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1. 发明授权

US08927231B2 Separatome-based protein expression and purification platform 有权
标题翻译：基于分离蛋白的蛋白质表达和纯化平台
公开(公告)号：US08927231B2
公开(公告)日：2015-01-06
申请号：US14056747
申请日：2013-10-17
申请人： Board of Trustees of the University of Arkansas , University of Pittsburgh—Of The Commonwealth System of Higher Education
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/06 , C12P21/00 , C12P21/02 , C12N1/20 , C12N15/70 , C12N15/74 , C12N15/79
CPC分类号： C12P21/00 , B01D15/203 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D15/424 , C12N1/20 , C12N9/00 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/02
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.
摘要翻译：提供了一种基于分离体的重组肽，多肽和蛋白质表达和纯化平台，其基于宿主细胞基因组肽，多肽和蛋白质的结合性质的并置，其具有相应基因在宿主细胞染色体上的特征和位置，例如大肠杆菌，酵母，枯草芽孢杆菌或其他原核生物，昆虫细胞，哺乳动物细胞等。该平台定量描述和鉴定某些基因产物的优先缺失，修饰或抑制以提高色谱分离效率，定义为柱容量增加，色谱柱选择性增加，或两者都增加，重点在于前者。此外，该平台提供了一种计算机化知识工具，给定分离数据和靶重组肽，多肽或蛋白质，直观地提出了导致有效产品纯化的策略。基于分离蛋白的蛋白质表达和纯化平台是一种有效的生物分离系统，其交联宿主细胞表达系统和色谱。

2. 发明授权

US10066232B2 E. coli separatome-based protein expression and purification platform 有权
公开(公告)号：US10066232B2
公开(公告)日：2018-09-04
申请号：US15791803
申请日：2017-10-24
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/06 , C12N9/00 , C12N15/70 , C12P21/02 , C12P21/00 , C12P21/04 , C12N1/21
摘要： Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

3. 发明授权

US10030257B2 Separatome-based protein expression and purification platform 有权
公开(公告)号：US10030257B2
公开(公告)日：2018-07-24
申请号：US15428220
申请日：2017-02-09
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/00 , C12N1/20 , C12N9/00 , B01D15/38 , B01D15/42 , B01D15/20 , B01D15/36 , C12N5/07 , C12N15/09
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

4. 发明申请

US20180073030A1 E. coli Separatome-Based Protein Expression And Purification Platform 审中-公开
公开(公告)号：US20180073030A1
公开(公告)日：2018-03-15
申请号：US15791803
申请日：2017-10-24
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. Brune , Robert R. Beitle, JR. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12N15/70 , C12N9/00 , C12P21/00 , C12P21/02
CPC分类号： C12N15/70 , C12N9/00 , C12P21/00 , C12P21/02
摘要： Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

5. 发明申请

US20170218422A1 SEPARATOME-BASED PROTEIN EXPRESSION AND PURIFICATION PLATFORM 审中-公开
公开(公告)号：US20170218422A1
公开(公告)日：2017-08-03
申请号：US15428220
申请日：2017-02-09
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH--OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. BRUNE , Robert R. Beitle, JR. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/00 , C12N9/00 , B01D15/36 , B01D15/42 , B01D15/20 , C12N1/20 , B01D15/38
CPC分类号： C12P21/00 , B01D15/203 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D15/424 , C12N1/20 , C12N9/00 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/02
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

6. 发明申请

US20190055570A1 E. coli Separatome-Based Protein Expression And Purification Platform 审中-公开
公开(公告)号：US20190055570A1
公开(公告)日：2019-02-21
申请号：US16113840
申请日：2018-08-27
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12N15/70 , C12N9/00 , C12P21/02 , C12P21/00
摘要： Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

7. 发明授权

US09822371B2 E. coli separatome-based protein expression and purification platform 有权
公开(公告)号：US09822371B2
公开(公告)日：2017-11-21
申请号：US15022852
申请日：2014-09-17
申请人： Board Of Trustees Of The University of Arkansas , University Of Pittsburgh—Of The Commonwealth System Of Higher Education
发明人： Ellen M. Brune , Robert R. Beitle , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/06 , C12N9/00 , C12N15/70 , C12P21/02 , C12P21/00 , C12P21/04 , C12N1/21
CPC分类号： C12N15/70 , C12N9/00 , C12P21/00 , C12P21/02
摘要： Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data, and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

8. 发明授权

US09816068B2 Separatome-based protein expression and purification platform 有权
公开(公告)号：US09816068B2
公开(公告)日：2017-11-14
申请号：US14521845
申请日：2014-10-23
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12N1/20 , C12P21/02 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/00 , C12N1/14 , C12N5/07 , C12N15/09 , C12P21/06
CPC分类号： C12P21/00 , B01D15/203 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D15/424 , C12N1/20 , C12N9/00 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/02
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

9. 发明申请

US20180327802A1 SEPARATOME-BASED PROTEIN EXPRESSION AND PURIFICATION PLATFORM 审中-公开
公开(公告)号：US20180327802A1
公开(公告)日：2018-11-15
申请号：US16040000
申请日：2018-07-19
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCTION
发明人： Ellen M. Brune , Robert R. Beitle, Jr. , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12P21/00 , C12N15/79 , C12N15/74 , C12N15/70 , C12P21/02 , C12N9/00 , C12N1/20 , B01D15/38 , B01D15/36 , B01D15/20 , B01D15/42
CPC分类号： C12P21/00 , B01D15/203 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D15/424 , C12N1/20 , C12N9/00 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/02
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.

10. 发明申请

US20150139944A1 SEPARATONE-BASED PROTEIN EXPRESSION AND PURIFICATION PLATFORM 有权
标题翻译：基于SEPARATONE的蛋白质表达和纯化平台
公开(公告)号：US20150139944A1
公开(公告)日：2015-05-21
申请号：US14521845
申请日：2014-10-23
申请人： BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
发明人： Ellen M. Brune , Robert R. Beitle , Mohammad M. Ataai , Patrick R. Bartlow , Ralph L. Henry
IPC分类号： C12N1/20 , C12P21/00
CPC分类号： C12P21/00 , B01D15/203 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D15/424 , C12N1/20 , C12N9/00 , C12N15/70 , C12N15/74 , C12N15/79 , C12P21/02
摘要： Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.
摘要翻译：提供了一种基于分离体的重组肽，多肽和蛋白质表达和纯化平台，其基于宿主细胞基因组肽，多肽和蛋白质的结合性质的并置，其具有相应基因在宿主细胞染色体上的特征和位置，例如大肠杆菌，酵母，枯草芽孢杆菌或其他原核生物，昆虫细胞，哺乳动物细胞等。该平台定量描述和鉴定某些基因产物的优先缺失，修饰或抑制以提高色谱分离效率，定义为柱容量增加，色谱柱选择性增加，或两者都增加，重点在于前者。此外，该平台提供了一种计算机化知识工具，给定分离数据和靶重组肽，多肽或蛋白质，直观地提出了导致有效产品纯化的策略。基于分离蛋白的蛋白质表达和纯化平台是一种有效的生物分离系统，其交联宿主细胞表达系统和色谱。

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