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1. 发明申请

US20150029595A1 Mechanical Zoom Imaging Apparatus 有权
标题翻译：机械变焦成像设备
公开(公告)号：US20150029595A1
公开(公告)日：2015-01-29
申请号：US14444709
申请日：2014-07-28
申请人： Bio-Rad Laboratories, Inc.
发明人： Steve Swihart , Evan Thrush , Evelio Perez , Kevin McDonald
IPC分类号： G02B15/14
CPC分类号： G02B15/14
摘要： An imaging assembly for the viewing, imaging, and analysis of chemiluminescent or bioluminescent samples in gels or other substrates, in which an adjustable camera and lens module having a prime or fixed lens or a focusing lens is moved to change the field of view by shifting the focal plane of the camera and lens module. The imaging assembly can also include a mirror to bend or fold the optical path between the camera and lens module and the target area having a sample, in which the mirror can move in the same vertical direction as the camera and lens module. Further, the camera and lens module can be configured to more move in a diagonal direction relative to the location of the imaging target area. The imaging assembly can further have a duct system adaptable to adjust with the movement of the camera and lens module.
摘要翻译：用于在凝胶或其它基底中观察，成像和分析化学发光或生物发光样品的成像组件，其中具有主要或固定透镜或聚焦透镜的可调节照相机和透镜模块移动以通过移动来改变视场相机和镜头模块的焦平面。成像组件还可以包括用于弯曲或折叠相机和透镜模块之间的光路的反射镜和具有样品的目标区域，其中反射镜可以在与相机和透镜模块相同的垂直方向上移动。此外，相机和透镜模块可以被配置为相对于成像目标区域的位置在对角线方向上更多地移动。成像组件还可以具有适于根据相机和透镜模块的运动进行调节的管道系统。

2. 发明授权

US09618733B2 Mechanical zoom imaging apparatus 有权
公开(公告)号：US09618733B2
公开(公告)日：2017-04-11
申请号：US14444709
申请日：2014-07-28
申请人： Bio-Rad Laboratories, Inc.
发明人： Steve Swihart , Evan Thrush , Evelio Perez , Kevin McDonald
IPC分类号： G02B15/14
CPC分类号： G02B15/14
摘要： An imaging assembly for the viewing, imaging, and analysis of chemiluminescent or bioluminescent samples in gels or other substrates, in which an adjustable camera and lens module having a prime or fixed lens or a focusing lens is moved to change the field of view by shifting the focal plane of the camera and lens module. The imaging assembly can also include a mirror to bend or fold the optical path between the camera and lens module and the target area having a sample, in which the mirror can move in the same vertical direction as the camera and lens module. Further, the camera and lens module can be configured to more move in a diagonal direction relative to the location of the imaging target area. The imaging assembly can further have a duct system adaptable to adjust with the movement of the camera and lens module.

3. 发明授权

US10539556B2 Stain-free protein quantification and normalization 有权
公开(公告)号：US10539556B2
公开(公告)日：2020-01-21
申请号：US16158853
申请日：2018-10-12
申请人： Bio-Rad Laboratories, Inc.
发明人： Steve Freeby , Ning Liu , Kevin McDonald , Aran Paulus , Anton Posch
IPC分类号： G01N33/53 , G16B99/00 , G01N33/68 , G06T7/00 , G06T11/60
摘要： Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

4. 发明授权

US10119961B2 Stain-free protein quantification and normalization 有权
公开(公告)号：US10119961B2
公开(公告)日：2018-11-06
申请号：US15293154
申请日：2016-10-13
申请人： Bio-Rad Laboratories, Inc.
发明人： Steve Freeby , Ning Liu , Kevin McDonald , Aran Paulus , Anton Posch
IPC分类号： G01N33/53 , G01N33/68 , G06F19/10 , G06T7/00 , G06T11/60
摘要： Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

5. 发明授权

US09058648B2 Image acquisition for chemiluminescent samples 有权
标题翻译：化学发光样品的图像采集
公开(公告)号：US09058648B2
公开(公告)日：2015-06-16
申请号：US13803562
申请日：2013-03-14
申请人： Bio-Rad Laboratories, Inc.
发明人： Keith Kotchou , Kevin McDonald , James Lee
IPC分类号： G06K9/00 , G06T7/00 , G01N21/76 , G06T5/00 , G06T5/50 , H04N5/235
CPC分类号： G06T7/0012 , G01N21/76 , G06T5/008 , G06T5/50 , G06T2200/21 , G06T2207/10064 , G06T2207/10144 , G06T2207/20012 , G06T2207/20208 , G06T2207/30004 , H04N5/2351 , H04N5/2355
摘要： An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.
摘要翻译：用于检测样品中化学发光的图像系统使用高度装箱的短曝光初始图像来计算样品的最终图像的曝光时间。在曝光时间的计算之后，拍摄至少两个最终图像，其中去除了饱和像素，并在第一图像中用来自第二图像的相应的不饱和像素替换。调整相应的像素以反映第一和第二图像之间的不同强度水平，并且第一图像成为反映检测到的化学发光的最终图像。

6. 发明申请

US20140226161A1 DENSITOMETER STEP TABLET 审中-公开
标题翻译：密度计步骤片
公开(公告)号：US20140226161A1
公开(公告)日：2014-08-14
申请号：US14179148
申请日：2014-02-12
申请人： Bio-Rad Laboratories, Inc.
发明人： Jeff Blitstein , Keith Kotchou , Kevin McDonald
IPC分类号： G01N21/59 , G01N21/55
CPC分类号： G01N21/5907 , G01N21/55
摘要： The present invention provides a transmissive density and/or reflective density reference step tablet and methods of using thereof for calibrating a densitometer or the like.
摘要翻译：本发明提供透射密度和/或反射密度参考步骤片及其用于校准密度计等的方法。

