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1. 再颁专利

USRE39006E1 Method of detecting hematopoietic progenitor cells 有权
标题翻译：检测造血祖细胞的方法
公开(公告)号：USRE39006E1
公开(公告)日：2006-03-07
申请号：US10704463
申请日：2003-11-07
申请人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
发明人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
IPC分类号： C12Q1/04
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
摘要翻译：用于检测和/或计数造血祖细胞外观的改进方法，其包括以下步骤：将血液样品与能够检测未成熟细胞的试剂混合而不使用任何免疫技术，使用粒子分析仪获得关于如此处理的样品的细胞信息; 构建细胞分布轮廓，将轮廓的一部分描绘为其中存在至少一个造血祖细胞亚类的区域，并对该区域内的细胞进行计数。该方法能够以简单和积极的方式检测造血祖细胞的外观。

2. 发明授权

US5830701A Method of detecting hematopoietic progenitor cells 失效
公开(公告)号：US5830701A
公开(公告)日：1998-11-03
申请号：US829239
申请日：1997-03-28
申请人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi
发明人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi
IPC分类号： G01N33/48 , C12N5/08 , G01N15/00 , G01N15/14 , G01N33/49 , G01N33/50 , C12Q1/04 , C12Q1/06 , G01N21/76
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.

3. 发明授权

US5413938A Reagent for measuring immature leukocytes 失效
标题翻译：用于测量未成熟白细胞的试剂
公开(公告)号：US5413938A
公开(公告)日：1995-05-09
申请号：US214248
申请日：1994-03-17
申请人： Yukio Tsujino , Takashi Morikawa , Yukio Hamaguchi
发明人： Yukio Tsujino , Takashi Morikawa , Yukio Hamaguchi
IPC分类号： G01N33/48 , G01N33/49 , G01N33/50 , G01N33/52
CPC分类号： G01N33/5094 , G01N15/12 , G01N15/1459 , G01N2015/1006 , G01N2015/1402 , Y10S436/825 , Y10T436/25125 , Y10T436/2525
摘要： A reagent for measuring immature leukocytes which comprises: (1) a polyoxyethylene-based nonionic surfactant having the general formula (I) in a sufficient amount capable of fixing cytoplasms and cell membranes of immature leukocytes:R.sub.1 --R.sub.2 --(CH.sub.2 CH.sub.2 O ).sub.n --H (I)where R.sub.1 is an alkyl, alkenyl or alkynyl group having 10 to 25 carbon atoms; R.sub.2 is --O--, --(C.sub.6 H.sub.4)--O-- or --COO--; and n is an integer of 10 to 40, (2) a solubilizing agent in a sufficient amount capable of damaging cell membranes of blood cells other than immature leukocytes and shrinking them, (3) an amino acid in a sufficient amount capable of stabilizing cytoplasm and cell membrane of immature leukocytes, and (4) an aqueous medium, which adjusts pH value in the range of 5.0 to 9.0, osmolarity in the range of 150 to 600 mOsm/kg and electric conductivity in the range of 6.0 to 9.0 mS/cm, respectively.
摘要翻译： 1.一种用于测定未成熟白细胞的试剂，其特征在于：（1）具有通式（I）的聚氧乙烯类非离子表面活性剂，其量足以固定未成熟白细胞的细胞质和细胞膜：R1-R2-（CH2CH2O）nH ）其中R1是具有10-25个碳原子的烷基，烯基或炔基; R2是-O - ， - （C6H4）-O-或-COO-; n为10〜40的整数，（2）能够损伤除未成熟白细胞以外的血细胞的细胞膜并使其收缩的足够量的增溶剂，（3）足以稳定细胞质的氨基酸和未成熟白细胞的细胞膜，和（4）调节pH值在5.0至9.0范围内的渗透压，150至600mOsm / kg范围内的渗透压和6.0至9.0mS / 厘米。

4. 发明授权

US5389549A Method for classifying leukocytes and a reagent used therefor 失效
标题翻译：白细胞分类方法和用于其的试剂
公开(公告)号：US5389549A
公开(公告)日：1995-02-14
申请号：US964347
申请日：1992-10-21
申请人： Yukio Hamaguchi , Kenji Ito , Yukio Tsujino , Kazuhiro Moriyama , Ikuya Takenaka , Takashi Morikawa , Hitomi Ohmi
发明人： Yukio Hamaguchi , Kenji Ito , Yukio Tsujino , Kazuhiro Moriyama , Ikuya Takenaka , Takashi Morikawa , Hitomi Ohmi
IPC分类号： G01N15/14 , G01N33/50 , G01N31/00
CPC分类号： G01N33/5094 , G01N2015/1488 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： Reagents for enabling leukocytes to be classified and counted lyse erythrocytes and act on leukocytes to stabilize them are disclosed. The reagents contain polyoxyethylene-based surfactants, and may contain hyperosmotic or hypoosmotic agents and solubilizing agents. One embodiment of the invention contains surfactants selected from the anionic and nonionic polyoxyethylene-based surfactants having 18-30 repeating oxyethylene units per molecule. A second embodiment of the invention contains nonionic polyoxyethylene surfactants only, having 20-100 repeating oxyethylene units per molecule, does not employ either saponin or quaternary ammonium compounds, and provides for adjustment of the pH of the resulting solutions. The invention allows leukocytes to be classified into as many as five types, each type to be quantified, and enables the detection of abnormal cells, when used in conjunction with particulate analysis flow cytometers employing the RF method and the DC method.
摘要翻译：公开了使白细胞分类和计数裂解红细胞并作用于白细胞以使其稳定的试剂。试剂含有聚氧乙烯基表面活性剂，并且可能含有高渗或低渗透剂和增溶剂。本发明的一个实施方案包含选自阴离子和非离子聚氧乙烯基表面活性剂的表面活性剂，每分子具有18-30个重复氧乙烯单元。本发明的第二个实施方案仅含有非离子聚氧乙烯表面活性剂，每分子具有20-100个重复氧化乙烯单元，不使用皂苷或季铵化合物，并且用于调节所得溶液的pH。本发明允许将白细胞分为多种五种类型，每种类型被定量，并且当与使用RF方法和DC方法的颗粒分析流式细胞仪结合使用时，能够检测异常细胞。

