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1. 发明申请

US20080145882A1 Mutant hydrolase proteins with enhanced kinetics and functional expression 有权
标题翻译：具有增强的动力学和功能表达的突变水解酶蛋白
公开(公告)号：US20080145882A1
公开(公告)日：2008-06-19
申请号：US11978950
申请日：2007-10-30
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
IPC分类号： C12Q1/34 , C12N9/78 , C12N11/02 , C12N11/12 , C07H21/04 , C12N1/20
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
摘要翻译：本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。

2. 发明申请

US20130302880A1 POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION 有权
公开(公告)号：US20130302880A1
公开(公告)日：2013-11-14
申请号：US13863924
申请日：2013-04-16
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
IPC分类号： C12N9/14
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

3. 发明授权

US08420367B2 Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression 有权
标题翻译：编码突变体水解酶蛋白的多核苷酸具有增强的动力学和功能表达
公开(公告)号：US08420367B2
公开(公告)日：2013-04-16
申请号：US11978950
申请日：2007-10-30
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
IPC分类号： C12N15/00 , C12N1/21 , C12N5/10 , C12P21/00 , C07H21/00 , C12N9/14
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
摘要翻译：本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。

4. 发明申请

US20090305280A1 Luciferase biosensors for camp 有权
标题翻译：萤光素酶生物传感器
公开(公告)号：US20090305280A1
公开(公告)日：2009-12-10
申请号：US12454643
申请日：2009-05-19
申请人： Brock Binkowski , Lance P. Encell , Monika G. Wood , Keith V. Wood , Kris Zimmerman , Paul Otto , Gediminas Vidugiris , Pete Stecha
发明人： Brock Binkowski , Lance P. Encell , Monika G. Wood , Keith V. Wood , Kris Zimmerman , Paul Otto , Gediminas Vidugiris , Pete Stecha
IPC分类号： C12Q1/68 , C07H21/00 , C12N15/63 , C12N9/12 , C12Q1/66
CPC分类号： C12Q1/66 , C07K2319/20 , C07K2319/61 , C12N9/0004 , C12N9/0069 , C12Q1/008 , C12Q1/6876 , C12Y113/12 , C12Y114/00 , G01N33/5735 , G01N33/74 , G01N2333/726 , G01N2333/90241
摘要： A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.
摘要翻译：提供了一种修饰的荧光素酶蛋白，其是包括cAMP的分子的传感器。修饰的荧光素酶蛋白包括一个或多个异源氨基酸序列，其中至少一个直接或间接与cAMP相互作用。

5. 发明授权

US09045730B2 Luciferase biosensors for cAMP 有权
标题翻译： cAMP的荧光素酶生物传感器
公开(公告)号：US09045730B2
公开(公告)日：2015-06-02
申请号：US12454643
申请日：2009-05-19
申请人： Brock Binkowski , Lance P. Encell , Monika G. Wood , Keith V. Wood , Kris Zimmerman , Paul Otto , Gediminas Vidugiris , Pete Stecha
发明人： Brock Binkowski , Lance P. Encell , Monika G. Wood , Keith V. Wood , Kris Zimmerman , Paul Otto , Gediminas Vidugiris , Pete Stecha
IPC分类号： C12N9/02 , C12N15/53 , C12Q1/66 , C12Q1/00 , G01N33/573
CPC分类号： C12Q1/66 , C07K2319/20 , C07K2319/61 , C12N9/0004 , C12N9/0069 , C12Q1/008 , C12Q1/6876 , C12Y113/12 , C12Y114/00 , G01N33/5735 , G01N33/74 , G01N2333/726 , G01N2333/90241
摘要： A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.
摘要翻译：提供了一种修饰的荧光素酶蛋白，其是包括cAMP的分子的传感器。修饰的荧光素酶蛋白包括一个或多个异源氨基酸序列，其中至少一个直接或间接与cAMP相互作用。

6. 发明授权

US08557970B2 Synthetic Oplophorus luciferases with enhanced light output 有权
标题翻译：合成的Oplophorus萤光素酶具有增强的光输出
公开(公告)号：US08557970B2
公开(公告)日：2013-10-15
申请号：US12773002
申请日：2010-05-03
申请人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
发明人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
IPC分类号： C12N15/53 , C12N15/63 , C12N15/70 , C12N15/79 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译：编码经修饰的萤光素酶多肽的多核苷酸。修饰的荧光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus荧光素酶中的氨基酸的位置包括至少一个氨基酸取代。修饰的荧光素酶多肽相对于野生型Oplophorus萤光素酶具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。

7. 发明申请

US20100281552A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
标题翻译：具有增强光输出功能的合成眼科植入物
公开(公告)号：US20100281552A1
公开(公告)日：2010-11-04
申请号：US12773002
申请日：2010-05-03
申请人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
发明人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
IPC分类号： A01K67/027 , C07H21/00 , C12N15/63 , C12N5/10 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译：编码经修饰的萤光素酶多肽的多核苷酸。修饰的荧光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus荧光素酶中的氨基酸的位置包括至少一个氨基酸取代。修饰的荧光素酶多肽相对于野生型Oplophorus萤光素酶具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。