7. 发明申请

US20130288388A1 STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION 审中-公开
标题翻译：无蛋白质定量和正常化
公开(公告)号：US20130288388A1
公开(公告)日：2013-10-31
申请号：US13870710
申请日：2013-04-25
申请人： BIO-RAD LABORATORIES, INC., LSG - LSD DIVISION
发明人： Steve Freeby , Ning Liu , Kevin McDonald , Aran Paulus , Anton Posch
IPC分类号： G01N33/53
CPC分类号： G01N33/53 , G01N33/6827 , G06F19/10 , G06T7/0012 , G06T11/60 , G06T2207/30004
摘要： Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.
摘要翻译：本文公开了使用卤代烷基化色氨酸荧光进行蛋白质定量和归一化的方法。使用与色氨酸残基反应以形成荧光产物的卤素取代的有机化合物（即卤代烷）处理复合蛋白质样品，即每种含有来自不同来源的不具有共同蛋白质谱的1,000个或更多个不同蛋白质的样品。然后使用紫外光照射样品，并对每个样品中所有蛋白质产生的荧光发射进行检测和定量，以获得各种样品中总蛋白质含量的比较值。这样获得的值被发现是比较总蛋白质含量的有效指示，尽管事实上，任何单个样品中的各种蛋白质之间的色氨酸水平变化很大，并且样品由于其起源的多样性而趋于彼此不同在它们所含的蛋白质的身份和相对量中。蛋白质样品也通过使用每个样品中的总蛋白的无染色检测或每个样品中的子样品检测来校准样品稀释度，样品加载和蛋白质转移不一致性的差异。

8. 发明申请

US20130243283A1 IMAGE ACQUISITION FOR CHEMILUMINESCENT SAMPLES 有权
标题翻译：图像采集用于显微镜样品
公开(公告)号：US20130243283A1
公开(公告)日：2013-09-19
申请号：US13803562
申请日：2013-03-14
申请人： BIO-RAD LABORATORIES, INC.
发明人： Keith Kotchou , Kevin McDonald , James Lee
IPC分类号： G06T7/00
CPC分类号： G06T7/0012 , G01N21/76 , G06T5/008 , G06T5/50 , G06T2200/21 , G06T2207/10064 , G06T2207/10144 , G06T2207/20012 , G06T2207/20208 , G06T2207/30004 , H04N5/2351 , H04N5/2355
摘要： An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.
摘要翻译：用于检测样品中化学发光的图像系统使用高度装箱的短曝光初始图像来计算样品的最终图像的曝光时间。在曝光时间的计算之后，拍摄至少两个最终图像，其中去除了饱和像素，并在第一图像中用来自第二图像的相应的不饱和像素替换。调整相应的像素以反映第一和第二图像之间的不同强度水平，并且第一图像成为反映检测到的化学发光的最终图像。

9. 发明申请

US20210356397A1 ILLUMINATOR SYSTEMS HAVING LIGHT EMITTING DIODES WITH UV ACTIVATION 有权
公开(公告)号：US20210356397A1
公开(公告)日：2021-11-18
申请号：US17314406
申请日：2021-05-07
申请人： BIO-RAD LABORATORIES, INC.
发明人： Stephen Swihart , Evan Thrush , Kevin McDonald
IPC分类号： G01N21/64 , H05B45/10 , H05B45/20 , G01N21/31 , G01N21/33
摘要： An imaging system including an illuminator apparatus or an epi-illumination apparatus that has LEDs for illuminations is provided for stain-free gel activation and fluorescent sample visualization. The illuminator apparatus includes a housing, a light source array disposed on at least one side surface of the housing and including at least one plurality of LEDs having each LED individually operable to output light of a predetermined color within a range of wavelengths, and a controller for controlling ranges of operational parameters of the at least one plurality of LEDs. The light emitted from the light source array incidents upon a sample having a gel that includes a product of UV light induced reaction between tryptophan and a haloalkane and the light emitted from the light source array includes ultraviolet (UV) light to excite a fluorescent response of the sample.

10. 发明授权

US09933565B2 Contact imaging devices for fluorescence applications 有权
公开(公告)号：US09933565B2
公开(公告)日：2018-04-03
申请号：US15211728
申请日：2016-07-15
申请人： Bio-Rad Laboratories, Inc.
发明人： Steve Swihart , Evan Thrush , Yochanan Uri , Boaz Ran , Itay Barak , Kevin McDonald
IPC分类号： G02B6/08 , G02B5/22 , G01N21/64 , G02B5/28 , G01N27/447
CPC分类号： G02B6/08 , G01N21/6454 , G01N27/44721 , G01N2021/6484 , G02B5/22 , G02B5/28
摘要： Devices, systems, methods, and kits for contact imaging are provided. A contact imaging device includes an imaging sensor, a fixed fiber faceplate mechanically coupled to the imaging sensor, and an optical filtering layer mechanically coupled to the fixed fiber faceplate. The optical filtering layer can include an interference filter, an absorptive filter, and/or a removable fiber faceplate. The contact imaging device can be used to image fluorescent samples by filtering out excitation light on the basis of wavelength and/or angle of incidence.

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