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1. 发明授权

US5200337A Type II restriction endonuclease, APO I, obtainable from arthrobacter protophormiae and a process for producing the same 失效
标题翻译：类型II限制性内切酶，APO I，可从阿尔沙门氏菌原生质体获得及其生产方法
公开(公告)号：US5200337A
公开(公告)日：1993-04-06
申请号：US782515
申请日：1991-10-25
申请人： Carol Polisson , Derek Robinson , Keith Lunnen
发明人： Carol Polisson , Derek Robinson , Keith Lunnen
IPC分类号： C12N9/12 , C12N9/10 , C12N9/16 , C12N9/22 , C12N15/09 , C12N15/55 , C12R1/06
CPC分类号： C12N9/22 , C12N9/1007
摘要： The present invention provides a novel type II restriction endonuclease obtainable from Arthrobacter protophormiae. The endonuclease known as Apo I, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining Apo I from Arthrobacter protophormiae.
摘要翻译：本发明提供了可从节杆菌（Arthrobacter protophormiae）获得的新型II型限制性内切核酸酶。称为Apo I的内切核酸酶识别以下核苷酸序列，并具有由箭头指示的切割点：还描述了从原生节杆菌获得Apo I的方法。

2. 发明授权

US5098839A Type II restriction endonuclease obtainable from Pseudomonas alcaligenes and a process for producing the same 失效
标题翻译：可从PSEUDOMONAS ALCALIGENES获得的II型限制性内切酶及其生产方法
公开(公告)号：US5098839A
公开(公告)日：1992-03-24
申请号：US521540
申请日：1990-05-10
申请人： Carol Polisson
发明人： Carol Polisson
IPC分类号： C12N9/16 , C12N9/22 , C12R1/38
CPC分类号： C12N9/22
摘要： The present invention provides a novel Type II restriction endonuclease obtainable from Pseudomonas alcaligenes. The endonuclease known as PacI, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining PacI from P. alcaligenes.

3. 发明授权

US5147794A Type II restriction endonuclease obtainable from Kluyvera ascorbata and a process for producing the same 失效
标题翻译：可从Kluyvera ascorbata获得的II型限制性内切核酸酶及其制备方法
公开(公告)号：US5147794A
公开(公告)日：1992-09-15
申请号：US557394
申请日：1990-07-23
申请人： Carol Polisson , Janet M. Barsomian
发明人： Carol Polisson , Janet M. Barsomian
IPC分类号： C12N9/10 , C12N9/22
CPC分类号： C12N9/1007 , C12N9/22
摘要： The present invention provides a novel restriction endonuclease obtainable from the bacterium Kluyvera ascorbata, hereinafter referred to as "KasI", which endonuclease:(1) recognizes the base sequence in a double-stranded DNA molecule as shown below,5'--GGCGCC--3'3'--CCGCGG--5' (wherein C and G represent cytosine and guanine, respectively),(2) cleaves said sequence in th4e phosphodiester bonds between G and G as indicated with the vertical arrows; and(3) cleaves double-stranded pUC, M13mp18, and lambda DNA in one position, PhiX172, and T7 DNA in two positions, and Adeno2 DNA at 20 positions, while not cleaving SV40 DNA.The present invention further provides a recombinant DNA encoding the KasI restriction endonuclease and modification methylase obtainable from K. ascorbata, and methods for the production of the recombinant DNA encoding those enzymes. Methods for producing the KasI restriction endonuclease and modification methylase in substantially pure form are also provided.
摘要翻译：本发明提供了可从克拉维酵母（Kluyvera ascorbata）获得的新型限制性内切核酸酶（以下称为“KasI”），该核酸内切酶：（1）识别如下所示的双链DNA分子中的碱基序列，5'-GGCGCC-3 '3'-CCGCGG-5'（其中C和G分别代表胞嘧啶和鸟嘌呤），（2）如垂直箭头所示，切割G和G之间的磷酸二酯键中的所述序列; 和（3）在两个位置切割一个位置的双链pUC，M13mp18和λDNA，两个位置的PhiX172和T7DNA，而在20个位置切割Adeno2 DNA，而不切割SV40DNA。本发明还提供了编码KasI限制性内切核酸酶的重组DNA和可从K. ascorbata获得的修饰甲基化酶，以及编码这些酶的重组DNA的制备方法。还提供了以基本上纯的形式生产KasI限制性内切核酸酶和修饰甲基化酶的方法。

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