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1. 发明授权

US5476791A Process for producing optically active carboxylic acid amide 失效
标题翻译：光学活性羧酸酰胺的制备方法
公开(公告)号：US5476791A
公开(公告)日：1995-12-19
申请号：US243521
申请日：1994-05-16
申请人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
发明人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
IPC分类号： C07D207/16 , C07D277/06 , C12P7/40 , C12P41/00 , C12P9/00
CPC分类号： C12P7/40 , C07D207/16 , C07D277/06 , C12P41/005 , Y10S435/822
摘要： N-(3-acetylthio-2-D-methylpropionyl)-L-proline useful as a precursor of captopril, one of antihypertensive agents, is prepared by allowing thioacetic acid to react with methyl methacrylate to prepare racetalc methyl 3-acetylthio-2-DL-methylpropionate, contacting the propionate with cells of, for example, Pseudomonas in order to effect biological and asymmetric hydrolysis, extracting 3-acetylthio-D-2-methylpropionic acid and allowing the acid to react with L-proline.
摘要翻译：通过使硫代乙酸与甲基丙烯酸甲酯反应制备N-乙酰基-3-乙酰硫基-2-脯氨酸，其是抗高血压药之一，可用作卡托普利前体的N-（3-乙酰硫基-2-D-甲基丙酰基）-L-脯氨酸， DL-甲基丙酸酯，使丙酸酯与例如假单胞菌的细胞接触以进行生物和不对称水解，提取3-乙酰硫基-D-2-甲基丙酸，并使酸与L-脯氨酸反应。

2. 发明授权

US4629701A Process for preparing optically active carboxylic acids and antipode esters thereof 失效
标题翻译：光学活性羧酸及其对映体酯的制备方法
公开(公告)号：US4629701A
公开(公告)日：1986-12-16
申请号：US627093
申请日：1984-07-02
申请人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
发明人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
IPC分类号： C12P11/00 , C12P41/00
CPC分类号： C12P41/00 , C12P11/00
摘要： Organic carboxylic acid ester represented by the formula (II): ##STR1## wherein R.sub.1 is an alkyl, an aralkyl or an aryl group; R.sub.2 is an alkyl group; R.sub.3 is an alkyl group; n is 1 or 2, are synthesized in racemic form, and then the acid ester (II) is treated with a source containing enzyme capable of rearranging with or without asymmetrically hydrolyzing ester bond until either a d-form or 1-form optically active carboxylic acid ester represented by the formula (II) with or without the antipode acid thereof is produced.
摘要翻译：由式（II）表示的有机羧酸酯：其中R 1是烷基，芳烷基或芳基; R2是烷基; R3是烷基; n为1或2，以外消旋形式合成，然后用含有酶的酶处理酸酯（II），其能够或不具有不对称水解的酯键重排，直到d型或1-型光学活性羧基制备了具有或不具有对映体酸的由式（II）表示的酸酯。

3. 发明授权

US4885244A Method of producing hyaluronic acid 失效
标题翻译：生产透明质酸的方法
公开(公告)号：US4885244A
公开(公告)日：1989-12-05
申请号：US104219
申请日：1987-10-05
申请人： Takao Miyamori , Ryozo Numazawa , Akihiro Sakimae , Hisao Onishi
发明人： Takao Miyamori , Ryozo Numazawa , Akihiro Sakimae , Hisao Onishi
IPC分类号： C12P19/04 , C08B37/08 , C12N1/20 , C12P19/26 , C12R1/46
CPC分类号： C08B37/0072 , C12P19/26 , Y10T436/188
摘要： High molecular weight hyaluronic acid can be efficiently produced by incorporating one or more compounds having at least one aromatic ring to which one or more hydroxyl groups are attached into a medium for producing hyaluronic acid by a bacterium capable of producing hyaluronic acid. By using this method, a sufficient amount of hyaluronic acid can be easily obtained without decomposing the high molecular structure of hyaluronic acid so that the process for isolating and purifying hyaluronic acid from the medium can be simplified.
摘要翻译：高分子量透明质酸可以通过将一种或多种具有至少一个芳环的化合物（其中一个或多个羟基被连接到用于产生透明质酸的细菌的培养基中）而被能够产生透明质酸的细菌产生。通过使用该方法，可以容易地获得足够量的透明质酸，而不会分解透明质酸的高分子结构，从而可以简化从培养基中分离和纯化透明质酸的方法。