5. 发明授权

US5116539A Reagent and method for measuring leukocytes and hemoglobin in blood 失效
标题翻译：用于测量血液中白蛋白和血红蛋白的试剂和方法
公开(公告)号：US5116539A
公开(公告)日：1992-05-26
申请号：US266860
申请日：1988-11-03
申请人： Yukio Hamaguchi , Yukio Tsujino
发明人： Yukio Hamaguchi , Yukio Tsujino
IPC分类号： G01N33/48 , G01N33/49 , G01N33/50 , G01N33/72
CPC分类号： G01N33/721 , G01N33/5094 , Y10S516/01 , Y10T436/101666
摘要： A reagent for and method of measuring leukocytes or hemoglobin or both in blood using a reagent which is water-soluble mixture containing:(a) a polyoxyethylene-based nonionic surfactant represented by the formula:R.sub.1 --R.sub.2 (CH.sub.2 CH.sub.2 O).sub.n --H where R.sub.1 is an alkyl, alkenyl or alkynyl group having 6 to 24 carbon atoms, R.sub.2 is --O--, ##STR1## or --COO--, and n is an integer of 6 to 50, and (b) a buffer to adjust the pH of the solution within the range of 3-11 is disclosed. Measurements of hemoglobin in blood samples are of extreme importance to clinical diagnosis of such diseases as leukemia and anemia. Measurements of eosinophils, which are in the category of leukocytes, are important to the diagnosis of allergic conditions.

6. 发明申请

US20110250588A1 METHOD FOR DETECTING CANCER CELLS IN BLOOD SAMPLE 审中-公开
标题翻译：检测血液样品中癌细胞的方法
公开(公告)号：US20110250588A1
公开(公告)日：2011-10-13
申请号：US13140723
申请日：2009-12-14
申请人： Jun Sato , Akinori Masago , Yukio Tsujino
发明人： Jun Sato , Akinori Masago , Yukio Tsujino
IPC分类号： C12Q1/70
CPC分类号： G01N33/574
摘要： A method for detecting cancer cells in a blood sample is provided comprising the steps of proliferating an oncolytic virus at least in the cancer cells by incubating the blood sample being suspected to contain the cancer cells with the oncolytic virus; mixing the blood sample obtained from the proliferating step with a fixing agent and a nonionic surfactant; and detecting the cancer cells in the blood sample obtained from the mixing step, in which the oncolytic virus has been proliferated.
摘要翻译：提供了一种用于检测血液样品中癌细胞的方法，包括以下步骤：至少在癌细胞中通过将涉嫌含有癌细胞的血液样品与溶瘤病毒一起孵育来增殖溶瘤病毒; 将从增殖步骤获得的血液样品与固定剂和非离子表面活性剂混合; 并检测从其中已经增殖了溶瘤病毒的混合步骤获得的血液样品中的癌细胞。

7. 发明申请

US20110124005A1 REAGENT FOR DETECTING ABNORMAL CELL IN CERVIX OF UTERUS, AND METHOD FOR DETECTING ABNORMAL CELL IN CERVIX OF UTERUS BY USING SAME 审中-公开
标题翻译：用于检测子宫颈异常细胞的试剂，以及通过使用异构体检测子宫颈异常细胞的方法
公开(公告)号：US20110124005A1
公开(公告)日：2011-05-26
申请号：US13054755
申请日：2009-07-27
申请人： Masakatsu Morita , Akinori Kawai , Yukio Tsujino
发明人： Masakatsu Morita , Akinori Kawai , Yukio Tsujino
IPC分类号： C12Q1/68 , C07D417/06 , C07D413/06 , C12Q1/16
CPC分类号： G01N33/52 , G01N33/57411 , G01N33/57442 , G01N2800/364
摘要： Disclosed is a reagent for detecting an abnormal cell in the cervix of uterus, which can detect an abnormal cell contained in a biological sample that contains cells collected from the cervix of uterus. The reagent comprises a dye represented by general formula (I).
摘要翻译：公开了一种用于检测子宫颈异常细胞的试剂，其可检测含有从子宫颈收集的细胞的生物样品中含有的异常细胞。该试剂包含由通式（I）表示的染料。

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