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1. 发明授权

US09556106B2 Purification of cadaverine using high boiling point solvent 有权
标题翻译：使用高沸点溶剂净化尸胺
公开(公告)号：US09556106B2
公开(公告)日：2017-01-31
申请号：US14764109
申请日：2013-01-28
申请人： CATHAY R&D CENTER CO., LTD. , CATHAY INDUSTRIAL BIOTECH LTD.
发明人： Xiucai Liu , Charlie Liu , Duanfang Dai , Bingbing Qin , Naiqiang Li
IPC分类号： C07C209/84 , C12P13/00
CPC分类号： C07C209/84 , C12P13/001 , C07C211/09
摘要： One aspect of the invention relates to a method for the purification of cadaverine from an aqueous cadaverine composition comprising one or more involatile impurities, the method comprising obtaining cadaverine from evaporation or distillation of the aqueous cadaverine composition wherein one or more solvents are added to the evaporation/distillation system before the evaporation/distillation starts, during the evaporation/distillation and/or after the evaporation/distillation substantially stops when no more evaporation/distillation is observed; wherein the one or more solvents comprise at least one or more high boiling point (HBP) solvents. In certain embodiments, cadaverine may be evaporated/distilled at an unexpectedly lower heating temperature for an unexpectedly shorter heating time to provide a desired yield. Because cadaverine may decompose at high temperature, the unexpectedly lower heating temperature and unexpectedly shorter heating time decrease the undesired cadaverine decomposition. The methods disclosed herein provides unexpected high recovery rate (˜100%) in the evaporation/distillation of cadaverine at a larger scale at a lower heating temperature compared to conventional distillation methods. Thus, the method disclosed herein will be highly appreciated in the industrial production of cadaverine.
摘要翻译：本发明的一个方面涉及从包含一种或多种非挥发性杂质的水性尸胺组合物中纯化尸胺的方法，所述方法包括从蒸发或蒸馏水性尸胺组合物中获得尸胺，其中将一种或多种溶剂加入到蒸发中在蒸发/蒸馏开始之前，蒸发/蒸馏期间和/或蒸发/蒸馏之后蒸馏/蒸馏系统在不再观察到蒸发/蒸馏的情况下基本停止; 其中所述一种或多种溶剂包含至少一种或多种高沸点（HBP）溶剂。在某些实施方案中，可以在意想不到的较低加热温度下将尸胺蒸发/蒸馏，以期意外地更短的加热时间以提供所需的产率。因为尸体可能会在高温下分解，出人意料地降低加热温度，而且意外的加热时间会缩短，从而减少不希望的尸体分解。与常规蒸馏方法相比，本文所公开的方法在较低的加热温度下在较大规模的蒸发/蒸馏中提供了意想不到的高回收率（〜100％）。因此，本文公开的方法将在尸胺的工业生产中被高度赞赏。

2. 发明授权

US09234203B2 Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof 有权
标题翻译：稳定的重组表达质粒载体在hafnia alvei及其应用
公开(公告)号：US09234203B2
公开(公告)日：2016-01-12
申请号：US13795949
申请日：2013-03-12
申请人： CATHAY INDUSTRIAL BIOTECH LTD. , CATHAY R&D CENTER CO., LTD.
发明人： Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
IPC分类号： C12N15/74 , C12P13/00 , C08G69/26
CPC分类号： C12N9/88 , C07C211/09 , C08G69/26 , C12N15/74 , C12P13/00 , C12P13/001 , C12Y401/01018
摘要： One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.
摘要翻译：本公开的一个方面涉及包含编码抗毒素基因的多核苷酸的稳定的重组表达质粒载体，所述多核苷酸表达中和宿主细胞毒性多肽的多肽，所述毒性多肽由所述宿主细胞中的毒素基因表达，以及编码多肽表达产物的多核苷酸和稳定的重组表达质粒载体衍生自Hafnia alvei自主复制主链质粒。本公开的其它方面涉及用本文公开的稳定的重组表达质粒载体转化的转化体，使用本文公开的转化体生产生物基的尸胺的方法，以及通过本文公开的方法制备的生物基尸胺。本公开的另一方面涉及使用本文公开的生物基尸胺形成的聚酰胺及其组合物。本公开的另一方面涉及制备1,5-二异氰酸酯戊烷的方法，其包括使用本文公开的方法制备生物基尸胺，并将生物基尸胺转化为1,5-二异氰酸根戊烷。

3. 发明授权

US10221407B2 Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof 有权
公开(公告)号：US10221407B2
公开(公告)日：2019-03-05
申请号：US14992412
申请日：2016-01-11
申请人： CATHAY INDUSTRIAL BIOTECH LTD. , CATHAY R&D CENTER CO., LTD.
发明人： Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
IPC分类号： C12N9/88 , C12N15/74 , C07C211/09 , C12P13/00 , C08G69/26
摘要： One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.

