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1. 发明授权

US07932027B2 Method for determining the methylation pattern of a polynucleic acid 有权
标题翻译：确定多核酸甲基化模式的方法
公开(公告)号：US07932027B2
公开(公告)日：2011-04-26
申请号：US11355417
申请日：2006-02-16
申请人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
发明人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2531/113 , C12Q2525/191 , C12Q2521/331
摘要： Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.
摘要翻译：具体方面涉及确定多核酸的甲基化模式的方法，其包括：a）制备包含所述多核酸的片段的混合物的溶液; b）将片段与可用检测方法检测的物质偶联; c）使包含b）的片段的溶液与分别具有多个不同的固定化寡核苷酸的DNA微阵列接触，所述多个不同的固定化寡核苷酸分别包含至少一个甲基化位点，分别在其上指定了不同的位置，在提供相关的固定化寡核苷酸的片段杂交的条件下进行接触在限定的严格性下，并且其中所述固定的寡核苷酸具有小于200个碱基的长度; d）任选地执行洗涤步骤; 以及e）使用所述物理检测方法检测将所述固定化的核酸与所述溶液片段杂交并且/或所述溶液片段未杂交的固定化核酸。

2. 发明申请

US20060204988A1 Method for determining the methylation pattern of a polynucleic acid 有权
标题翻译：确定多核酸甲基化模式的方法
公开(公告)号：US20060204988A1
公开(公告)日：2006-09-14
申请号：US11355417
申请日：2006-02-16
申请人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
发明人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q2531/113 , C12Q2525/191 , C12Q2521/331
摘要： Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacing under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.
摘要翻译：具体方面涉及确定多核酸的甲基化模式的方法，其包括：a）制备包含所述多核酸的片段的混合物的溶液; b）将片段与可用检测方法检测的物质偶联; c）使包含b）的片段的溶液与分别具有多个不同的固定化寡核苷酸的DNA微阵列接触，每个寡核苷酸分别包含至少一个甲基化位点，分别在其上指定不同的位置，在提供相关的固定化寡核苷酸的片段杂交的条件下在限定的严格性下，并且其中所述固定的寡核苷酸具有小于200个碱基的长度; d）任选地执行洗涤步骤; 以及e）使用所述物理检测方法检测将所述固定化的核酸与所述溶液片段杂交并且/或所述溶液片段未杂交的固定化核酸。

3. 发明申请

US20120129729A1 Method for Determining the Methylation Pattern of a Polynucleic Acid 审中-公开
标题翻译：确定多核酸甲基化模式的方法
公开(公告)号：US20120129729A1
公开(公告)日：2012-05-24
申请号：US13079721
申请日：2011-04-04
申请人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
发明人： Anne Fassbender , Ralf Lesche , Juergen Distler , Christian Piepenbrock , Tamas Rujan , Kurt Berlin , Thomas Koenig
IPC分类号： C40B40/06
CPC分类号： C12Q1/6827 , C12Q2531/113 , C12Q2525/191 , C12Q2521/331
摘要： Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.
摘要翻译：具体方面涉及确定多核酸的甲基化模式的方法，其包括：a）制备包含所述多核酸的片段的混合物的溶液; b）将片段与可用检测方法检测的物质偶联; c）使包含b）的片段的溶液与分别具有多个不同的固定化寡核苷酸的DNA微阵列接触，所述多个不同的固定化寡核苷酸分别包含至少一个甲基化位点，分别在其上指定了不同的位置，在提供相关的固定化寡核苷酸的片段杂交的条件下进行接触在限定的严格性下，并且其中所述固定的寡核苷酸具有小于200个碱基的长度; d）可选地执行洗涤步骤; 以及e）使用所述物理检测方法检测将所述固定化的核酸与所述溶液片段杂交并且/或所述溶液片段未杂交的固定化核酸。

4. 发明授权

US09695478B2 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
公开(公告)号：US09695478B2
公开(公告)日：2017-07-04
申请号：US12779856
申请日：2010-05-13
申请人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

5. 发明申请

US20060286576A1 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US20060286576A1
公开(公告)日：2006-12-21
申请号：US11405322
申请日：2006-04-17
申请人： Cathy Lofton-Day , Andrew Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Cathy Lofton-Day , Andrew Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和鉴别肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

6. 发明授权

US07749702B2 Methods and nucleic acids for the analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US07749702B2
公开(公告)日：2010-07-06
申请号：US11405322
申请日：2006-04-17
申请人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine E. Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , C12Q1/44 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和鉴别肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

7. 发明申请

US20100184027A1 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：US20100184027A1
公开(公告)日：2010-07-22
申请号：US12373709
申请日：2007-07-13
申请人： Catherine Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
发明人： Catherine Lofton-Day , Andrew Z. Sledziewski , Ralf Lesche , Matthias Schuster , Juergen Distler , Reimo Tetzner , Thomas Hildmann , Fabian Model , Xiaoling Song
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和区分增殖性疾病之间或之中的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

8. 发明申请

US20090075260A1 Methods and Nucleic Acids For Analysis of Cellular Proliferative Disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US20090075260A1
公开(公告)日：2009-03-19
申请号：US11911617
申请日：2006-04-17
申请人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
发明人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之中的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

9. 发明授权

US08900829B2 Methods and nucleic acids for analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US08900829B2
公开(公告)日：2014-12-02
申请号：US13096932
申请日：2011-04-28
申请人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
发明人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之中的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

10. 发明申请

US20110244458A1 Methods and Nucleic Acids for Analyses of Cellular Proliferative Disorders 有权
标题翻译：方法和核酸分析细胞增殖性疾病
公开(公告)号：US20110244458A1
公开(公告)日：2011-10-06
申请号：US13096932
申请日：2011-04-28
申请人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
发明人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
IPC分类号： C12Q1/68 , G01N33/50 , C07H21/00
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之中的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

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