4. 发明授权

US5079153A Process for the preparation of organic esters of ascorbic acid or erythorbic acid using ester hydralases in organic solvents containing 100-10,000 ppm of water 失效
标题翻译：在含有100-10,000 PPM水的有机溶剂中使用酯类水合物制备抗坏血酸或硫酸的有机酸的方法
公开(公告)号：US5079153A
公开(公告)日：1992-01-07
申请号：US531701
申请日：1990-06-01
申请人： Kanehiko Enomoto , Takao Miyamori , Akihiro Sakimae , Ryozo Numazawa
发明人： Kanehiko Enomoto , Takao Miyamori , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I) :R.sub.1 COOR.sub.2 (I)wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.

5. 发明授权

US5071753A Process for the enzymatic preparation of organic esters of ascorbic acid or erythorbic acid 失效
标题翻译：抗坏血酸或硫酸的有机酸的制备方法
公开(公告)号：US5071753A
公开(公告)日：1991-12-10
申请号：US607676
申请日：1990-11-01
申请人： Kanehiko Enomoto , Takao Miyamori , Akihiro Sakimae , Ryozo Numazawa
发明人： Kanehiko Enomoto , Takao Miyamori , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I):wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.

6. 发明授权

US5473081A Process for the preparation of l-proline derivatives 失效
标题翻译：制备脯氨酸衍生物的方法
公开(公告)号：US5473081A
公开(公告)日：1995-12-05
申请号：US181168
申请日：1994-01-13
申请人： Yoshimasa Kobayashi , Kaori Tosa , Akihiro Sakimae , Ryozo Numazawa
发明人： Yoshimasa Kobayashi , Kaori Tosa , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C07D207/16 , C07D207/12
CPC分类号： C07D207/16
摘要： An L-proline derivative represented by: ##STR1## wherein R.sub.2 represents a lower alkyl group is prepared by reacting L-proline with a D-.alpha.-alkyl-.beta.-acylthiopropionic acid halide, ##STR2## wherein R.sub.1 represents an acyl group, X represents a halogen atom and R.sub.2 has the same meaning as defined above. The halide is added to an aqueous medium containing L-proline and a condensation agent for deacidification. The halide and L-proline are reacted while the reaction temperature and pH are maintained at 12.degree. C. or lower and within a range of 10.5-11.5, whereby a compound represented by: ##STR3## wherein R.sub.1 and R.sub.2 have the same meanings as defined above is obtained. The resulting compound is then subjected to deacylation under strong alkaline conditions without being isolated from the reaction mixture, thereby yielding the target L-proline derivative.
摘要翻译：由下式表示的L-脯氨酸衍生物：其中R2表示低级烷基，其通过L-脯氨酸与D-α-烷基-β-酰基硫代丙酰卤反应来制备，其中R1表示酰基，X 表示卤素原子，R2表示与上述相同的含义。将卤化物加入到含有L-脯氨酸和用于脱酸的缩合剂的水性介质中。使卤化物和L-脯氨酸反应，同时将反应温度和pH保持在12℃或更低并且在10.5-11.5的范围内，由此得到由下式表示的化合物：其中R1和R2具有相同的含义获得上述定义。然后将所得化合物在强碱性条件下进行脱酰基，而不从反应混合物中分离，从而得到目标L-脯氨酸衍生物。