4. 发明授权

US09546127B2 Purification of cadaverine 有权
标题翻译：尸体素的净化
公开(公告)号：US09546127B2
公开(公告)日：2017-01-17
申请号：US14764101
申请日：2013-01-28
申请人： CATHAY R&D CENTER CO., LTD. , CATHAY INDUSTRIAL BIOTECH LTD.
发明人： Xiucai Liu , Charlie Liu , Duanfang Dai , Bingbing Qin , Naiqiang Li
IPC分类号： C07C209/84 , C12P13/00
CPC分类号： C07C209/84 , C12P13/001 , C07C211/09
摘要： One aspect of the invention relates to a method for reducing salt content in an aqueous cadaverine salt composition comprising adjusting the pH of the aqueous cadaverine salt composition to provide a first composition comprising an aqueous cadaverine composition and a solid composition, wherein the pH of the aqueous cadaverine composition is at least about 12; and removing the solid composition from the first composition to provide the aqueous cadaverine composition. Another aspect of the invention relates to a method for the purification of cadaverine from an aqueous cadaverine salt composition using the salt-reducing method described herein; and obtaining cadaverine from the aqueous cadaverine composition.
摘要翻译：本发明的一个方面涉及一种降低含水尸胺盐组合物中盐含量的方法，包括调节含水尸胺盐组合物的pH以提供包含水性尸胺组合物和固体组合物的第一组合物，其中水性尸体组成至少约为12; 并从第一组合物中除去固体组合物以提供尸体尸体组合物。本发明的另一方面涉及使用本文所述的盐还原法从含水尸胺盐组合物中纯化尸胺的方法; 并从尸体尸体组合物中获得尸胺。

5. 发明授权

US10647976B2 Expression of polypeptides involved in lysine decarboxylation, and methods and applications thereof 有权
公开(公告)号：US10647976B2
公开(公告)日：2020-05-12
申请号：US15321800
申请日：2014-06-26
申请人： CATHAY R&D CENTER CO., LTD. , CATHAY INDUSTRIAL BIOTECH LTD.
发明人： Howard Chou , Naiqiang Li , Xiucai Liu
IPC分类号： C12N9/88 , C12N15/00 , C12N1/20 , C07H21/04 , C12P13/00 , C12N15/52
摘要： The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.

6. 发明申请

US20190040428A1 DECANEDIOIC ACID PRODUCED BY MICROBIAL FERMENTATION PROCESS AND PREPARATION METHOD THEREOF 审中-公开
公开(公告)号：US20190040428A1
公开(公告)日：2019-02-07
申请号：US16049119
申请日：2018-07-30
申请人： Cathay R&D Center Co., Ltd. , Cathay Industrial Biotech Ltd.
发明人： Min Xu , Yingli Hao , Chen Yang , Naiqiang Li , Xiucai Liu
IPC分类号： C12P7/64 , C07C55/20 , C08G69/26
摘要： The present invention provides a decanedioic acid produced by microbial fermentation process, in which the content of C10 aliphatic acid and C10 hydroxy aliphatic acid is maintained at a very low level. The present invention also provides a preparation method of the decanedioic acid and a polymer prepared by using the decanedioic acid as monomer. The decanedioic acid provided by the present invention is prepared by microbial fermentation process. The decanedioic acid product which is produced through the processes of microbial fermentation and separation has a higher purity, a higher thermal stability, and a lower impurity content. The decanedioic acid provided by the present invention could satisfy the requirements of high grade product of polyamide or polyester to produce polymer with excellent qualities. The preparation method of the decanedioic acid provided by the present invention which has many advantages of mild reaction conditions, environmental friendliness, high yield, and good product quality, may replace the chemical method to be used in industrial scale production of the decanedioic acid.

