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    • 9. 发明申请
    • Methods of using glucan synthase pathway reporter genes to screen for antifungal compounds
    • 使用葡聚糖合酶通路报道基因筛选抗真菌化合物的方法
    • US20050084848A1
    • 2005-04-21
    • US10324035
    • 2002-12-19
    • John Phillips
    • John Phillips
    • C12Q1/68
    • C12Q1/6897C12Q1/6895C12Q2600/158
    • The present invention relates to methods of using nucleotide sequences from the promoter region of at least one of seven S. cerevisiae genes whose expression is an indicator of the inhibition or modulation of the glucan synthase pathway in S. cerevisiae. This invention envisions using at least one target polynucleotide sequence, each target polynucleotide sequence being operably linked to the promoter region of one of the seven glucan synthase pathway reporter genes, to screen chemical libraries and natural products for compounds which can be used as antifungal agents for use against a variety of fungal pathogens. This invention also envisions using the methods of the invention to assay the efficacy of and/or specificity of antifungal agents, and/or to monitor the activity of the glucan synthase pathway.
    • 本发明涉及使用七个酿酒酵母基因中至少一个的启动子区域的核苷酸序列的方法,其表达是酿酒酵母中葡聚糖合成酶途径的抑制或调节的指标。 本发明设想使用至少一个靶多核苷酸序列,每个靶多核苷酸序列可操作地连接到七个葡聚糖合成酶途径报道基因之一的启动子区域,以筛选可用作抗真菌剂的化合物的化学文库和天然产物 使用各种真菌病原体。 本发明还设想使用本发明的方法来测定抗真菌剂的功效和/或特异性,和/或监测葡聚糖合成酶途径的活性。