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1. 发明授权

US5824474A Method for detecting nucleic acid 失效
标题翻译：检测核酸的方法
公开(公告)号：US5824474A
公开(公告)日：1998-10-20
申请号：US592293
申请日：1996-02-08
申请人： Akio Matsuhisa , Kiyotaka Shiba , Yoshikazu Mikawa , Yuichiro Kishi
发明人： Akio Matsuhisa , Kiyotaka Shiba , Yoshikazu Mikawa , Yuichiro Kishi
IPC分类号： C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6816 , C12Q1/6813
摘要： A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine to which a label capable of generating a detectable signal or a precursor thereof is bound, to form a complex between said nucleic acid and said polyamine, said precursor converting into said label, if used, removing the polyamine which has not formed any complex before or after the conversion of said precursor and then detecting said label.
摘要翻译： PCT No.PCT / JP93 / 01124 Sec。 371日期1996年2月8日 102（e）日期1996年2月8日PCT提交1993年8月10日PCT公布。出版物WO95 / 04832 日期1996年2月16日一种检测核酸的方法，其包括将怀疑携带或含有核酸的固体载体与可产生可检测信号的标记或其前体的多胺接触形成所述核酸和所述多胺之间的复合物，所述前体转化成所述标记（如果使用的话），除去在所述前体转化之前或之后未形成任何复合物的多胺，然后检测所述标记。

2. 发明授权

US08187805B2 Method of detecting nucleic acid and utilization thereof 有权
标题翻译：检测核酸的方法及其利用
公开(公告)号：US08187805B2
公开(公告)日：2012-05-29
申请号：US10588597
申请日：2005-02-08
申请人： Akio Matsuhisa , Seiji Yamamoto , Souji Eda , Shinji Yamasaki
发明人： Akio Matsuhisa , Seiji Yamamoto , Souji Eda , Shinji Yamasaki
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6834 , C12Q1/6841 , C12Q2531/113
摘要： It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.
摘要翻译：旨在提供一种检测核酸的方法，由此与现有方法相比，能够以更高的检测灵敏度准确且快速地检测靶核酸; 以及使用该方法的基因检测试剂盒。将含有细胞的样品固定到载体上，并且核酸在载体上扩增。然后，检测如此扩增的核酸。由于在该方法中未从样品中检测到核酸，所以可以防止在提取核酸的步骤中由于核酸损失引起的检测灵敏度的降低。此外，由于检测到扩增的核酸，所以即使在样品中只含有微量的核酸，也可以进行检测。

3. 发明授权

US09410120B2 Composition for embryo culture 有权
标题翻译：胚胎培养的组成
公开(公告)号：US09410120B2
公开(公告)日：2016-08-09
申请号：US14236534
申请日：2012-07-18
申请人： Tatsuma Yao , Yuta Asayama , Akio Matsuhisa
发明人： Tatsuma Yao , Yuta Asayama , Akio Matsuhisa
IPC分类号： C12N5/00 , C12N1/00 , C12N5/073
CPC分类号： C12N5/0604 , C12N2500/32
摘要： Provided is a composition for embryo culture, which contains a constitution suitable for embryo culture. Provided is a composition for embryo culture, which contains (a) a constitution shown in Table A below. TABLE A ComponentsmM L-Alanine0.297 ± 0.089 L-Asparagine0.015 ± 0.005 L-Aspartic acid0.120 ± 0.036 L-Glutamic acid0.550 ± 0.165 Glycine0.979 ± 0.294 L(−)-Proline0.105 ± 0.032 L-Serine0.176 ± 0.053 L(+)-Arginine0.108 ± 0.032 L(−)-Cystine0.048 ± 0.014 L-Histidine0.053 ± 0.016 L(+)-Isoleucine0.036 ± 0.011 L-Leucine0.081 ± 0.024 L(+)-Lysine0.176 ± 0.053 L-Methionine0.022 ± 0.007 L(−)-Phenylalanine0.045 ± 0.013 L(−)-Threonine0.109 ± 0.033 L-Tryptophan0.018 ± 0.005 L-Tyrosine0.048 ± 0.014 L-Valine0.108 ± 0.032 L-Glutamine or0.398 ± 0.119 glutamine derivative Taurine1.412 ± 0.424
摘要翻译：本发明提供一种胚胎培养用组合物，其含有适合于胚胎培养的构成。本发明提供一种胚胎培养用组合物，其含有（a）下述表A所示的结构。表A组分mM L-丙氨酸0.297±0.089 L-天冬酰胺0.015±0.005 L-天冬氨酸0.120±0.036 L-谷氨酸0.550±0.165甘氨酸0.979±0.294 L（ - ） - 脯氨酸0.105±0.032 L-丝氨酸0.176±0.053L （+） - 精氨酸0.108±0.032 L（ - ） - 胱氨酸0.048±0.014 L-组氨酸0.053±0.016 L（+） - 异亮氨酸0.036±0.011 L-亮氨酸0.081±0.024 L（+） - 赖氨酸0.176±0.053 L-甲硫氨酸 0.022±0.007 L（ - ） - 苯丙氨酸0.045±0.013 L（ - ） - 苏氨酸0.109±0.033 L-色氨酸0.018±0.005 L-酪氨酸0.048±0.014 L-缬氨酸0.108±0.032 L-谷氨酰胺或0.398±0.119谷氨酰胺衍生物牛磺酸1.412 ±0.424