7. 发明授权

US09919996B2 Preparation of cadaverine 有权
公开(公告)号：US09919996B2
公开(公告)日：2018-03-20
申请号：US15406900
申请日：2017-01-16
申请人： CATHAY R&D CENTER CO., LTD. , CATHAY INDUSTRIAL BIOTECH LTD.
发明人： Xiucai Liu , Charlie Liu , Bingbing Qin , Naiqiang Li , XiaoMing Li
IPC分类号： C07C209/84 , C07C209/86
CPC分类号： C07C209/84 , C07C209/86 , C07C211/09
摘要： The invention relates to a method for preparing cadaverine comprising: (1) obtaining an aqueous cadaverine salt composition from a fermentation system; (2) adjusting the pH of the aqueous cadaverine salt composition to a pH of at least about 12 by adding an inorganic base composition to provide a first composition comprising an aqueous cadaverine composition having a pH of at least about 12 and a solid composition comprising one or more alkali metal sulfates; (3) subjecting the first composition to solid-liquid separation without organic solvent extraction to provide the aqueous cadaverine composition and the solid composition; (4) distilling or evaporating the aqueous cadaverine composition of step (3) without organic solvent extraction to provide purified cadaverine; (5) preparing a saturated solution of the alkali metal sulfate(s) from the solid composition of step (3) at a temperature of about 35-40° C., mixing the saturated solution with NH4HCO3, and subjecting the mixture to solid-liquid separation to provide Solution (I) and Solid (I); (6) adjusting the temperature of Solution (I) to about 60-80° C., and obtaining Solution (II) and Solid (II) after solid-liquid separation; (7) concentrating Solution (II) and obtaining Solution (III) and Solid (III) after solid-liquid separation; and (8) adding Solid (III) back to the fermentation system of step (1); wherein steps (1) to (8) are optionally repeated one or more times.

8. 发明授权

US09896409B2 Purification of cadaverine using high boiling point solvent 有权
公开(公告)号：US09896409B2
公开(公告)日：2018-02-20
申请号：US15418071
申请日：2017-01-27
申请人： CATHAY R&D CENTER CO., LTD. , CATHAY INDUSTRIAL BIOTECH LTD.
发明人： Xiucai Liu , Charlie Liu , Bingbing Qin , Naiqiang Li , XiaoMing Li
IPC分类号： C07C209/84
CPC分类号： C07C209/84 , C07C211/09
摘要： One aspect of the invention relates to a method for the purification of cadaverine from an aqueous cadaverine composition comprising one or more involatile impurities, the method comprising: a) distilling or evaporating the aqueous cadaverine composition wherein one or more solvents are added to the evaporation/distillation system before the evaporation/distillation starts, during the evaporation/distillation and/or after the evaporation/distillation substantially stops when no more evaporation/distillation is observed to produce purified cadaverine, wherein the evaporation or distillation is done at a heating temperature of about 80° C. to about 180° C. and under a pressure of 1 atm or lower, and the one or more solvents comprise at least one or more high boiling point (HBP) solvents having a boiling point of at least about 185° C. (1 atm); and b) recovering the one or more HBP solvents.

9. 发明申请

US20180002686A1 IMMOBILIZED CELL AND PREPARATION METHOD THEREOF 审中-公开
公开(公告)号：US20180002686A1
公开(公告)日：2018-01-04
申请号：US15548585
申请日：2015-02-03
申请人： Cathay R&D Center Co., Ltd. , Cathay Industrial Biotech Ltd.
发明人： Lijun Yu , Naiqiang Li , Charlie Liu , Xiucai Liu
IPC分类号： C12N11/08 , C12P13/00 , C12N9/88
CPC分类号： C12N11/08 , C12N9/88 , C12N11/02 , C12N11/04 , C12P13/001 , C12R1/01 , C12Y401/01018
摘要： Provided are a lysine decarboxylase immobilized cell and preparation method thereof.

10. 发明申请

US20170349705A1 NYLON SALT AND PREPARATION METHOD THEREFOR 审中-公开
公开(公告)号：US20170349705A1
公开(公告)日：2017-12-07
申请号：US15536906
申请日：2014-12-17
申请人： Cathay R&D Center Co., Ltd. , Cathay Industrial Biotech Ltd.
发明人： Chen Yang , Bingbing Qin , Yi Zheng , Charlie Liu , Naiqiang Li , Xiucai Liu
IPC分类号： C08G69/28
CPC分类号： C08G69/28 , C08G69/26
摘要： The present invention provides a method for preparing nylon salt, which comprises mixing 1,5-pentanediamine, water and dicarboxylic acid at a temperature of 65 to 120° C. to obtain a nylon salt, wherein the amount of the water is 2% to 12% by weight based on the total weight of the 1,5-pentanediamine and the dicarboxylic acid. The present invention also provides a nylon salt. According to the present method for preparing nylon salt, the incomplete reaction between the 1,5-pentanediamine and the dicarboxylic acid, which is caused by the nylon salt present during the preparation in a solid state, can be avoided; the reaction between the 1,5-pentanediamine and the dicarboxylic acid can be significantly speeded up, and the reaction time is accordingly shorten. Consequently, the content of diamine and dicarboxylic acid remained in the resulting nylon salt is very low. In addition, the final nylon salt product, which present in a solid state, makes it easier to be stored and transported.

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