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    • 10. 发明授权
    • High performance microcapillary gel electrophoresis
    • 高性能微毛细管凝胶电泳
    • US4865706A
    • 1989-09-12
    • US921311
    • 1986-10-21
    • Barry L. KargerAharon Cohen
    • Barry L. KargerAharon Cohen
    • G01N27/447
    • G01N27/44721G01N27/44704G01N27/44747Y10T29/49885
    • A microcapillary column for high performance electrophoresis includes a fused silica microcapillary, a gel of crosslinked polyacrylamide polymerized in the tube, and a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The gel-containing microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates within separation times of less than thirty minutes, and permits trace level determinations of molecular weights.
    • 用于高性能电泳的微毛细管柱包括熔融二氧化硅微毛细管,在管中聚合的交联聚丙烯酰胺的凝胶和共价键合到微毛细管壁的内表面和聚合物凝胶的薄层连接材料。 通过首先将合适的双功能试剂共价结合到微毛细管壁的内表面,然后使单体,交联剂和聚合催化剂的混合物在微毛细孔的孔中反应以形成聚合物,制备含凝胶的微毛细管 通过双功能试剂共价结合到微毛细管壁上的基质。 在电泳过程中,含凝胶微毛细管提供超过100,000理论塔板的峰值效率,分离时间少于30分钟,并允许痕量水平测定分子量。