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    • 1. 发明申请
    • UP-CONCENTRATION OF ORGANIZ MICROOBJECTS FOR MICROSCOPIC IMAGING
    • 用于微观成像的组织微结构的上调
    • US20120034623A1
    • 2012-02-09
    • US13263997
    • 2010-04-14
    • Bas HulskenStein KuiperSjoerd StallingaBo Joakim IsakssonMark W.G. PonjeeBart E.G.J. Van MeerbergenZeynep S. Unay
    • Bas HulskenStein KuiperSjoerd StallingaBo Joakim IsakssonMark W.G. PonjeeBart E.G.J. Van MeerbergenZeynep S. Unay
    • G01N33/566C12M1/34C12Q1/02
    • G01N1/4077G01N1/4005G01N15/0625G01N15/0631
    • A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.
    • 提出了一种分析含有机微物体样品液的方法。 该方法包括以下步骤:通过在总时间T1中去除来自上升浓度样品微物体的样品流体的体积V1来上升(S1)微观对象; 将微量物体浸入(S2)到转移流体中,或将微量物体留在样品流体的剩余部分中,然后将样品流体的剩余部分提供转移流体; 在总时间T3中过滤(S3)通过过滤器传送流体的体积V3,从而将微物体累积在过滤器上; 以及生成(S4)累积在所述过滤器上的所述微物体的图像; 其特征在于,上述浓缩步骤(S1)的吞吐量V1 / T1大于滤波步骤的吞吐量V1 / T1(S3)。 过滤器可以是第二过滤器,并且上集中(S1)的步骤可以包括:通过第一过滤器过滤样品流体,从而将微物体累积在第一过滤器上。 还公开了一种用于分析含有机微物体的样品流体的装置或系统。