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    • 6. 发明授权
    • Electrophoresis gel and gel-forming apparatus
    • 电泳凝胶和凝胶形成装置
    • US07060172B1
    • 2006-06-13
    • US09647821
    • 1999-04-09
    • David SolomonGrace Chan
    • David SolomonGrace Chan
    • C07K1/26
    • G01N27/44704
    • The apparatus includes a container (20) having a base (22) and sides (32) adapted to receive a plurality of plastic gel cassettes. An inlet port (12) is positioned in the base of the container and in fluid communication with the chamber and a baffle (11) is positioned over the inlet port, such that, in use, when gel forming fluid passes through the inlet port into the chamber, the baffle substantially reduces fluid turbulence and vertical fluid movement in the vicinity of the inlet port during flow of the fluid into the chamber. Pretreatment of the plastic cassette to remove polymerisation inhibitors prior to filling the same with fluid is by exhaustive vacuum treatment, optionally with nitrogen gas purging. This can be achieved conveniently using a vacuum chamber in which one or more plastic cassettes are placed. Optionally the vacuum chamber may be the container in which the cassettes are filled with fluid. No barrier films or chemical scavengers are required.
    • 该装置包括具有底座(22)和适于容纳多个塑料胶带的侧面(32)的容器(20)。 入口端口(12)定位在容器的基部中并与腔室流体连通,并且挡板(11)位于入口端口上方,使得在使用中,当凝胶形成流体通过入口端口时 在流体进入腔室期间,挡板基本上减少流体湍流和在入口附近的垂直流体运动。 在用液体填充之前,预先处理塑料盒以除去聚合抑制剂是通过彻底的真空处理,任选地用氮气吹扫。 这可以使用其中放置一个或多个塑料盒的真空室方便地实现。 可选地,真空室可以是盒中充满流体的容器。 不需要阻隔膜或化学清除剂。