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1. 发明授权

US5667980A Method for assaying for a substance that affects an SH2 phosphorylated ligand regulatory system 失效
标题翻译：测定影响SH2磷酸化配体调节系统的物质的方法
公开(公告)号：US5667980A
公开(公告)日：1997-09-16
申请号：US221699
申请日：1994-03-28
申请人： Anthony Pawson , Michael Reedijk
发明人： Anthony Pawson , Michael Reedijk
IPC分类号： G01N33/566 , G01N33/573 , G01N33/74 , C12Q1/48 , G01N33/53 , G01N33/68
CPC分类号： G01N33/573 , G01N33/566 , G01N33/74 , G01N2333/71 , G01N2333/9121 , G01N2500/20
摘要： A method for assaying a medium for the presence of a substance that affects an SH2-phosphorylated ligand regulatory system. The method employs an SH2-like domain or a subdomain thereof and a phosphorylated ligand. The phosphophorylated ligand is capable of interacting with the SH2-like domain or a subdomain thereof to form an SH2-phosphorylated ligand complex. The SH2-like domain or subdomain and/or the phosphorylated ligand are present in a known concentration. The SH2-like domain or a subdomain thereof and the phosphorylated ligand are incubated with a substance which is suspected of affecting an SH2-phosphorylated ligand regulatory system. The method is carried out under conditions which permit the formation of the SH2-phosphorylated ligand complex. SH2-phosphorylated ligand complex, free SH2-like domain or subdomains thereof, or non-complexed phosphorylated ligand are assayed. The invention also relates to an isolated SH2-phosphorylated ligand complex; a method of using an isolated SH2-like domain or a subdomain thereof to screen for phosphorylated ligands which are active in an SH2-phosphorylated ligand regulatory system; a method of using an isolated SH2-like domain or a subdomain thereof to regulate the interaction of a signalling protein with a related phosphorylated ligand; and a pharmaceutical composition comprising an isolated SH2-like domain or a subdomain thereof for use as an agohist or antagonist of the interaction of a signalling protein with a related phosphorylated ligand.
摘要翻译：用于测定培养基中存在影响SH2磷酸化配体调节系统的物质的方法。该方法采用SH2样结构域或其亚结构域和磷酸化配体。磷酰化配体能够与SH2样结构域或其亚结构域相互作用形成SH2-磷酸化的配体复合物。 SH2样结构域或亚结构域和/或磷酸化配体以已知浓度存在。将SH2样结构域或其亚结构域和磷酸化配体与怀疑影响SH2-磷酸化配体调节系统的物质一起温育。该方法在允许形成SH2-磷酸化配体络合物的条件下进行。测定SH2-磷酸化配体复合物，游离SH2样结构域或其子结构域，或非络合磷酸化配体。本发明还涉及分离的SH2-磷酸化配体复合物; 使用分离的SH2样结构域或其亚结构域筛选在SH2磷酸化配体调节系统中有活性的磷酸化配体的方法; 使用分离的SH2样结构域或其亚结构域来调节信号蛋白与相关磷酸化配体的相互作用的方法; 以及包含分离的SH2样结构域或其子结构域的药物组合物，用作信号蛋白与相关磷酸化配体的相互作用的前组或拮抗剂。

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