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1. 发明授权

US5552282A Diagnosis of myotonic muscular dystrophy 失效
标题翻译：诊断肌强直性肌营养不良症
公开(公告)号：US5552282A
公开(公告)日：1996-09-03
申请号：US484044
申请日：1993-06-06
申请人： C. Thomas Caskey , Ying-Hui Fu , David L. Friedman , Antonio Pizzuti , Raymond G. Fenwick
发明人： C. Thomas Caskey , Ying-Hui Fu , David L. Friedman , Antonio Pizzuti , Raymond G. Fenwick
IPC分类号： C07K14/47 , C12Q1/68 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/6883 , C07K14/471 , C12Q2600/156
摘要： The present invention includes a DNA clone from the myotonic muscular dystrophy gene, a cosmid probe to the myotonic dystrophy site, as well as methods of detecting myotonic muscular dystrophy using RFLP. The method involves the steps of digesting DNA from an individual to be tested with a restriction endonuclease and detecting the restriction fragment length polymorphism with hybridization to probes within the myotonic muscular locus and southern blot analysis. Alternatively, the myotonic muscular dystrophy gene can be measured by determining the amount of mRNA or measuring the amount of protein with an antibody. Further, the myotonic muscular dystrophy gene defect can be detected using either fluorescence in situ hybridization or pulsed field gel electrophoresis using the probes described herein.
摘要翻译：本发明包括来自肌强直性营养不良基因的DNA克隆，对肌强直营养不良位点的粘粒探针，以及使用RFLP检测肌强直性营养不良的方法。该方法包括用限制性内切核酸酶消化来自待测个体的DNA的步骤，并通过与强直肌肌肉轨迹内的探针杂交和Southern印迹分析检测限制性片段长度多态性。或者，可以通过测定mRNA的量或用抗体测量蛋白质的量来测量肌强直性肌营养不良基因。此外，使用本文所述的探针，可以使用荧光原位杂交或脉冲场凝胶电泳检测肌强直性肌营养不良基因缺陷。

2. 发明授权

US06459994B1 Methods for computer-assisted isolation of proteins 失效
标题翻译：计算机辅助分离蛋白质的方法
公开(公告)号：US06459994B1
公开(公告)日：2002-10-01
申请号：US09724383
申请日：2000-11-28
申请人： Rajesh Bhikhu Parekh , James Alexander Bruce , Robin Philp , Lida H. Kimmel , David L. Friedman
发明人： Rajesh Bhikhu Parekh , James Alexander Bruce , Robin Philp , Lida H. Kimmel , David L. Friedman
IPC分类号： G01N3348
CPC分类号： C07K1/285 , G01N2030/027
摘要： The present invention is directed to efficient, computer-assisted methods and apparatus for identifying, selecting and characterizing biomolecules in a biological sample. According to the invention, a biological sample is first treated to isolate biomolecules of interest, and a two-dimensional array is then generated by separating the biomolecules present in a complex mixture. The invention provides a computer-generated digital profile representing the identity and relative abundance of a plurality of biomolecules detected in the two-dimensional array, thereby permitting computer-mediated comparison of profiles from multiple biological samples. This automatable technology for screening biological samples and comparing their profiles permits rapid and efficient identification of individual biomolecules whose presence, absence or altered expression is associated with a disease or condition of interest.
摘要翻译：本发明涉及用于识别，选择和表征生物样品中生物分子的有效的计算机辅助方法和装置。根据本发明，首先处理生物样品以分离目标生物分子，然后通过分离存在于复杂混合物中的生物分子来产生二维阵列。本发明提供了计算机生成的数字简档，其表示在二维阵列中检测到的多个生物分子的身份和相对丰度，从而允许计算机介导的来自多个生物样品的分布的比较。用于筛选生物样品并比较其特征的这种可自动化技术允许快速和有效地鉴定其存在，不存在或改变的表达与感兴趣的疾病或病症相关联的个体生物分子。

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