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    • 3. 发明授权
    • Enzymatic oxidation of alcohols to aldehydes in a continuous reaction
system
    • 醇在连续反应体系中的酶催化氧化成醛
    • US5783429A
    • 1998-07-21
    • US728085
    • 1996-10-09
    • Alan GabelmanGary A. Luzio
    • Alan GabelmanGary A. Luzio
    • C12P7/24
    • C12P7/24Y10S435/911Y10S435/921
    • A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism such as those selected from the genera of Pichia, Hansenula, Candida and Torulopsis. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted. In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact yeast cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
    • 利用粗细胞甲醇氧化酶和由可接受的生物如由毕赤酵母,汉逊酵母属,假丝酵母​​属和Torulopsis属中选择的生物体产生的过氧化氢酶,公开了将醇类酶连续反应的酶氧化成醛的方法。 该过程保持稳态醛浓度,从而促进酶活性。 特别地,该方法具有串联连续生产含有生长的完整酵母细胞的发酵罐流出物的两个反应器,其浓度为流出物重量的约0.5至约3重量%,通过在甲醇碳源上连续培养细胞, 将来自第一反应器的流出物连续引入第二反应器,向第二反应器中加入约0.2-10%w / w的乙醇进料和氧,保持反应混合物中的稳态醛浓度,残余醇的浓度 通过控制流出物和醇饲料的引入速率约为0.1-9%w / w。
    • 5. 发明授权
    • Enzymatic oxidation of alcohols to aldehydes in a continuous reaction
system using Candida boidinii
    • 在使用念珠菌的连续反应系统中将醇酶化为醛
    • US5593872A
    • 1997-01-14
    • US524911
    • 1995-09-08
    • Alan GabelmanGary A. Luzio
    • Alan GabelmanGary A. Luzio
    • C12P7/24
    • C12P7/24Y10S435/921
    • A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted and side reactions are eliminated.In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact Candida boidinii cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde and alcohol concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
    • 使用粗细胞甲醇氧化酶和由可接受的生物体产生的过氧化氢酶,公开了连续反应系列中将醇酶氧化成醛的方法。 该方法维持稳态醛浓度,从而促进酶活性并消除副反应。 特别地,该方法具有串联连续生产含有生长完整的假丝酵母假丝酵母细胞的发酵罐流出物的两个反应器,其浓度为流出物重量的约0.5至约3%,通过在甲醇碳源上连续培养细胞 将来自第一反应器的流出物连续引入第二反应器,向第二反应器中加入约0.2-10%w / w的醇进料和氧气,保持反应混合物中的稳态醛和醇浓度,浓度 的残留酒精为约0.1-9%w / w,通过控制流出物和醇进料的引入速率。