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1. 发明授权

US5208332A Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme 失效
标题翻译：光学探针为细胞色素P-450胆固醇侧链切割酶
公开(公告)号：US5208332A
公开(公告)日：1993-05-04
申请号：US811217
申请日：1991-12-20
申请人： Babetta L. Marrone , Daniel J. Simpson , Clifford J. Unkefer , Thomas W. Whaley
发明人： Babetta L. Marrone , Daniel J. Simpson , Clifford J. Unkefer , Thomas W. Whaley
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60
摘要： An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-450.sub.scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondrial membrane, P-450.sub.scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The fluorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the side-chain cleavage reaction during steroidogenesis.
摘要翻译：光学探针能够通过吸收光谱法或灵敏荧光法研究酶活性。特别地，探针提供了监测细胞色素P-450scc酶的活性的能力，其是用于类固醇生物合成的限速酶。位于内线粒体膜上，P-450scc通过胆固醇侧链的顺序氧化催化胆固醇转化为孕烯醇酮和异己醛。荧光探针包括通过连接基团与发色团连接的胆固醇样类固醇。选择发色团在与类固醇底物连接时具有很小的光学响应，并且当从底物和连接基团切割时具有增强的光学响应。因此，可以通过在类固醇发生过程中的侧链切割反应产生可以被光学检测的荧光阴离子。

2. 发明授权

US5110725A Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme 失效
标题翻译：光学探针为细胞色素P-450胆固醇侧链切割酶
公开(公告)号：US5110725A
公开(公告)日：1992-05-05
申请号：US680978
申请日：1991-04-05
申请人： Babetta L. Marrone , Daniel J. Simpson , Clifford J. Unkefer , Thomas W. Whaley
发明人： Babetta L. Marrone , Daniel J. Simpson , Clifford J. Unkefer , Thomas W. Whaley
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60
摘要： An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-450.sub.scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondrial membrane, P-450.sub.scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The fluorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the side-chain cleavage reaction during steroidogenesis.
摘要翻译：光学探针能够通过吸收光谱法或灵敏荧光法研究酶活性。特别地，探针提供了监测细胞色素P-450scc酶的活性的能力，其是用于类固醇生物合成的限速酶。位于内线粒体膜上，P-450scc通过胆固醇侧链的顺序氧化催化胆固醇转化为孕烯醇酮和异己醛。荧光探针包括通过连接基团与发色团连接的胆固醇样类固醇。选择发色团在与类固醇底物连接时具有很小的光学响应，并且当从底物和连接基团切割时具有增强的光学响应。因此，可以通过在类固醇发生过程中的侧链切割反应产生可以被光学检测的荧光阴离子。

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