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    • 6. 发明申请
    • Polypeptide
    • 多肽
    • US20060018997A1
    • 2006-01-26
    • US10886527
    • 2004-07-07
    • Karsten KraghBo Sorensen
    • Karsten KraghBo Sorensen
    • A21D8/02C12N1/21
    • C12N9/2417A21D8/042
    • We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 161 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 223, preferably 121F, 121Y, 121W, 223E and 223K, more preferably G121F, G121Y, G121W, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.
    • 我们描述了一种包含PS4变体多肽的食品添加剂,其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽,其中PS4变体多肽包含参考位置编号在161位置的氨基酸取代 的SEQ ID NO:1所示的假单胞菌糖酵母外切酶序列.PS4变体多肽可以在选自121和223,优选121F,121Y,121W,223E和223K的位置进一步包含一个或多个另外的突变 ,更优选G121F,G121Y,G121W,G223E和/或G223K,或由134,141,157,223,307,334,33和34,优选G134R,A141P,I157L,G223A,H307L,S334P, N33Y和D34N。 在优选的实施方案中,PS4变体多肽还包含位置87,优选G87S,178位突变,优选L178F和/或179位突变,优选A179T的突变。
    • 8. 发明申请
    • Polypeptide
    • 多肽
    • US20060008890A1
    • 2006-01-12
    • US10886505
    • 2004-07-07
    • Karsten KraghBo Sorensen
    • Karsten KraghBo Sorensen
    • C12N9/26C07H21/04C12P21/06C12N1/21
    • A21D8/042A23L33/18
    • We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 121 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 161 and 223, preferably 161A, 223E and 223K, more preferably S161A, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.
    • 我们描述了一种包含PS4变体多肽的食品添加剂,其中PS4变体多肽可衍生自具有非产麦芽糖外切酶活性的亲本多肽,其中PS4变体多肽包含参考位置编号在121位的氨基酸取代 的SEQ ID NO:1所示的假单胞菌糖酵母外切酶序列.PS4变体多肽可以在选自161和223,优选161A,223E和223K,更优选S161A的位置进一步包含一个或多个另外的突变 ,G223E和/或G223K,或由134,141,157,223,307,334,33和34,优选G134R,A141P,I157L,G223A,H307L,S334P,N33Y和D34N组成的组。 在优选的实施方案中,PS4变体多肽还包含位置87,优选G87S,178位突变,优选L178F和/或179位突变,优选A179T的突变。
    • 9. 发明申请
    • Polypeptide
    • 多肽
    • US20060073583A1
    • 2006-04-06
    • US10947612
    • 2004-09-22
    • Karsten KraghBo Sorensen
    • Karsten KraghBo Sorensen
    • A21D8/02C12N9/26C12P21/06C12N15/74C12N1/21
    • C12N9/2411A21D2/267A21D8/042C12N9/2414C12N9/2417
    • We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 146, 157, 158, 198, 229, 303, 306, 309, 316 and 353, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide comprises an amino acid mutation selected from the group consisting of: 146G, 146M, 157M, 158T, 158A, 158S, 198W, 198F, 229P, 303E, 303D, 306T, 306G, 309P, 316S, 316P, 316K, 316Q and 353T, preferably 146G, 157M, 158T, 198W, 229P, 303E, 303D, 306T, 306G, 309P, 316S, 316P or 353T. in highly preferred embodiments, the PS4 variant polypeptide has a sequence selected from SEQ ID NO: 15 to 28.
    • 我们描述了一种包含PS4变体多肽的食品添加剂,其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽,其中PS4变体多肽包含一个或多个选自下组的位置的氨基酸突变 包括:146,157,158,198,29,303,306,309,316和353,其参考如SEQ ID NO:1所示的假单胞菌属的嗜酸性粒细胞淀粉酶序列的位置编号。优选地,PS4变体多肽包含 选自146G,146M,157M,158T,158A,158S,198W,198F,229P,303E,303D,306T,306G,309P,316S,316P,316K,316Q和353T的氨基酸突变,优选 146G,157M,158T,198W,229P,303E,303D,306T,306G,309P,316S,316P或353T。 在非常优选的实施方案中,PS4变体多肽具有选自SEQ ID NO:15至28的序列。
    • 10. 发明申请
    • Polypeptide
    • 多肽
    • US20060008888A1
    • 2006-01-12
    • US10886504
    • 2004-07-07
    • Karsten KraghBo Sorensen
    • Karsten KraghBo Sorensen
    • C12N9/00C07H21/04C12P21/06
    • A21D8/042A23L33/18C12N9/2417C12P19/14
    • We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 223 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 161, preferably 121F, 121Y, 121W and 161A, more preferably G121F, G121Y, G121W and/or S161A, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.
    • 我们描述了包含PS4变体多肽的食品添加剂,其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽,其中PS4变体多肽包含参考位置编号在223位置的氨基酸取代 如SEQ ID NO:1所示的假单胞菌糖酵母外切酶序列.PS4变体多肽还可以在选自121和161,优选121F,121Y,121W和161A的位置进一步包含一个或多个另外的突变,更多 优选G121F,G121Y,G121W和/或S161A,或由134,141,157,223,307,334,33和34,优选G134R,A141P,I157L,G223A,H307L,S334P,N33Y和D34N组成的组。 在优选的实施方案中,PS4变体多肽还包含位置87,优选G87S,178位突变,优选L178F和/或179位突变,优选A179T的突变。