme to clear the suspension; separating the liquid and solid portions of the test sample and passing the acidified liquid portion through an electro-negatively charged membrane; thereafter removing residual test liquids from the membrane; treating the membrane with a peroxidase indicator and observing the development of color on the treated membrane surface. A method for quantifying occult blood is also disclosed which comprises subjecting an aqueous sample to the disclosed detection method and comparing the color developed with a standardized scale reflecting colors developed by treating samples containing known incremental quantities of hemoglobin in accordance with the detection method of the invention.
    • 公开了一种用于检测人体样品的水样品中的潜血的方法,特别是粪便物的水悬浮液。 该方法包括酸化水性测试样品,在用溶菌酶处理粪便物质的情况下,以清除悬浮液; 分离测试样品的液体和固体部分,并使酸化的液体部分通过电荷带负电的膜; 然后从膜中除去残留的测试液体; 用过氧化物酶指示剂处理膜并观察处理的膜表面上的颜色的发展。 还公开了一种量化隐血的方法,其包括使水样品进行所公开的检测方法,并根据本发明的检测方法比较显影的颜色与反映根据本发明的检测方法处理含有已知增加量的血红蛋白的样品开发的颜色的标准化比例 。
    • 4. 发明授权
    • Sterilization of holding tanks and toilet bowls by quaternary compounds
    • 通过四级化合物灭菌储罐和厕所碗
    • US3941696A
    • 1976-03-02
    • US561447
    • 1975-03-24
    • Joseph L. MelnickCraig Wallis
    • Joseph L. MelnickCraig Wallis
    • A01N33/12A01N43/40A61L9/01C02F1/50C02B3/06
    • C02F1/50A01N33/12A01N43/40A61L9/01
    • Wastewaters heavily laden with organic compounds and containing viruses and other microbiological flora are sterilized and maintained sterile for prolonged periods of time by the addition of a base, such as lime, and a quaternary compound, preferably cetyl pyridinium chloride, in amounts to maintain the pH of the wastewaters at about 9-11, or higher, and with sufficient quaternary compound for suspended solids present. This prevents the formation of methane gases, which are tentative hazards in that they are inflammable, asphyxiant and narcotic. Similarly, toilets are rendered sterile by the addition of the base and quaternary compound so that, upon flushing, aerosols generated will not contain active infectious agents. All bacteria and fungi are inactivated under these basic conditions by the addition of quaternary compounds. Viruses are also inactivated under these basic conditions but only with quaternary compounds which contain the pyridinium moiety. The disclosure includes products for and methods of sterilizing wastewaters. A number of examples are given.
    • 含有有机化合物和含有病毒的其他微生物菌群的废水通过加入碱(例如石灰)和季铵化合物(优选十六烷基氯化吡啶)灭菌并长时间维持无菌,其用量为维持pH 的废水在约9-11或更高,并且具有足够的季铵化合物用于悬浮固体存在。 这样可以防止甲烷气体的形成,这是因为易燃,窒息和麻醉而引起的暂时危害。 类似地,通过添加碱和季铵化合物使厕所无菌,使得在冲洗时,产生的气溶胶将不含有活性感染剂。 所有细菌和真菌在这些基本条件下通过添加季铵化合物而失活。 在这些基本条件下,病毒也被灭活,但只有含有吡啶鎓部分的季铵化合物。 本公开包括废物的消毒和消毒方法。 给出了一些例子。
    • 5. 发明授权
    • Staining and analysis of bacteria
    • 染色和分析细菌
    • US4225669A
    • 1980-09-30
    • US33900
    • 1979-04-27
    • Joseph L. MelnickCraig Wallis
    • Joseph L. MelnickCraig Wallis
    • C12Q1/04C12Q1/06C12Q1/18
    • C12Q1/04
    • A bacterial staining composition and methods of analysis of both gram-negative and gram-positive bacteria are disclosed. The composition comprises a chelating agent and a basic dye, both of which are operative at a pH above about 7.0. Bacterial staining may be effected by contacting either concentrated or fluidly suspended bacteria with the composition at a neutral or basic pH. Bacteria stained with the composition and concentrated by filtration, centrifugation or the like are readily visible and their presence in a specimen may, thus, be rapidly detected. The gradations of color of the stained, concentrated bacteria correspond to the number of bacteria and semi-quantitative analysis of the bacteria may be effected by comparison with a standard. Differentiation of gram-negative and gram-positive bacteria may be accomplished by treating the stained bacteria with an organic acid wash having a pH of about 2.5 to 2.6. Such a wash completely decolorizes only stained gram-positive bacteria. Finally, a method for determining bacterial susceptibility to antimicrobial agents is provided which comprises incubating bacteria with an antimicrobial agent, staining the bacteria and thereafter comparing the color gradation of the stained, concentrated bacteria with a control or standard.