4. 发明授权

US06265568B1 Probes for the diagnosis of infections caused by bacteroides fragilis 有权
标题翻译：用于诊断由脆弱杆菌引起的感染的探针
公开(公告)号：US06265568B1
公开(公告)日：2001-07-24
申请号：US09381849
申请日：2000-01-11
申请人： Hiroshi Ueyama , Kanako Abe , Hiroyuki Keshi , Akio Matsuhisa
发明人： Hiroshi Ueyama , Kanako Abe , Hiroyuki Keshi , Akio Matsuhisa
IPC分类号： C07H2104
CPC分类号： C12Q1/689
摘要： The DNA from the bacteria Bactereoides fragilis is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Bacteroides fragilis, then the sequence of the probe is elucidated.
摘要翻译：提取来自细菌细菌的细菌的DNA，然后用限制酶HindIII完全消化，然后克隆到合适的载体中以选择包含脆弱拟杆菌中基本上含有的DNA的探针，然后阐明探针的序列。

5. 发明授权

US5853998A Probe for diagnosing Enterococcus faecalis 失效
标题翻译：探测肠球菌粪便
公开(公告)号：US5853998A
公开(公告)日：1998-12-29
申请号：US920828
申请日：1997-08-29
申请人： Tsuneya Ohno , Akio Matsuhisa , Hirotsugu Uehara , Soji Eda
发明人： Tsuneya Ohno , Akio Matsuhisa , Hirotsugu Uehara , Soji Eda
IPC分类号： C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： DNA probes for diagnosing infectious diseases involving Enterococcus faecalis and methods of using such probes are provided.
摘要翻译：提供了用于诊断涉及粪肠球菌感染性疾病的DNA探针和使用这种探针的方法。

6. 发明授权

US5798211A Probe for diagnosing Pseudomonas aeruginosa 失效
标题翻译：探测铜绿假单胞菌
公开(公告)号：US5798211A
公开(公告)日：1998-08-25
申请号：US921177
申请日：1997-08-29
申请人： Tsuneya Ohno , Akio Matsuhisa , Hirotsugu Uehara , Soji Eda
发明人： Tsuneya Ohno , Akio Matsuhisa , Hirotsugu Uehara , Soji Eda
IPC分类号： C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： DNA probes for diagnosing infectious diseases involving Pseudomonas aeruginosa and methods of using such probes are provided.
摘要翻译：提供了用于诊断涉及铜绿假单胞菌感染性疾病的DNA探针和使用这种探针的方法。

7. 发明申请

US20140206082A1 COMPOSITION FOR EMBRYO CULTURE 有权
标题翻译：胚胎文化组成
公开(公告)号：US20140206082A1
公开(公告)日：2014-07-24
申请号：US14236534
申请日：2012-07-18
申请人： Tatsuma Yao , Yuta Asayama , Akio Matsuhisa
发明人： Tatsuma Yao , Yuta Asayama , Akio Matsuhisa
IPC分类号： C12N5/073
CPC分类号： C12N5/0604 , C12N2500/32
摘要： Provided is a composition for embryo culture, which contains a constitution suitable for embryo culture. Provided is a composition for embryo culture, which contains (a) a constitution shown in Table A below. TABLE A ComponentsmM L-Alanine0.297 ± 0.089 L-Asparagine0.015 ± 0.005 L-Aspartic acid0.120 ± 0.036 L-Glutamic acid0.550 ± 0.165 Glycine0.979 ± 0.294 L(−)-Proline0.105 ± 0.032 L-Serine0.176 ± 0.053 L(+)-Arginine0.108 ± 0.032 L(−)-Cystine0.048 ± 0.014 L-Histidine0.053 ± 0.016 L(+)-Isoleucine0.036 ± 0.011 L-Leucine0.081 ± 0.024 L(+)-Lysine0.176 ± 0.053 L-Methionine0.022 ± 0.007 L(−)-Phenylalanine0.045 ± 0.013 L(−)-Threonine0.109 ± 0.033 L-Tryptophan0.018 ± 0.005 L-Tyrosine0.048 ± 0.014 L-Valine0.108 ± 0.032 L-Glutamine or0.398 ± 0.119 glutamine derivative Taurine1.412 ± 0.424
摘要翻译：本发明提供一种胚胎培养用组合物，其含有适合于胚胎培养的构成。本发明提供一种胚胎培养用组合物，其含有（a）下述表A所示的结构。表A组分mM L-丙氨酸0.297±0.089 L-天冬酰胺0.015±0.005 L-天冬氨酸0.120±0.036 L-谷氨酸0.550±0.165甘氨酸0.979±0.294 L（ - ） - 脯氨酸0.105±0.032 L-丝氨酸0.176±0.053L （+） - 精氨酸0.108±0.032 L（ - ） - 胱氨酸0.048±0.014 L-组氨酸0.053±0.016 L（+） - 异亮氨酸0.036±0.011 L-亮氨酸0.081±0.024 L（+） - 赖氨酸0.176±0.053 L-甲硫氨酸 0.022±0.007 L（ - ） - 苯丙氨酸0.045±0.013 L（ - ） - 苏氨酸0.109±0.033 L-色氨酸0.018±0.005 L-酪氨酸0.048±0.014 L-缬氨酸0.108±0.032 L-谷氨酰胺或0.398±0.119谷氨酰胺衍生物牛磺酸1.412 ±0.424

