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1. 发明申请

US20070169224A1 Novel vector and method of constructing transformant plant using the vector 审中-公开
标题翻译：使用载体构建转化体植物的新型载体和方法
公开(公告)号：US20070169224A1
公开(公告)日：2007-07-19
申请号：US10552146
申请日：2004-04-07
申请人： Akiyoshi Kawaoka , Kazuya Nanto , Hiroyasu Ebinuma
发明人： Akiyoshi Kawaoka , Kazuya Nanto , Hiroyasu Ebinuma
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8216
摘要： Gene introduction to a plant is carried out by using a vector which comprises the following constitutional units A and B: A: a DNA sequence comprising P1, an expression inhibitory sequence and a desired gene which is controlled by P1 and the expression inhibitory sequence; B: a DNA sequence comprising P2, an expression inhibitory gene which is controlled by P2, P3, the expression inhibitory sequence, and a gene of a removal reaction-catalyzing enzyme which is controlled by P3 and the expression inhibitory sequence, wherein the DNA sequence is removed by expression of the gene of a removal reaction-catalyzing enzyme, wherein P1 is a promoter which shows its activity in at least a callus and a plant tissue where a desired gene should be expressed, P2 is a promoter which shows its activity in at least a callus, and P3 is a promoter which shows its activity in at least a plant tissue where P1 and P2 do not show their activities.
摘要翻译：通过使用包含以下结构单元A和B的载体进行植物的基因导入：A：包含P1，表达抑制性序列和由P1控制的所需基因的DNA序列和表达抑制性序列; B：包含P2，由P2，P3控制的表达抑制基因，表达抑制性序列和由P3控制的去除反应催化酶的基因和表达抑制性序列的DNA序列，其中所述DNA序列通过去除反应催化酶的基因的表达除去，其中P1是至少在愈伤组织中显示其活性的启动子和其中应该表达所需基因的植物组织，P2是显示其活性的启动子至少一个愈伤组织，P3是在P1和P2不显示其活性的至少一种植物组织中显示其活性的启动子。

2. 发明申请

US20090320164A1 METHOD FOR PRODUCTION OF PLANT CELL HAVING CHROMOSOME LOSS 审中-公开
标题翻译：用于生产染色体损失的植物细胞的方法
公开(公告)号：US20090320164A1
公开(公告)日：2009-12-24
申请号：US11721273
申请日：2005-12-08
申请人： Kazuya Nanto , Hiroyasu Ebinuma
发明人： Kazuya Nanto , Hiroyasu Ebinuma
IPC分类号： A01H5/00 , C12N15/82 , C12N5/10
CPC分类号： C12N15/8213 , C12N15/82
摘要： The present invention provides a simple aneuploid production process, applicable to all species, without producing unexpected damage to chromosomes other than a chromosome which is to be disappeared. A vector comprising two site-specific recognition sequences oriented in the opposite direction, or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence, and a recombinase gene is introduced into a plant cells or a vector comprising two site-specific recognition sequences oriented in the opposite directions or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence is introduced into a plant cell; a recombinase is allowed to act transiently in the cell during at least growth of the cell; and the cell is cultured and grown; and a cell in which a predetermined chromosome is disappeared is selected.
摘要翻译：本发明提供了一种适用于所有物种的简单的非整倍体生产方法，而不会对将要消失的染色体以外的染色体产生意外的损害。将包含相反方向取向的两个位点特异性识别序列或包含点对称核苷酸序列的一个位点特异性重组酶识别序列和重组酶基因的载体导入植物细胞或包含两个位点特异性识别的载体将指向相反方向的序列或包含点对称核苷酸序列的一个位点特异性重组酶识别序列引入植物细胞中; 允许重组酶在细胞的至少生长期间暂时在细胞中起作用; 并培养和培养细胞; 并且选择其中预定染色体消失的细胞。

3. 发明申请

US20070277264A1 Novel Vector 审中-公开
标题翻译：小说矢量
公开(公告)号：US20070277264A1
公开(公告)日：2007-11-29
申请号：US10559072
申请日：2004-06-03
申请人： Kazuya Nanto , Keiko Watanabe , Hiroyasu Ebinuma
发明人： Kazuya Nanto , Keiko Watanabe , Hiroyasu Ebinuma
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8213 , C12N15/8209 , C12N15/821
摘要： In order to provide a vector and a method for introducing one copy of a desired gene to a predetermined position of a host DNA at a high frequency without any bad influences upon the host cell and a vector and a method for deleting or inverting a host DNA at a predetermined position without a crossing step and without any bad influences upon the host cell. A vector which has an introduction cassette inserted between two site-specific recombinase recognition sequences and a site-specific recombinase gene which recognizes these site-specific recombinase recognition sequences, wherein the recombinase gene is positioned outside the introduction cassette, is used, and this is introduced into a host cell having a DNA in which one or two site-specific recombinase recognition sequences are present. A recombinant of interest can be efficiently obtained by positioning a lethal induction gene or a morphological abnormality induction gene outside the introduction cassette, together with the site-specific recombinase gene.
摘要翻译：为了提供一种将所需基因的一个拷贝以高频率引入宿主DNA的预定位置的载体和方法，对宿主细胞和载体没有任何不良影响，以及用于删除或翻译宿主DNA的方法在没有交叉步骤的预定位置处，并且对宿主细胞没有任何不良影响。使用具有插入两个位点特异性重组酶识别序列之间的引物盒和识别这些位点特异性重组酶识别序列的位点特异性重组酶基因的载体，其中重组酶基因位于引入盒外部，这是引入具有其中存在一个或两个位点特异性重组酶识别序列的DNA的宿主细胞。通过将导入盒外的致死诱导基因或形态异常诱导基因与位点特异性重组酶基因一起定位，可以有效地获得感兴趣的重组体。

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