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1. 发明申请

US20180327774A1 EPSPS MUTANTS 审中-公开
公开(公告)号：US20180327774A1
公开(公告)日：2018-11-15
申请号：US16040278
申请日：2018-07-19
申请人： CIBUS US LLC , INCIMA US LLC
发明人： Greg F.W. Gocal , Mark E. Knuth , Peter R. Beetham
IPC分类号： C12N15/82 , C12N9/10
CPC分类号： C12N15/8275 , C12N9/1092 , C12N15/8213
摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. Additionally the present invention relates to mutant E. coli cells that contain mutated EPSPS genes.

2. 发明申请

US20130019349A1 EPSPS MUTANTS 审中-公开
标题翻译： EPSPS MUTANT
公开(公告)号：US20130019349A1
公开(公告)日：2013-01-17
申请号：US13621662
申请日：2012-09-17
申请人： CIBUS US LLC , INCIMA US LLC
发明人： Greg F.W. Gocal , Mark E. Knuth , Peter R. Beetham
IPC分类号： A01H5/00
CPC分类号： C12N15/8275 , C12N9/1092 , C12N15/8213
摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. Additionally the present invention relates to mutant E. coli cells that contain mutated EPSPS genes.
摘要翻译：本发明涉及对膦酰甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的非转基因植物的生产。本发明还涉及重组型寡核苷酸在编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）的基因中的植物的染色体或附加型序列中进行所需的突变的用途。基本上保持野生型蛋白质的催化活性的突变蛋白质允许植物对膦酰基甲基甘氨酸家族的除草剂的抗性或耐受性增加，并允许植物，其器官的基本上正常的生长或发育，组织或细胞，与野生型植物相比，无论是否存在除草剂。此外，本发明涉及含有突变的EPSPS基因的突变大肠杆菌细胞。

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