    • 公开了革兰氏阴性细菌和革兰氏阳性菌的细菌染色组合物和分析方法。 该组合物包含螯合剂和碱性染料,它们都在高于约7.0的pH下操作。 细菌染色可以通过在中性或碱性pH下使浓缩的或流体悬浮的细菌与组合物接触来实现。 用组合物染色的细菌并通过过滤浓缩,离心等容易可见,并且因此可以快速检测其在样品中的存在。 染色的浓缩细菌的颜色等级与细菌数量相对应,细菌的半定量分析可以通过与标准进行比较来实现。 革兰氏阴性和革兰氏阳性菌的分化可以通过用pH为约2.5至2.6的有机酸洗涤处理染色的细菌来实现。 这样的洗涤仅使染色的革兰氏阳性细菌脱色。 最后,提供了一种用于测定抗微生物剂的细菌易感性的方法,其包括用细菌培养细菌与抗微生物剂,染色细菌,然后比较染色的浓缩细菌的颜色等级与对照品或标准品。
    • 6. 发明授权
    • Sterilization of holding tanks and toilet bowls by quaternary compounds
    • 通过四级化合物灭菌储罐和厕所碗
    • US4043911A
    • 1977-08-23
    • US662072
    • 1976-02-27
    • Joseph L. MelnickCraig Wallis
    • Joseph L. MelnickCraig Wallis
    • A01N33/12A01N43/40A61L9/01C02F1/50C02B3/06
    • C02F1/50A01N33/12A01N43/40A61L9/01
    • Wastewaters heavily laden with organic compounds and containing viruses and other microbiological flora are sterilized and maintained sterile for prolonged periods of time by the addition of a base, such as lime, and a quaternary compound, preferably cetyl pyridinium chloride, in amounts to maintain the pH of the wastewaters at about 9-11, or higher, and with sufficient quaternary compound for suspended solids present. This prevents the formation of methane gases, which are tentative hazards in that they are inflammable, asphyxiant and narcotic. Similarly, toilets are rendered sterile by the addition of the base and quaternary compound so that, upon flushing, aerosols generated will not contain active infectious agents. All bacteria and fungi are inactivated under these basic conditions by the addition of quaternary compounds. Viruses are also inactivated under these basic conditions but only with quaternary compounds which contain the pyridinium moiety. The disclosure includes products for and methods of sterilizing wastewaters. A number of examples are given.
    • 含有有机化合物和含有病毒的其他微生物菌群的废水通过加入碱(例如石灰)和季铵化合物(优选十六烷基氯化吡啶)灭菌并长时间维持无菌,其用量为维持pH 的废水在约9-11或更高,并且具有足够的季铵化合物用于悬浮固体存在。 这样可以防止甲烷气体的形成,这是因为易燃,窒息和麻醉而引起的暂时危害。 类似地,通过添加碱和季铵化合物使厕所无菌,使得在冲洗时,产生的气溶胶将不含有活性感染剂。 所有细菌和真菌在这些基本条件下通过添加季铵化合物而失活。 在这些基本条件下,病毒也被灭活,但只有含有吡啶鎓部分的季铵化合物。 本公开包括废物的消毒和消毒方法。 给出了一些例子。
    • 10. 发明授权
    • Determination of bacterial growth activity and antibiotic sensitivity by
catalase measurement
    • 通过过氧化氢酶测定法测定细菌生长活性和抗生素敏感性
    • US4311794A
    • 1982-01-19
    • US160682
    • 1980-06-18
    • Joseph L. MelnickCraig Wallis
    • Joseph L. MelnickCraig Wallis
    • C12M1/34C12Q1/18C12Q1/30
    • C12Q1/18C12M23/04C12M41/40C12M41/46C12Q1/30
    • A catalase method and apparatus are disclosed for direct measurement of bacterial growth activity in the presence and absence of antibiotics, and for the determination of antibiotic sensitivity directly from clinical specimens within one and one-half to four or five hours if the agent is a catalase producer. If not, antibiotic sensitivity is measured by light obscuration. The total bacterial counts of normal flora and artifacts in the growth medium do not affect the tests. Preferably, a transducer or other pressure-measuring technique determines the positive pressure produced by the catalytic decomposition of peroxide. Aerosolization of pathogenic organisms is avoided by combining hydrogen peroxide with a quaternary compound which kills the bacteria and at the same time frees additional catalase to yield total catalase titers which are very reproducible and accurate. The method and apparatus easily detect Staphylococcus, Pseudomonas, Proteus, E. coli, and other catalase-producing bacteria. The method and apparatus can be used to determine bacterial growth activity in many liquids for example, cutting oils, water used for drinking, pharmaceuticals, swimming pools, cooling towers and for other purposes as well as with body fluids both qualitatively and quantitatively.
    • 公开了一种过氧化氢酶方法和装置,用于在存在和不存在抗生素的情况下直接测量细菌生长活性,并且如果该试剂是过氧化氢酶,则直接从临床标本中测定抗生素敏感性在一个半至四个或四个或五个小时内 生产者。 如果没有,抗生素敏感性是通过光遮蔽来测量的。 生长培养基中正常菌群和人工制品的总细菌数不影响试验。 优选地,换能器或其它压力测量技术确定由过氧化物的催化分解产生的正压力。 通过将过氧化氢与杀死细菌的季铵化合物结合,同时释放额外的过氧化氢酶以产生非常可重现和准确的总过氧化氢酶滴度来避免病原生物体的气溶胶化。 该方法和装置易于检测葡萄球菌,假单胞菌,变形杆菌,大肠杆菌等产生过氧化氢酶的细菌。 该方法和装置可用于确定许多液体中的细菌生长活性,例如切割油,用于饮用的饮用水,药物,游泳池,冷却塔以及用于其它目的以及定量和定量的体液。