8. 发明授权

US07651837B2 Method for detecting and identifying microorganism causative of infection 有权
标题翻译：检测和鉴定感染微生物的方法
公开(公告)号：US07651837B2
公开(公告)日：2010-01-26
申请号：US10479185
申请日：2002-05-27
申请人： Tsuneya Ohno , Akio Matsuhisa
发明人： Tsuneya Ohno , Akio Matsuhisa
IPC分类号： C12Q1/68 , C12Q1/37 , G01N1/30
CPC分类号： C12Q1/689 , C12Q1/04
摘要： Causative microorganisms of infectious diseases are detected and/or identified rapidly and with high sensitivity by taking phagocytes from clinical specimens containing active phagocytes, immobilizing the phagocytes so taken, treating the phagocytes to improve cell membrane permeabilities thereof, further treating the phagocytes to bare DNA in the causative microorganisms which might exist in the phagocytes, and detecting the causative microorganisms with DNA probes which can hybridize with such DNA under stringent conditions.
摘要翻译：通过从包含活细胞吞噬细胞的临床标本中取出吞噬细胞，固定吞噬细胞，检测吞噬细胞，改善细胞膜渗透性，进一步将吞噬细胞处理成裸露的DNA，从而快速检测和/或识别感染性疾病的致病微生物，可能存在于吞噬细胞中的致病微生物，以及在严格条件下与DNA探针杂交的DNA探针检测致病微生物。

9. 发明申请

US20070111212A1 Method of detecting nucleic acid and utilization thereof 有权
标题翻译：检测核酸的方法及其利用
公开(公告)号：US20070111212A1
公开(公告)日：2007-05-17
申请号：US10588597
申请日：2005-02-08
申请人： Akio Matsuhisa , Seiji Yamamoto , Souji Eda , Shinji Yamasaki
发明人： Akio Matsuhisa , Seiji Yamamoto , Souji Eda , Shinji Yamasaki
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6834 , C12Q1/6841 , C12Q2531/113
摘要： It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.
摘要翻译：旨在提供一种检测核酸的方法，由此与现有方法相比，能够以更高的检测灵敏度准确且快速地检测靶核酸; 以及使用该方法的基因检测试剂盒。将含有细胞的样品固定到载体上，并且核酸在载体上扩增。然后，检测如此扩增的核酸。由于在该方法中未从样品中检测到核酸，所以可以防止在提取核酸的步骤中由于核酸损失引起的检测灵敏度的降低。此外，由于检测到扩增的核酸，所以即使在样品中只含有微量的核酸，也可以进行检测。

10. 发明申请

US20060234219A1 Method for detecting and identifying microorganism causative of infection 有权
标题翻译：检测和鉴定感染微生物的方法
公开(公告)号：US20060234219A1
公开(公告)日：2006-10-19
申请号：US10479185
申请日：2002-05-27
申请人： Tsuneya Ohno , Akio Matsuhisa
发明人： Tsuneya Ohno , Akio Matsuhisa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/04
摘要： Causative microorganisms of infectious diseases are detected and/or identified rapidly and high-sensitively by taking phagocytes from the clinical specimens containing active phagocytes, immobilizing the phagocytes so taken, treating the phagocytes to improve cell membrane permeabilities thereof, further treating the phagocytes to bare DNA in the causative microorganisms which might be existed in the phagocytes, and detecting the causative microorganisms with DNA probes which can hybridize with such DNA under stringent conditions.
摘要翻译：通过从含有活细胞吞噬细胞的临床标本中取出吞噬细胞，固定吞噬细胞，检测吞噬细胞以改善细胞膜渗透性，进一步将吞噬细胞处理为裸DNA，来检测和/或识别快速且高敏感性的感染性疾病的致病微生物在可能存在于吞噬细胞中的致病微生物中，以及在严格条件下用DNA探针检测致病微生物。

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