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1. 发明授权

US06300483B1 Compositions inducing cleavage of RNA motifs 失效
标题翻译：诱导RNA基序切割的组合物
公开(公告)号：US06300483B1
公开(公告)日：2001-10-09
申请号：US08879078
申请日：1997-06-19
申请人： János Ludwig , Brian S. Sproat
发明人： János Ludwig , Brian S. Sproat
IPC分类号： C07H2102
CPC分类号： C12N15/113 , A61K38/00 , C12N15/1131 , C12N15/1133 , C12N15/1135 , C12N15/1138 , C12N2310/111 , C12N2310/121 , C12N2310/317 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/3523
摘要： Disclosed are compositions inducing cleavage of an RNA substrate, as well as their use for inducing cleavage of RNA substrates in vitro and in vivo. The compositions contain part of an active center, with the other part of the active center provided by the RNA substrate. The subunits of the active center region of the compositions are nucleotides and/or nucleotide analogues. The disclosed compositions also have flanking regions contributing to the formation of a specific hybridization with an RNA substrate. Preferred compositions form, in combination with an RNA substrate, a structure resembling a hammerhead structure. The active center of the disclosed compositions is characterized by the presence of I15.1 which allows cleavage of RNA substrates having C16.1.
摘要翻译：公开了诱导RNA底物切割的组合物，以及它们在体外和体内诱导RNA底物切割的用途。组合物含有活性中心的一部分，活性中心的另一部分由RNA底物提供。组合物的活性中心区域的亚基是核苷酸和/或核苷酸类似物。所公开的组合物还具有有助于与RNA底物形成特异性杂交的侧翼区域。优选的组合物与RNA底物组合形成类似锤头结构的结构。所公开组合物的活性中心的特征在于I15.1的存在，其允许切割具有C16.1的RNA底物。

2. 发明授权

US06620926B2 Purification of oligomers 失效
公开(公告)号：US06620926B2
公开(公告)日：2003-09-16
申请号：US09908042
申请日：2001-07-18
申请人： Brian S. Sproat
发明人： Brian S. Sproat
IPC分类号： C07H2100
CPC分类号： C07H19/06 , C07F7/1804 , C07H21/00
摘要： Compositions and methods are disclosed which facilitate purification of oligomers and other compounds. The disclosed compositions are silyl compositions that can be directly coupled, or coupled through a linking group, to a compound of interest, preferably to an oligomer at the end of oligomer synthesis. The silicon atom includes between one and three sidechains that function as capture tags. In one embodiment, the capture tags are lipophilic, which allows a derivatized oligomer to be separated from failure sequences by reverse phase chromatography. In another embodiment, the capture tags are compounds with a known affinity for other compounds, which other compounds are preferably associated with a solid support to allow chromatographic separation. Examples include haptens, antibodies, and ligands. Biotin, which can bind to or interact with a streptavidin-bound solid support, is a preferred capture tag of this type.

3. 发明授权

US06921818B2 Purification of oligomers 有权
标题翻译：低聚物的纯化
公开(公告)号：US06921818B2
公开(公告)日：2005-07-26
申请号：US10627934
申请日：2003-07-25
申请人： Brian S. Sproat
发明人： Brian S. Sproat
IPC分类号： C07H23/00 , C07F7/08 , C07F7/18 , C07F9/24 , C07H19/06 , C07H21/00 , C07H19/04 , C12Q1/68 , C01B33/08
CPC分类号： C07H19/06 , C07F7/1804 , C07H21/00
摘要： Compositions and methods are disclosed which facilitate purification of oligomers and other compounds. The disclosed compositions are silyl compositions that can be directly coupled, or coupled through a linking group, to a compound of interest, preferably to an oligomer at the end of oligomer synthesis. The silicon atom includes between one and three sidechains that function as capture tags. In one embodiment, the capture tags are lipophilic, which allows a derivatized oligomer to be separated from failure sequences by reverse phase chromatography. In another embodiment, the capture tags are compounds with a known affinity for other compounds, which other compounds are preferably associated with a solid support to allow chromatographic separation. Examples include haptens, antibodies, and ligands. Biotin, which can bind to or interact with a streptavidin-bound solid support, is a preferred capture tag of this type.
摘要翻译：公开了有助于纯化低聚物和其它化合物的组合物和方法。所公开的组合物是甲硅烷基组合物，其可以在低聚物合成的末端直接偶联或通过连接基团偶联到感兴趣的化合物，优选至低聚物。硅原子包括用作捕获标签的一个和三个侧链之间。在一个实施方案中，捕获标签是亲脂性的，其允许衍生的寡聚体通过反相色谱与失效序列分离。在另一个实施方案中，捕获标签是对其他化合物具有已知亲和力的化合物，其它化合物优选与固体支持物相关联以允许色谱分离。实例包括半抗原，抗体和配体。生物素可以与链亲和素结合的固体支持物结合或相互作用，是这种类型的优选捕获标签。

4. 发明授权

US06410225B1 Purification of oligomers 失效
标题翻译：低聚物的纯化
公开(公告)号：US06410225B1
公开(公告)日：2002-06-25
申请号：US08883712
申请日：1997-06-27
申请人： Brian S. Sproat
发明人： Brian S. Sproat
IPC分类号： C12Q168
CPC分类号： C07H19/06 , C07F7/1804 , C07H21/00
摘要： Compositions and methods are disclosed which facilitate purification of oligomers and other compounds. The disclosed compositions are silyl compositions that can be directly coupled, or coupled through a linking group, to a compound of interest, preferably to an oligomer at the end of oligomer synthesis. The silicon atom includes between one and three sidechains that function as capture tags. In one embodiment, the capture tags are lipophilic, which allows a derivatized oligomer to be separated from failure sequences by reverse phase chromatography. In another embodiment, the capture tags are compounds with a known affinity for other compounds, which other compounds are preferably associated with a solid support to allow chromatographic separation. Examples include haptens, antibodies, and ligands. Biotin, which can bind to or interact with a streptavidin-bound solid support, is a preferred capture tag of this type.
摘要翻译：公开了有助于纯化低聚物和其它化合物的组合物和方法。所公开的组合物是甲硅烷基组合物，其可以在低聚物合成的末端直接偶联或通过连接基团偶联到感兴趣的化合物，优选至低聚物。硅原子包括用作捕获标签的一个和三个侧链之间。在一个实施方案中，捕获标签是亲脂性的，其允许衍生的寡聚体通过反相色谱与失效序列分离。在另一个实施方案中，捕获标签是对其他化合物具有已知亲和力的化合物，其它化合物优选与固体支持物相关联以允许色谱分离。实例包括半抗原，抗体和配体。生物素可以与链亲和素结合的固体支持物结合或相互作用，是这种类型的优选捕获标签。

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