7. 发明授权

US5482847A Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters using said trasnformants 失效
标题翻译：酯酶基因，酯酶，重组质粒和含有重组质粒的转化体和使用所述trasnformants制备光学活性羧酸及其对映体酯的方法
公开(公告)号：US5482847A
公开(公告)日：1996-01-09
申请号：US183213
申请日：1994-01-14
申请人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
发明人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C12N9/18 , C12N15/55 , C12P7/40 , C12P11/00 , C12P41/00 , C12N9/16 , C07K14/21
CPC分类号： C12P11/00 , C12N9/18 , C12P41/005 , C12P7/40
摘要： The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.
摘要翻译：本发明涉及包含编码酯酶氨基酸序列的核苷酸序列的DNA片段，所述酯酶不对称水解的由式（I）表示的羧酸酯; （其中R1是烷基，芳烷基或芳基，R2和R3是烷基，n是1或2）由DNA片段编码的酯酶，含有该DNA片段的重组质粒，用重组质粒转化的微生物和制备光学活性羧酸及其对映体酯的方法。

8. 发明授权

US5308765A Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically acitve carboxylic acids and their enantiomeric esters using said transformants 失效
标题翻译：酯酶基因，酯酶，重组质粒和含有重组质粒的转化体和使用所述转化体产生光学酸性羧酸及其对映体酯的方法
公开(公告)号：US5308765A
公开(公告)日：1994-05-03
申请号：US882329
申请日：1992-05-13
申请人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
发明人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C12N9/18 , C12N15/55 , C12P7/40 , C12P11/00 , C12P41/00 , C12A15/00
CPC分类号： C12P11/00 , C12N9/18 , C12P41/005 , C12P7/40
摘要： The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.
摘要翻译：本发明涉及包含编码酯酶氨基酸序列的核苷酸序列的DNA片段，所述酯酶不对称水解的由式（I）表示的羧酸酯; （其中R1是烷基，芳烷基或芳基，R2和R3是烷基，n是1或2）由DNA片段编码的酯酶，含有该DNA片段的重组质粒，用重组质粒转化的微生物和制备光学活性羧酸及其对映体酯的方法。

9. 发明授权

US5149855A Process for racemizing optically active carboxylic acid esters 失效
标题翻译：用于驯化光活性羧酸酯的方法
公开(公告)号：US5149855A
公开(公告)日：1992-09-22
申请号：US627779
申请日：1990-12-14
申请人： Akihiro Sakimae , Eiji Ozaki , Kanehiko Enomoto , Ryozo Numazawa , Yoshimasa Kobayashi
发明人： Akihiro Sakimae , Eiji Ozaki , Kanehiko Enomoto , Ryozo Numazawa , Yoshimasa Kobayashi
IPC分类号： C07C327/32
CPC分类号： C07C327/32
摘要： Process for racemizing an optically active carboxylic acid ester of the formula (1): ##STR1## wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 independently are alkyl, and n is 1 or 2, which comprises contacting the compound of the formula (1) with an amine compound.

10. 发明授权

US5149649A Multi-layered porous hollow fiber membrane for use in cell culture 失效
标题翻译：用于细胞培养的多层多孔中空纤维膜
公开(公告)号：US5149649A
公开(公告)日：1992-09-22
申请号：US561323
申请日：1990-08-01
申请人： Takao Miyamori , Makoto Uchida , Kanehiko Enomoto , Akihiro Sakimae , Ryozo Numazawa
发明人： Takao Miyamori , Makoto Uchida , Kanehiko Enomoto , Akihiro Sakimae , Ryozo Numazawa
IPC分类号： C12M3/00 , C12M1/04 , C12M1/08 , C12M3/06 , C12N1/20
CPC分类号： C12M29/04 , C12M23/24 , C12M41/02
摘要： A method for culturing cells by supplying oxygen to a culture medium through a multilayer composite membrane consisting of a porous layer(s) and a nonporous layer(s) having a thickness of less than 10 micrometers laminated one after the other. By using this multilayer composite membrane for supplying oxygen to the culture medium, oxygen can be supplied to the culture medium and/or the culture broth without causing foaming in the culture medium, cell growth inhibition or reduction in productivity of cellular products.

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