8. 发明授权

US08735559B2 Mutant protease biosensors with enhanced detection characteristics 有权
标题翻译：具有增强检测特性的突变蛋白酶生物传感器
公开(公告)号：US08735559B2
公开(公告)日：2014-05-27
申请号：US13105648
申请日：2011-05-11
申请人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
发明人： Brock Binkowski , Braeden Butler , Lance P. Encell , Frank Fan , Brad Hook , Paul Otto , Gediminas Vidugiris , Susan Wigdal , Kristopher Zimmerman
IPC分类号： C12N15/62 , C12N15/63 , C12N15/54 , C12N15/74 , C12N15/79
CPC分类号： A61K49/0045 , A01K67/0275 , A61K49/0013 , C12N9/0069 , C12Q1/37 , C12Q1/66 , C12Y113/12007
摘要： A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
摘要翻译：编码包含修饰的环状置换的热稳定性荧光素酶的生物传感器多肽的多核苷酸和将热稳定性荧光素酶的C末端部分连接到热稳定性萤光素酶的N末端部分的接头。相对于亲本循环置换的热稳定性荧光素酶，修饰的环状置换的热稳定性荧光素酶被修饰。接头包含能够与细胞中的靶分子相互作用的传感器区域。经修饰的环状置换的热稳定荧光素酶在目标分子存在下相对于亲本循环置换的热稳定性荧光素酶在生物传感器与靶分子相互作用后具有增强的响应。或者，修饰的环状置换的热稳定荧光素酶在生物传感器与目标分子相互作用后相对于在不存在目标分子的情况下相对于经修饰的环状置换的热稳定性荧光素酶具有增强的响应。

9. 发明授权

US6077664A Thermophilic DNA polymerases from Thermotoga neapolitana 失效
标题翻译：来自Thermotoga neapolitana的嗜热DNA聚合酶
公开(公告)号：US6077664A
公开(公告)日：2000-06-20
申请号：US656664
申请日：1996-05-31
申请人： Michael R. Slater , Fen Huang , James R. Hartnett , Elena Bolchakova , Douglas R. Storts , Paul Otto , Katharine M. Miller , Alexander Novikov , Galina A. Velikodvorskaya
发明人： Michael R. Slater , Fen Huang , James R. Hartnett , Elena Bolchakova , Douglas R. Storts , Paul Otto , Katharine M. Miller , Alexander Novikov , Galina A. Velikodvorskaya
IPC分类号： C12N1/21 , C12N9/12 , C12N15/54 , C12Q1/68 , C07H21/04 , C12N9/00 , C12P19/34
CPC分类号： C12N9/1252 , C12Q1/686 , C12Q1/6869
摘要： The present invention relates to compositions of thermostable DNA polymerases derived from the hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterium known as Thermotoga neapolitana. The present invention provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. neopolitana DNA polymerase. The T. neopolitana DNA polymerases of the present invention are used in combination with other compounds, including but not limited to pyrophosphatase and DNA polymerases from other thermophilic or hyperthermophilic organisms.
摘要翻译：本发明涉及源于超嗜热真细菌的热稳定DNA聚合酶的组合物。特别地，本发明包括来自称为Thermotoga neapolitana的超嗜热真细菌的热稳定DNA聚合酶。本发明提供了利用天然存在和非天然存在形式的新孢子虫DNA聚合酶的方法。本发明的新孢杆菌DNA聚合酶与其它化合物组合使用，包括但不限于焦磷酸酶和来自其他嗜热或超嗜热生物的DNA聚合酶。

10. 发明授权

US5579454A Three dimensional graphics processing with pre-sorting of surface portions 失效
标题翻译：三维图形处理，预处理表面部分
公开(公告)号：US5579454A
公开(公告)日：1996-11-26
申请号：US937701
申请日：1992-09-01
申请人： Adam Billyard , Paul Otto , David Lau-Kee
发明人： Adam Billyard , Paul Otto , David Lau-Kee
IPC分类号： G06T15/40
CPC分类号： G06T15/405
摘要： To effect hidden surface removal, polygons making up a three dimensional object are initially ordered using a Topological Sort into, for example, three rendering order lists corresponding to orthogonal axis. When the object is to be represented from a given view direction, the view direction is compared to the axis and the rendering list corresponding to the closest match is selected. Polygons are then rendered in list order, backward facing polygons being omitted. Performing the ordering at the polygon level, in an initial step, saves computation and consequently enables the projection and rendering processes to be speeded up to provide real time interactive three dimensional graphics. The Topological Sorting process is particularly computationally effective.
摘要翻译：为了实现隐藏的表面去除，构成三维对象的多边形最初使用拓扑排序来排序，例如对应于正交轴的三个渲染顺序列表。当从给定的视图方向来表示对象时，将视图方向与轴进行比较，并且选择与最接近的匹配相对应的渲染列表。然后以列表顺序呈现多边形，省略向后的多边形。在初始步骤中，在多边形级别执行排序可以节省计算量，从而使投影和渲染过程能够加快，以提供实时的交互式三维图形。拓扑排序过程在计算上特别有效。

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