专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US6017707A Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
标题翻译：用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：US6017707A
公开(公告)日：2000-01-25
申请号：US870730
申请日：1997-06-06
申请人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
IPC分类号： C08G81/02 , C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译：检测目的核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。所述方法包括基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元，摩尔当量，每摩尔聚合物。这样的试剂能够获得信号放大，从而降低灵敏度阈值。特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

2. 发明授权

US5695936A Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
标题翻译：用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：US5695936A
公开(公告)日：1997-12-09
申请号：US433505
申请日：1995-06-14
申请人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
IPC分类号： C08G81/02 , C12Q1/68
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译： PCT No.PCT / FR94 / 01084第 371 1995年6月14日第 102（e）1995年6月14日PCT PCT 1994年9月15日PCT公布。 WO95 / 08000 PCT出版物 1995年3月23日的方法检测目标核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。所述方法包括使用基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元，摩尔当量，每摩尔聚合物。这样的试剂能够获得信号放大，从而降低灵敏度阈值。特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

3. 发明授权

US5723344A Device for the capture of target molecules, and capturing process using the device 失效
标题翻译：用于捕获目标分子的装置，以及使用该装置的捕获过程
公开(公告)号：US5723344A
公开(公告)日：1998-03-03
申请号：US264996
申请日：1994-06-24
申请人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Claude Mabilat , Philippe Cros , Bernard Mandrand , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
IPC分类号： G01N33/543 , C12Q1/68 , G01N33/544
CPC分类号： G01N33/54353 , G01N33/54366 , Y10S435/975 , Y10S436/807
摘要： Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption. When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.
摘要翻译：用于捕获靶分子以用于检测和/或测定靶分子的装置，包括其上固定有配体的固体支持体，所述配体以由聚合物与多个共价键共价偶联得到的缀合物形式提供的配体分子。聚合物是N-乙烯基吡咯烷酮共聚物，并且通过吸附将缀合物固定在固体载体上。当配体能够与目标物形成复合物时，该装置对于给定的靶标是特异性的。此外，当该装置另外包含能够与配体一起形成络合物的双功能试剂，另一方面，另外，与靶标配位的配体固定的载体构成通用捕获系统。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式