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    • 7. 发明申请
    • Method of cryopreservation of tissues by vitrification
    • 通过玻璃化冷冻保存组织的方法
    • US20050100876A1
    • 2005-05-12
    • US10646802
    • 2003-08-25
    • Bijan KhirabadiYing SongKelvin Brockbank
    • Bijan KhirabadiYing SongKelvin Brockbank
    • A01N1/02A61K35/12A61K35/44
    • A01N1/02A01N1/0221A01N1/0284A61K35/44
    • A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than −15° C. to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5-100° C. per minute to a temperature between −80° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30° C. per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20-40° C. per minute to a temperature between −80° C. and the glass transition temperature; further warming the tissue or organ at a rate greater than 80° C. per minute to a temperature above −75° C.; and reducing the concentration of the cryoprotectant. Tissues or organs treated in this manner exhibit near normal functions, for example, blood vessels exhibit near normal smooth muscle contractility and normal graft functions.
    • 一种组织或器官的玻璃化方法包括将组织或器官在高于-15℃的温度下浸入浓度越来越高的冷冻保护剂浓度至足以进行玻璃化的冷冻保护剂浓度; 以2.5-100℃/分钟的平均速率将组织或器官冷却至-80℃和玻璃化转变温度之间的温度; 并且将组织或器官进一步以低于30℃/分钟的平均速率冷却到低于玻璃化转变温度的温度以使组织或器官玻璃化。 在玻璃化组织或器官被储存之后,组织或器官可以通过将组织或器官以20-40℃/分钟的平均速率加热到-80℃和-80℃之间的温度而从玻璃化中除去 玻璃化转变温度; 以大于80℃/分钟的速度将组织或器官进一步升温至-75℃以上的温度; 并降低冷冻保护剂的浓度。 以这种方式处理的组织或器官表现出接近正常的功能,例如,血管表现出接近正常的平滑肌收缩和正常的移植功能。