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    • 2. 发明授权
    • Alleles of the rel gene from coryneform bacteria
    • 来自棒状细菌的rel基因的等位基因
    • US07754446B2
    • 2010-07-13
    • US11873073
    • 2007-10-16
    • Brigitte BatheCaroline KreutzerGeorg Thierbach
    • Brigitte BatheCaroline KreutzerGeorg Thierbach
    • C12P1/00
    • C12P13/08A23L33/135A23L33/175C12N9/1235C12P13/04
    • An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
    • 棒状细菌的分离突变体,其包含编码具有GTP-焦磷酸激酶活性的多肽的基因,其中所述多肽包含氨基酸序列,其中除了L-脯氨酸以外的蛋白原氨基酸之一存在于位置38或相应的 或相当的地位。 此外,描述了编码具有GTP焦磷酸激酶酶活性的多肽的分离的多核苷酸,包含分离的多核苷酸的载体,包含载体的重组微生物和制备重组棒状杆菌型细菌的方法。 还描述了过表达GTP焦磷酸激酶的方法,制备L-氨基酸的方法,包含L-赖氨酸的L-赖氨酸和包含L-色氨酸的饲料。