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    • 10. 发明授权
    • Centimeter-scale, integrated diagnostics incubator for biological culturing
    • 用于生物培养的厘米级综合诊断培养箱
    • US07855070B2
    • 2010-12-21
    • US11483126
    • 2006-07-07
    • Jelena VukasinovicAri Glezer
    • Jelena VukasinovicAri Glezer
    • C12M1/00C12M3/00
    • C12M41/12B33Y80/00C12M23/16C12M23/22C12M29/10C12M35/02
    • Disclosed are portable, disposable, centimeter-scale, integrated diagnostics incubators for use in biological culturing. An exemplary incubator comprises an optically accessible enclosure having a plurality of fluidic ports. A heating element is disposed within the enclosure that is coupled to an external heater controller. An autoclavable microfluidic perfusion chamber is disposed within the enclosure that comprises a cell culture life support chamber, an inlet port disposed in the perfusion chamber, a collection chamber in communication with the culture chamber, an outlet port coupled to the collection pool, and a perfusing substrate. An optically transparent, gas permeable membrane is attachable to the top of the perfusion chamber. The incubators have optical accessibility, forced flow fluidic control, temperature control, are portable and modular, and are inexpensively manufactured. The incubators permit in-the-field drug testing and culturing of biological tissues.
    • 公开了用于生物培养的便携式,一次性,厘米级的综合诊断培养箱。 示例性的培养箱包括具有多个流体端口的可光学访问的外壳。 加热元件设置在外壳内,其耦合到外部加热器控制器。 可灭菌的微流体灌注室设置在外壳内,其包括细胞培养寿命支持室,设置在灌注室中的入口端口,与培养室连通的收集室,连接到收集池的出口端口和灌注 基质。 透光透气膜可连接到灌注腔的顶部。 孵化器具有光学可及性,强制流动流体控制,温度控制,便携式和模块化,并且经济实惠。 孵化器允许现场药物检测和培养生物组织。