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1. 发明公开

US20230293615A1 MATERIALS AND METHODS FOR PUFA PRODUCTION, AND PUFA-CONTAINING COMPOSITIONS 审中-公开
公开(公告)号：US20230293615A1
公开(公告)日：2023-09-21
申请号：US18162300
申请日：2023-01-31
申请人： BASF PLANT SCIENCE COMPANY GMBH , Bioriginal Food & Science Corporation
发明人： Toralf Senger , Laurent Marty , Irene Kunze , Heiko Haertel , Steven Craig Bremmer , Steven D. Breazeale , Joerg Bauer , Patricia Vrinten , Sten Stymne , Jenny Lindberg Yilmaz , John A. McElver , Dietrich Rein , Carl Andre
IPC分类号： A61K36/31 , C12Q1/6895
CPC分类号： A61K36/31 , C12Q1/6895 , C12N15/8247
摘要： T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

2. 发明授权

US11613761B1 Materials and methods for PUFA production, and PUFA-containing compositions 有权
公开(公告)号：US11613761B1
公开(公告)日：2023-03-28
申请号：US15526550
申请日：2015-11-13
申请人： BASF PLANT SCIENCE COMPANY GMBH , BIORIGINAL FOOD & SCIENCE CORPORATION
发明人： Toralf Senger , Laurent Marty , Irene Kunze , Heiko Haertel , Steven Craig Bremmer , Steven D. Breazeale , Joerg Bauer , Patricia Vrinten , Sten Stymne , Jenny Lindberg Yilmaz , John A. McElver , Dietrich Rein , Carl Andre
IPC分类号： C12N15/82 , C12N9/02
摘要： T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

3. 发明授权

US11046937B2 Methods for conversion of the substrate specificity of desaturases 有权
公开(公告)号：US11046937B2
公开(公告)日：2021-06-29
申请号：US16414150
申请日：2019-05-16
申请人： BASF PLANT SCIENCE COMPANY GMBH , Bioriginal Food & Science Corp.
发明人： Toralf Senger , Patricia Vrinten , Ze Long Lim
IPC分类号： C12N9/02 , C07K14/46 , C12P7/64
摘要： The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

4. 发明公开

US20230183728A1 IMPROVED METHOD FOR THE PRODUCTION OF HIGH LEVELS OF PUFA IN PLANTS 审中-公开
公开(公告)号：US20230183728A1
公开(公告)日：2023-06-15
申请号：US17273858
申请日：2019-09-06
申请人： BASF PLANT SCIENCE COMPANY GMBH
发明人： Toralf Senger , Hui Yang , Carl Andre , Patricia Vrinten
IPC分类号： C12N15/82 , C12N9/12 , C07K16/40 , A23D9/00 , A23L33/115
CPC分类号： C12N15/8247 , A23D9/00 , A23L33/115 , C07K16/40 , C12N9/1288 , C12N15/8218 , C12Y207/08002 , A23V2002/00
摘要： The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

5. 发明申请

US20190270972A1 METHODS FOR CONVERSION OF THE SUBSTRATE SPECIFICITY OF DESATURASES 审中-公开
公开(公告)号：US20190270972A1
公开(公告)日：2019-09-05
申请号：US16414150
申请日：2019-05-16
申请人： BASF PLANT SCIENCE COMPANY GMBH , Bioriginal Food & Science Corp.
发明人： Toralf Senger , Patricia Vrinten , Ze Long Lim
IPC分类号： C12N9/02 , C12P7/64 , C07K14/46
摘要： The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

6. 发明授权

US10329541B2 Methods for conversion of the substrate specificity of desaturases 有权
公开(公告)号：US10329541B2
公开(公告)日：2019-06-25
申请号：US15105080
申请日：2014-12-17
申请人： BASF PLANT SCIENCE COMPANY GMBH
发明人： Toralf Senger , Patricia Vrinten , Ze Long Lim
IPC分类号： C12N9/02 , C07K14/46 , C12P7/64
摘要： The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

7. 发明申请

US20220025391A1 IMPROVED METHOD FOR THE PRODUCTION OF HIGH LEVELS OF PUFA IN PLANTS 有权
公开(公告)号：US20220025391A1
公开(公告)日：2022-01-27
申请号：US17273799
申请日：2019-09-06
申请人： BASF PLANT SCIENCE COMPANY GMBH
发明人： Toralf Senger , Hui Yang , Patricia Vrinten , Carl Andre
IPC分类号： C12N15/82 , C12N9/12 , A23D9/013 , A23D9/02 , A23L33/115 , A23L33/12 , A23K20/158
摘要： The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

8. 发明申请

US20160319249A1 METHODS FOR CONVERSION OF THE SUBSTRATE SPECIFICITY OF DESATURASES 审中-公开
公开(公告)号：US20160319249A1
公开(公告)日：2016-11-03
申请号：US15105080
申请日：2014-12-17
申请人： BASF PLANT SCIENCE COMPANY GMBH
发明人： Toralf Senger , Patricia Vrinten , Ze Long Lim
IPC分类号： C12N9/02 , C12P7/64
CPC分类号： C12N9/0071 , C07K14/461 , C12N9/0083 , C12P7/6427 , C12P7/6472 , C12Y114/19
摘要： The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity.

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